Pilus F
Mostrando 1-12 de 155 artigos, teses e dissertações.
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1. Detecção molecular de Escherichia coli enteropatogênica em psitacídeos assintomáticos em cativeiro
Os psitacídeos são um dos grupos de aves mais ameaçadas no mundo e diversas espécies brasileiras são classificadas desde vulneráveis à criticamente ameaçadas de extinção. Torna-se, portanto, necessário identificar os agentes que causam infecções em animais selvagens em cativeiro e determinar os riscos relacionados de modo a intervir sobre os fat
Pesq. Vet. Bras.. Publicado em: 2012-09
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2. Estudo estrutural e funcional das proteínas PilZ e YaeQ do fitopatógeno Xanthomonas axonopodis pv citri / Structural and functional studies of PilZ and YaeQ from Xanthomonas axonopodis pv citri proteins
The aim of the project was to perform structural and functional studies of different Xanthomonas axonopodis pv citri (Xac) proteins including the hypothetical proteins YaeQ and SufE; RpfC, RpfF and RpfG involved in the quorum sensing and PilZ, FimX and PilB that play roles in type IV pilus (T4P) biogenesis. Several experimental techniques were employed inclu
Publicado em: 2010
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3. Patogênese de Escherichia coli enteroagregativa : o papel de supostos pili F na intensificação de biofilmes mistos e na adesão a células HeLa
Escherichia coli enteroagregativa (EAEC) é uma categoria diarreiogênica composta por isolados que expressam o característico padrão de adesão agregativa (AA) em associação com células epiteliais. Nas cepas protótipo de EAEC, o fenótipo AA está associado à presença dos plasmídios pAA que codificam para fímbrias de adesão agregativa (AAF). Não
Publicado em: 2009
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4. Location of the antigenic determinants of conjugative F-like pili.
The amino terminus of the pilin protein constitutes the major epitope of F-like conjugative pili studied to date (F, ColB2, R1-19, R100-1, and pED208). Anti-pED208 pilus antibodies were passed through a CNBr-Sepharose affinity column linked to bovine serum albumin which was conjugated to a synthetic peptide, AcP(1-12), containing the major epitope at the ami
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5. F41 pili as protective antigens of enterotoxigenic Escherichia coli that produce F41, K99, or both pilus antigens.
Pigs suckling dams that have been vaccinated with pilus antigen are protected against challenge with enterotoxigenic Escherichia coli (ETEC) strains that express the same pilus antigen. However, some ETEC strains express more than one pilus antigen. Pregnant swine were vaccinated either with E. coli HB101 that harbored a recombinant plasmid coding for F41 ex
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6. Identification and characterization of genes determining receptor binding and pilus length of Escherichia coli type 1 pili.
We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pilus length, in that mutants having lesion
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7. Characterization and sequence analysis of pilin from F-like plasmids.
Conjugative pili are expressed by derepressed plasmids and initiate cell-to-cell contact during bacterial conjugation. They are also the site of attachment for pilus-specific phages (f1, f2, and QB). In this study, the number of pili per cell and their ability to retract in the presence of cyanide was estimated for 13 derepressed plasmids. Selected pilus typ
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8. Structure and antigenic properties of the tip-located P pilus proteins of uropathogenic Escherichia coli.
Pyelonephritogenic Escherichia coli frequently expresses pili which bind to Gal alpha (1-4)Gal receptors present on the uroepithelium. Binding of these pili is mediated by a pilus-associated adhesin, PapG, and not by the major subunit which constitutes the bulk of the pilus structure. The adhesin and two pilinlike proteins, PapE and PapF, are present in only
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9. Naturally Occurring R Factor, Derepressed for Pilus Synthesis, Belonging to the Same Compatibility Group as the Sex Factor F of Escherichia coli K-12
A naturally occurring R factor with constitutive pilus synthesis is described which resembles the sex factor F in compatibility and in restricting coliphage T7. Unlike F, it is not cured during growth with acridine orange. Results suggest that the R factor produces repressor of pilus synthesis, to which the operator is insensitive (i+oc). In this respect it
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10. Differentiation of arcA, arcB, and cpxA mutant phenotypes of Escherichia coli by sex pilus formation and enzyme regulation.
In Escherichia coli, mutations in arcA (dye) or arcB anaerobically derepress the synthesis of a multitude of enzymes of aerobic function, and mutations in arcA or cpxA impair F-pilus formation. It is thought that arcA encodes a promoter-recognizing protein, whereas arcB and cpxA encode sensor proteins which interact with the arcA product. In this study we fo
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11. Characterization of the F-plasmid conjugative transfer gene traU.
We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped between traW and trbC. It encodes a 330-amino-a
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12. Characterization, localization, and sequence of F transfer region products: the pilus assembly gene product TraW and a new product, TrbI.
The traW gene of the Escherichia coli K-12 sex factor, F, encodes one of the numerous proteins required for conjugative transfer of this plasmid. We have found that the nucleotide sequence of traW encodes a 210-amino-acid, 23,610-Da polypeptide with a characteristic amino-terminal signal peptide sequence; in DNA from the F lac traW546 amber mutant, the traW