Polytene Chromosomes
Mostrando 1-12 de 238 artigos, teses e dissertações.
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1. Anopheles darlingi polytene chromosomes: revised maps including newly described inversions and evidence for population structure in Manaus
Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, tw
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-05
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2. Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand
To assess differentiation and relationships between Anopheles lesteri and Anopheles paraliae we established three and five iso-female lines of An. lesteri from Korea and An. paraliae from Thailand, respectively. These isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing DNA sequences of ri
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-05
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3. 5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)
5-Bromo-2’-deoxyuridine (BrdUrd) has long been known to interfere with cell differentiation. We found that treatment ofBradysia hygida larvae with BrdUrd during DNA puff anlage formation in the polytene chromosomes of the salivary gland S1 region noticeably affects anlage morphology. However, it does not affect subsequent metamorphosis to the adult stage.
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-12
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4. DNA repetitivo e seu papel na estrutura cromossômica terminal em Rhynchosciara americana (Diptera: Sciaridae) / The role of repetitive DNA in the chromosome termini of Rhynchosciara americana (Diptera: Sciaridae)
The chromosomal localization of ribosomal DNA (rDNA) was studied in polytene and diploid tissues of four sciarid species, Trichosia pubescens, Rhynchosciara americana, R. milleri and Schwenkfeldina sp. While hybridization to mitotic chromosomes showed the existence of a single rDNA locus, ribosomal probes hybridized to more than one polytene chromosome regio
Publicado em: 2008
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5. Polytene chromosomes and phylogenetic relationships in ten Drosophila species of the annulimana group (Diptera, Drosophilidae)
Polytene chromosomes banding patterns of ten of the 16 species of the Neotropical annulimana group of Drosophila were used to propose phylogenetic relationships among species. Drosophila annulimana chromosomes were used as the standard sequence and the most parsimonious series of changes (paracentric inversions) were considered. In some cases, intermediate h
Genetics and Molecular Biology. Publicado em: 2007
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6. Homology among polytene chromosomes of Zaprionus and Drosophila: photomap and gene mapping by in situ hybridization in Z. indianus / Homologia entre os cromossomos politÃnicos dos gÃneros Drosophila e Zaprionus: fotomapa e mapeamento gÃnico por hibridizaÃÃo in situ em Z. indianu
O drosofilÃdeo Zaprionus indianus invadiu recentemente o Brasil e seu comportamento de praga aumentou o interesse pela sua caracterizaÃÃo genÃtica. A descriÃÃo do cariÃtipo mitÃtico e um mapa desenhado em cÃmara clara do complemento politÃnico foram publicados, porÃm nÃo foi determinada homologia entre os cromossomos dos gÃneros Zaprionus e Dros
Publicado em: 2006
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7. Identificação, caracterização e estudo da expressão dos genes hsc70 e hsp83 em Rhynchosciara americana / Identification, characterization and study of expression of the genes hsc70 and hsp83 in Rhynchosciara americana
With the idea of identify some of these proteins involved in the folding process of the proteins synthesized on the Rhynchosciara salivary gland, this project started adopting the shotgun cDNA sequencing strategy. This cDNA library was constructed utilizing salivary glands of Rhynchosciara americana at a developmental period where the cocoon construction beg
Publicado em: 2005
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8. Chromosomal phylogeny of the Drosophila fasciola species subgroup revisited (Diptera, Drosophilidae)
The analysis of polytene chromosomes in 26 strains of seven species in the Drosophila fasciola subgroup, from several locations in Brazil, in addition to strains of two species belonging to the Drosophila mulleri subgroup (D. aldrichi and D. mulleri), enabled us to determine that the 3c inversion found in the latter species differ in one of its break points
Genetics and Molecular Biology. Publicado em: 2004
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9. Sequencing and analysis of a EST Bank from salivary glands of Rhynchosciara americana and characterization of the gene RaDup / Sequenciamento e análise de um banco de cDNA de glândulas salivares de Rhynchosciara americana e caracterização do gene RaDup
In this work EST sequencing was used as strategy to find messages related to development, metabolism and polyteny/amplification in salivary glands of Rhynchosciara americana, a dipteran (Sciaridae) that shows in this tissue giant polytene chromosomes and gene amplification tightly regulated throughout development of the larvae. A total of 8193 EST sequences
Publicado em: 2004
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10. Malpighian tubule polytene chromosomes of Culex quinquefasciatus (Diptera, Culicinae)
Dipteran polytene chromosomes provide an excellent model for understanding in species complexes, as well as for structural and functional cytogenetics. The status of species in the Culex pipiens complex is controversial and the use of polytene chromosomes for cytogenetic analysis in the subfamily Culicinae has been difficult because of methodological problem
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-04
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11. A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)
Polytene chromosome preparations were obtained from larval, pupal and adult female Malpighian tubules of Aedes aegypti. The Malpighian tubules of the pupae (0-4 h old) from larvae reared at 20ºC provided the best cytogenetic analysis. The interaction of nucleic acids and proteins that influence the spreading of the chromosomes could be reduced with the prep
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-04
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12. Isozyme variation in four species of the Simulium perflavum species group (Diptera: Simuliidae) from the Brazilian Amazon
Electrophoretic studies of isozymes were done with four closely related species of the Simulium perflavum species group (Diptera: Simuliidade) in the Brazilian Amazon, using last-instar larvae collected in the field. Ten enzymes were studied, which yielded 11 loci. Diagnostic loci were not found between Simulium maroniense cytotype D and Simulium rorotaense.
Genetics and Molecular Biology. Publicado em: 2003