Proenzyme
Mostrando 1-12 de 88 artigos, teses e dissertações.
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1. Structural and functional studies about Xylellain, the cysteine protease from bacterium / Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa
A Xylella fastidiosa é uma bactéria gram-negativa que infecta o xilema das plantas causando muitas vezes a maturação precoce e a diminuição dos frutos. Ela é responsável por importantes perdas na economia, no Brasil é a causadora de doenças de Citrus Variegated Chlorosis (CVC) e a da doença de Pierce nos Estados Unidos. As proteases desempenham fu
Publicado em: 2008
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2. Role of gelatinases MMP-2 and MMP-9 in tissue remodeling following acute lung injury
Acute lung injury is characterized by a severe disruption of alveolo-capillary structures and includes a variety of changes in lung cell populations. Evidence suggests the occurrence of rupture of the basement membranes and interstitial matrix remodeling during acute lung injury. The dynamic equilibrium of the extracellular matrix (ECM) under physiological c
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
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3. Conversion of prohistidine decarboxylase to histidine decarboxylase: Peptide chain cleavage by nonhydrolytic serinolysis
Unlabeled prohistidine decarboxylase and prohistidine decarboxylase containing L-[carboxyl-18O]serine or L-[hydroxyl-18O]serine were isolated in homogeneous form from mutant 3 of Lactobacillus 30a grown with the appropriately labeled serine. There was no randomization or redistribution of label during growth, isolation of the protein, or enzymatic hydrolysis
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4. Mutational Analysis of the Drosophila Snake Protease: An Essential Role for Domains within the Proenzyme Polypeptide Chain
Two genes involved in the generation of dorsoventral asymmetry in the developing Drosophila melanogaster embryo, snake and easter, encode the zymogen form of serine proteases. Mutant alleles of snake were cloned and sequenced revealing two types of lesions: point mutations which alter the amino acid sequence (snk(073) and snk(rm4)) and point mutations which
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5. Role of NifS in maturation of glutamine phosphoribosylpyrophosphate amidotransferase.
Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11-residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature e
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6. Novel Regulation of Type IV Collagenase (Matrix Metalloproteinase-9 and -2) Activities by Transforming Growth Factor-β1 in Human Prostate Cancer Cell Lines
The type IV collagenases/gelatinases matrix metalloproteinase-2 (MMP-2) and MMP-9 play a variety of important roles in both physiological and pathological processes and are regulated by various growth factors, including transforming growth factor-β1 (TGF-β1), in several cell types. Previous studies have suggested that cellular control of one or both collag
The American Society for Cell Biology.
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7. Amine cations promote concurrent conversion of prohistidine decarboxylase from Lactobacillus 30a to active enzyme and a modified proenzyme
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8. Human fibroblast collagenase: glycosylation and tissue-specific levels of enzyme synthesis.
Human skin fibroblasts secrete collagenase as two proenzyme forms (57 and 52 kDa). The minor (57-kDa) proenzyme form is the result of a partial posttranslational modification of the major (52-kDa) proenzyme through the addition of N-linked complex oligosaccharides. Human endothelial cells as well as fibroblasts from human colon, cornea, gingiva, and lung als
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9. Maintenance of caspase-3 proenzyme dormancy by an intrinsic “safety catch” regulatory tripeptide
Caspase-3 is synthesized as a dormant proenzyme and is maintained in an inactive conformation by an Asp-Asp-Asp “safety-catch” regulatory tripeptide contained within a flexible loop near the large-subunit/small-subunit junction. Removal of this “safety catch” results in substantially enhanced autocatalytic maturation as well as increased vulnera
The National Academy of Sciences.
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10. Amine cations promote concurrent conversion of prohistidine decarboxylase from Lactobacillus 30a to active enzyme and a modified proenzyme.
Activation of prohistidine decarboxylase (pi 6) from Lactobacillus 30a proceeds by an intramolecular, pH- and monovalent cation-dependent reaction in which its constituent pi chains are cleaved nonhydrolytically between Ser-81 and Ser-82 with loss of NH3 and conversion of Ser-82 to the pyruvoyl residue of active histidine decarboxylase (alpha beta)6. Amines
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11. Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.
A carboxyl-terminal chymotryptic peptide from mature human placental alkaline phosphatase was purified by HPLC and monitored by a specific RIA. Sequencing and amino acid assay showed that the carboxyl terminus of the peptide was aspartic acid, representing residue 484 of the proenzyme as deduced from the corresponding cDNA. Further analysis of the peptide sh
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12. Structure-activity relationships of an exotoxin of Pseudomonas aeruginosa.
The relation of the structure of Pseudomonas aeruginosa exotoxin A (PA toxin) to its enzymatic activity (adenosine 5'-diphosphate-ribosyl transferase) in vitro and to its toxicity in vivo was examined. PA toxin is produced as a single polypeptide chain with a molecular weight of about 71,500. PA toxin is produced by Pseudomonas as a toxic proenzyme that lack