Polyclonal Antibody Preparation
Mostrando 1-12 de 49 artigos, teses e dissertações.
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1. Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Abstract Background The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017
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2. Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Abstract Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 22/09/2016
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3. Performance, carcass characteristics and gain cost of feedlot cattle fed a high level of concentrate and different feed additives
The objective of this study was to evaluate the effects of feeding cattle with isoprotein and isoenergetic diets, with and without the addition of polyclonal antibody preparation (PAP), yeasts (YST) or monensin sodium (MON) on performance, carcass characteristics and gain cost in feedlot. Ninety-five 20-month old bullocks (323.3±21.8 kg) were distributed in
R. Bras. Zootec.. Publicado em: 2013-01
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4. Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg
A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, a
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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5. Evaluation of microbial diversity and in situ degradability in animals treated with polyclonal antibody preparation against lactate-producer and proteolytic bacteria / Avaliação da diversidade microbiana e degradabilidade in situ em animais tratados com preparado de anticorpos policlonais contra bactérias produtoras de lactato e bactérias proteolíticas
A imunidade passiva surge como uma alternativa para a manipulação da fermentação ruminal e anticorpos de origem aviária contra bactérias específicas começam a ser pesquisados. Objetivou-se com este trabalho avaliar um preparado de anticorpos policlonais contra Streptococus bovis, Fusobacterium necrophorum e algumas cepas de bactérias proteolíticas
Publicado em: 2008
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6. Distribution of versican and hyaluronan in the mouse uterus during decidualization
Preparation for embryo implantation requires extensive adaptation of the uterine microenvironment. This process consists of cell proliferation and cell differentiation resulting in the transformation of endometrial fibroblasts into a new type of cell called decidual cell. In the present study, we followed the space-time distribution of versican and hyalurona
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-08
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7. Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents.
Polyclonal and monoclonal antibodies against capsular polysaccharides of Neisseria meningitidis serogroups A, B, and C were produced in order to develop immunological reagents allowing both the detection of soluble antigens during meningococcal meningitis and antigenic serogrouping of N. meningitidis cultures. The performance characteristics of monoclonal an
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8. Enzyme-linked immunosorbent assay for shigella toxin.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of shigella toxin. For the assay, a mouse monoclonal antibody against the B subunit of the toxin and a rabbit polyclonal antibody against the holotoxin were employed. The monoclonal antibody was used to coat wells of a microtiter plate, and the polyclonal antibody preparation was us
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9. Polyclonal and monoclonal antibody therapy for experimental Pseudomonas aeruginosa pneumonia.
A human immunoglobulin G preparation, enriched in antibodies to lipopolysaccharide (LPS) Pseudomonas aeruginosa antigens (PA-IGIV) and murine monoclonal antibodies (MAb) to P. aeruginosa Fisher immunotype-1 (IT-1) LPS antigen and outer membrane protein F (porin), were evaluated for therapeutic efficacy in a guinea pig model of P. aeruginosa pneumonia. The co
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10. Action of antibodies directed against the acetylcholine receptor on channel function at mouse and rat motor end-plates.
1. The acute effects of antibodies (both polyclonal and monoclonal) raised against the acetylcholine receptor were studied at mouse and rat end-plates. Isolated muscles were incubated in solutions containing antibody for 2 1/4 to 3 1/2 h. Intracellular microelectrode techniques were then used to record miniature end-plate potentials (MEPPs) and voltage noise
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11. Immunocytochemical localization of enterobacterial common antigen in Escherichia coli and Yersinia enterocolitica cells.
Enterobacterial common antigen (ECA) was localized on Lowicryl K4M sections and on ultrathin cryosections by using either a mouse monoclonal antibody or an absorbed rabbit polyclonal immune serum with the corresponding gold-labeled secondary antibodies. Comparable results were obtained with both monoclonal antibody and polyclonal immune serum. Controls with
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12. JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
A new monoclonal antibody, JC70, raised against a membrane preparation from a spleen affected by hairy cell leukaemia, recognises a membrane bound glycoprotein identical with that of the CD31 group of monoclonal antibodies. The antibody stains a fixation resistant epitope on endothelial cells in benign and malignant conditions in a wide variety of paraffin w