Proline Rich Antigen 2
Mostrando 1-12 de 32 artigos, teses e dissertações.
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1. Caracterização de um Antígeno Rico em Prolina(PRA/Ag2) do fungo patogênico humano Paracoccidioides brasiliensis / Characterization of a Proline-Rich Antigen (PRA/Ag2) of the human pathogenic fungus Paracoccidioides brasiliensis
O fungo dimórfico Paracoccidioides brasiliensis é o agente causador da micose sistêmica mais prevalente na América Latina. Em humanos, a infecção se dá pela inalação de propágulos do fungo, que ao alcançarem o epitélio pulmonar transformam se na fase leveduriforme, parasitária, do fungo P. brasiliensis. Neste trabalho,descrevemos a caracterizaç
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 31/01/2008
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2. Caracterização de dois genes contíguos de Trypanosoma cruzi que codificam antígenos com repetições de epitopos imunodominantes / Characterization of two contiguous antigen genes of Trypanosoma cruzi presenting immunodominant repetitive domains
Characterization of two contiguous antigen genes of Trypanosoma cruzi presenting immunodominant repetitive epitopes A genomic library of T. cruzi in the vector λgt11 was screened with antitrypomastigote serum. Two clones, named B12 and B13, were isolated and shown to express proteins which contain tandemly repeated units of 20 amino acids (B12) and 12 a
Publicado em: 1994
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3. The Tec Family Kinase Itk Exists as a Folded Monomer in Vivo*
Tec family tyrosine kinases transduce signals from antigen and other receptors. In particular, Itk plays an important role in T-cell development and activation. Itk has an N-terminal pleckstrin homology domain, a Tec Homology domain with a proline-rich region, SH3 and SH2 domains and a kinase domain, the structure each of which has been determined. However,
American Society for Biochemistry and Molecular Biology.
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4. Promoter-specific transactivation of hepatitis B virus transcription by a glutamine- and proline-rich domain of hepatocyte nuclear factor 1.
The cloned transcription factor hepatocyte nuclear factor 1 (HNF1) transactivates transcription from the hepatitis B virus (HBV) large surface antigen promoter but does not influence the transcriptional activities of the other three HBV promoters. This indicates that this transcription factor can differentially influence the activities of the HBV promoter. B
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5. PCR Assays for Identification of Coccidioides posadasii Based on the Nucleotide Sequence of the Antigen 2/Proline-Rich Antigen
A conventional nested PCR and a real-time LightCycler PCR assay for detection of Coccidioides posadasii DNA were designed and tested in 120 clinical strains. These had been isolated from 114 patients within 10 years in Monterrey, Nuevo Leon, Mexico, known to be endemic for coccidioidomycosis. The gene encoding the specific antigen 2/proline-rich antigen (Ag2
American Society for Microbiology.
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6. Retrovirus Targeting by Tropism Restriction to Melanoma Cells
Targeted vectors will be necessary for many gene therapy applications. To target retroviruses to melanomas, we fused a single-chain variable fragment antibody (scFv) directed against the surface glycoprotein high-molecular-weight melanoma-associated antigen (HMW-MAA) to the amphotropic murine leukemia virus envelope. A proline-rich hinge and matrix metallopr
American Society for Microbiology.
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7. MTC28, a novel 28-kilodalton proline-rich secreted antigen specific for the Mycobacterium tuberculosis complex.
Proteins that are actively secreted by Mycobacterium tuberculosis serve as major targets of immune responses in the infected host. To identify and purify novel proteins in the filtrates of M. tuberculosis cultures, a bacteriophage lambda library of M. tuberculosis H37Rv DNA was immunoscreened by using an anti-culture filtrate rabbit antiserum. Of 20 positive
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8. Resistance to Coccidioides immitis in Mice after Immunization with Recombinant Protein or a DNA Vaccine of a Proline-Rich Antigen
Two inbred strains of mice (BALB/c and C57BL/6) were vaccinated with either recombinant expression protein of a Coccidioides immitis spherule-derived proline-rich antigen (rPRA) in monophosphoryl lipid A-oil emulsion adjuvant or a DNA vaccine based on the same antigen. Four weeks after vaccination, mice were infected intraperitoneally with arthroconidia. By
American Society for Microbiology.
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9. Role of the Proline-Rich Motif of Bovine Leukemia Virus Transmembrane Protein gp30 in Viral Load and Pathogenicity in Sheep
The cytoplasmic tail of bovine leukemia virus (BLV) transmembrane protein gp30 has multiple amino acid motifs that mimic those present in signaling proteins associated with B-cell and T-cell receptors. The proline-rich motif of gp30, PX2PX4–5P, is analogous to the recognition site of Src homology 3 (SH3) domains of signaling molecules. Using site-directed
American Society for Microbiology.
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10. Localization within a Proline-Rich Antigen (Ag2/PRA) of Protective Antigenicity against Infection with Coccidioides immitis in Mice
Subunits of a proline-rich coccidioidal antigen (Ag2/PRA) of Coccidioides immitis were analyzed by comparison as vaccines in mice. The optimal dose of plasmid vaccine encoding full-length Ag2/PRA was determined to be between 10 and 100 μg. Mice vaccinated with plasmids encoding amino acids (aa) 1 to 106 were as protective as full-length Ag2/PRA (aa 1 to 194
American Society for Microbiology.
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11. The Interaction of Activated Integrin Lymphocyte Function-associated Antigen 1 with Ligand Intercellular Adhesion Molecule 1 Induces Activation and Redistribution of Focal Adhesion Kinase and Proline-rich Tyrosine Kinase 2 in T Lymphocytes
Integrin receptors play a central role in the biology of lymphocytes, mediating crucial functional aspects of these cells, including adhesion, activation, polarization, migration, and signaling. Here we report that induction of activation of the β2-integrin lymphocyte function-associated antigen 1 (LFA-1) in T lymphocytes with divalent cations, phorbol este
The American Society for Cell Biology.
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12. An immunoreactive apoglycoprotein purified from Coccidioides immitis.
Deglycosylation of glycoproteins in a lysate of spherules of Coccidioides immitis has permitted purification and partial characterization of a proline-rich pronase-sensitive antigen. Moreover, soluble antigen specifically stimulated lymphocytes from persons with dermal delayed-type hypersensitivity to coccidioidal antigens. When related to reference coccidio