Pnp Enzyme
Mostrando 1-12 de 47 artigos, teses e dissertações.
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1. Changes in hepatic metabolic enzyme activities and biliary excretion of 4-nitrophenol in streptozotocin induced diabetic rats
Abstract Activity of hepatic metabolic enzymes of glucuronidation and sulfation of 4-nitrophenol (PNP) and biliary excretion of its glucuronide (PNP-G) and sulfate (PNP-S) conjugates have been investigated in control and streptozotocin (STZ)-induced diabetic rats. 500 μM PNP solution was luminally perfused in a cannulated jejunal loop for 90 minutes. It was
Braz. J. Pharm. Sci.. Publicado em: 07/06/2018
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2. Desenvolvimento e aplicação de biorreatores capilares para a triagem de ligantes de Purina Nucleosídeo Fosforilases / Development and application of capillary bioreactors for screening of Purine Nucleoside Phosphorylases ligands
Purine Nucleoside Phosphorylases (PNPs) are key enzymes of the purine salvage pathway, and therefore are considered attractive targets for new drugs search. In this context, the development of effective and selective bioassays for PNP ligands screening is an important task. This work describes the covalent immobilization of human and Schistossoma mansoni PNP
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2012
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3. Enzyme kinetics, structural analysis and molecular modeling studies on a series of Schistosoma mansoni PNP inhibitors
A enzima purina nucleosídeo fosforilase do parasita Schistosoma mansoni (SmPNP) é um alvo molecular atrativo para o desenvolvimento de candidatos a novos fármacos para o tratamento da esquistossomose, doença tropical negligenciada que afeta mais de 200 milhões de pessoas em todo mundo. No presente trabalho, estudos de cinética enzimática foram conduzi
Journal of the Brazilian Chemical Society. Publicado em: 2011-03
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4. Synthesis and enzymatic evaluation of the guanosine analogue 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). Insights into the phosphorolysis reaction mechanism based on the blueprint transition state: sn1 or sn2
A modificação experimental para a síntese do MESG (2-amino-6-mercapto-7-metilpurina ribonucleosídeo) 1 foi realizada com sucesso e sua caracterização total apresentada. ESI(+)-MSMS em alta resolução foram realizados indicando que a clivagem nucleosídica como principal e um possível mecanismo SN1. Cálculos ab initio baseados em estados de transiç�
Publicado em: 2011
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5. Estudo visando a síntese enantiosseletiva da dictiolomida e da 6-metoxidictiolomida : síntese e avaliação biológica de derivados quinolinônicos / TOWARDS OF ENANTIOSELECTIVE SYNTHESIS OF DICTYOLOMIDE A AND 6-METHOXIDICTYOLOMIDE: SYNTHESIS AND BIOLOGICAL EVALUATION OF QUINOLINONICS DERIVATIVES
Dictyolomide A (13) and 6-methoxydictyolomide (15) were isolated from plants of the Dictyoloma genus, therefore their structures were not yet completely elucidated due to the C-3 stereocenter. These compounds showed an interesting biological activity against different forms of Leishmania sp., protozoa responsible for leishmaniose. In this work is described t
Publicado em: 2007
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6. Caracterização bioquímica das ß-glucosidases do Scytalidium thermophilum / Biochemical characterization of ß-glucosidases from Scytalidium thermophilum
Cellulose is the most abundant carbon source found in woods and waste residues. In nature the complete hidrolysis of cellulose occurs by the sinergistic action of several enzymes such endo-1,4-ß-D-glucanase, exo-1,4-ß-glucanase e ß-glucosidase or cellobiase. The present work describe some physiological and biochemical properties of ß-glucosidase system f
Publicado em: 2005
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7. Purine nucleoside fosforilase from Schistosoma Mansoni: crystal structure, knetics, studies and ligands search / Enzima purina nucleosideo fosforilase de Schistosoma Mansoni: estruturas cristalográficas, estudos cinéticos e descoberta de novos ligantes
O parasita Schistosoma mansoni não possui a via de síntese de bases púricas e depende integralmente da via de salvação de purinas para o seu requerimento de purinas. Uma das enzimas participantes desta via é a Purina Nucleosídeo Fosforilase (PNP) (E.C. 2.4.2.1). A PNP catalisa a fosforólise reversível de nucleosídeos de purina para gerar a base cor
Publicado em: 2003
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8. Estudos cineticos, fisico-quimicos e conformacional da fosfatase acida de sementes de mamona (Ricinus communis)
This work contains results about kinetic, physico-chemical and conformationalstudies of castor bean seed acid phosphatase. About kinetic approaches we studied the effect of inhibitors and determined the amino acids present in the active site. Inorganic phosphate, molybidate and o vanadate were competitive inhibitors, with Ki values of 0,14 mM, 10 x 10-6 mM e
Publicado em: 2001
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9. Produção, purificação e caracterização de a-galactosidase fungica. Hidrolise de oligossacarides do extrato de soja
The production, of α-galactcsidase by a strain of Aspergillus orizas was observed either by sub merged culture method or Koji method. The enzyme was slightly induced by raffinose and stacchyosef while in a previously fermented oligosaccharides extract from soybean with 3.7 per cent of total sugar the yield was greatly increased. The partially purified &
Publicado em: 1983
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10. Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12.
We report the purification and enzymological characterization of Escherichia coli K-12 pyridoxine (pyridoxamine) 5'-phosphate (PNP/PMP) oxidase, which is a key committed enzyme in the biosynthesis of the essential coenzyme pyridoxal 5'-phosphate (PLP). The enzyme encoded by pdxH was overexpressed and purified to electrophoretic homogeneity by four steps of c
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11. Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol.
Modification by covalent attachment of polyethylene glycol (PEG) can reduce the immunogenicity and prolong the circulating life of proteins, but the utility of this approach for any protein is restricted by the number and distribution of PEG attachment sites (e.g., epsilon-amino groups of lysine residues). We have developed a strategy for introducing additio
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12. Design of vectors for efficient expression of human purine nucleoside phosphorylase in skin fibroblasts from enzyme-deficient humans.
Purine nucleoside phosphorylase (PNP; purine-nucleoside orthophosphate ribosyltransferase, EC 2.4.2.1) deficiency is an inherited disorder associated with a severe immune defect that is fatal. Enzyme replacement therapy is an attractive approach to treatment of this disease. To this aim we constructed retroviral vectors containing a human PNP cDNA and a sele