Pluripotency Markers
Mostrando 1-11 de 11 artigos, teses e dissertações.
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1. Embryonic stem-like cells derived from in vitro produced bovine blastocysts
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissecte
Brazilian Archives of Biology and Technology. Publicado em: 2011-06
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2. Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up
Brazilian Journal of Medical and Biological Research. Publicado em: 2009-06
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3. Identificação de marcadores de pluripotência em células-tronco embrionárias e embriões suínos / Identification of pluripotency markers in swine embryonic stem cells and embryos
Células-tronco embrionárias (CTE) são importantes para estudos de desenvolvimento embrionário, diferenciação e manipulação genética. Além disso, essas células podem ser utilizadas na terapia celular e organogênese in vitro. Na pesquisa sobre terapia celular a partir de CTE oriundas de embriões humanos, considerações éticas, morais e religiosa
Publicado em: 2009
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4. Uso de matriz extracelular (Matrigel®) para estabelecimento de cultivo de células-tronco embrionárias de suínos e caracterização da expressão de moléculas associadas à pluripotência / Use of extracellular matrix (Matrigel®) for establishment of porcine embryonic stem cells and expression characterization of plurpotency related molecules
Establishment of embryonic stem cell (ESC) culture in pigs has not been achieved. Verification of pluripotency markers and differentiation in the three embryonic layers are necessary for validation of a pluripotent cell line. The objective of this study was to establish and characterize porcine ESC culture using Matrigel and compare the expression of pluripo
Publicado em: 2008
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5. Gene Expression Profiling of Embryo-Derived Stem Cells Reveals Candidate Genes Associated With Pluripotency and Lineage Specificity
Large-scale gene expression profiling was performed on embryo-derived stem cell lines to identify molecular signatures of pluripotency and lineage specificity. Analysis of pluripotent embryonic stem (ES) cells, extraembryonic-restricted trophoblast stem (TS) cells, and terminally-differentiated mouse embryo fibroblast (MEF) cells identified expression profil
Cold Spring Harbor Laboratory Press.
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6. Maintenance of pluripotency in mouse embryonic stem cells persistently infected with murine coronavirus.
A persistently coronavirus-infected embryonic stem (ES) cell line A3/MHV was established by infecting an ES cell line, A3-1, with mouse hepatitis virus type-2. Although almost all A3/MHV cells were found infected, both A3/MHV and A3-1 cells expressed comparable levels of cell surface differentiation markers. In addition, A3/MHV cells retained the ability to
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7. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Quantitative Comparison of the Membrane Proteomes of Self-renewing and Differentiating Human Embryonic Stem Cells*S⃞
Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful quantitative proteomics platform for comprehensive characterization of complex biological systems. However, the potential of SILAC-based approaches has not been fully utilized in human embryonic stem cell (hESC) research mainly because of the complex nature of hESC culture condition
American Society for Biochemistry and Molecular Biology.
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8. Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig*
Induced pluripotent stem cell (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat, and monkey. These four species have also established embryonic stem cell (ESC) lines that serve as the gold standard for pluripotency comparisons. Atte
American Society for Biochemistry and Molecular Biology.
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9. Cardiomyocytes induce endothelial cells to trans-differentiate into cardiac muscle: Implications for myocardium regeneration
The concept of tissue-restricted differentiation of postnatal stem cells has been challenged by recent evidence showing pluripotency for hematopoietic, mesenchymal, and neural stem cells. Furthermore, rare but well documented examples exist of already differentiated cells in developing mammals that change fate and trans-differentiate into another cell type.
The National Academy of Sciences.
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10. Sox2 is dispensable for the reprogramming of melanocytes and melanoma cells into induced pluripotent stem cells
Induced pluripotent stem cells (iPSCs) have been derived at low frequencies from different cell types through ectopic expression of the transcription factors Oct4 and Sox2, combined with either Klf4 and c-Myc or Lin28 and Nanog. In order to generate iPSCs more effectively, it will be crucial to identify somatic cells that are easily accessible and possib
Company of Biologists.
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11. Oct4 distribution and level in mouse clones: consequences for pluripotency
Somatic cell clones often fail at a developmental stage coincident with commencement of differentiation. The transcription factor Oct4 is expressed during cleavage stages and is essential for the differentiation of the blastocyst. Oct4 expression becomes restricted to the inner cell mass and epiblast. After gastrulation Oct4 is active only in germ cells and
Cold Spring Harbor Laboratory Press.