Pronuclear Transfer
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle.
Ooplasmic factors drive nuclear organization after fertilization and are also important for re-programming in nuclear transfer procedures, in which artificial activation is essential for reconstructed embryos to progress in development. The present research evaluated the effect of pronuclear transfer (PT) between zygotes parthenogenetically activated with io
Animal Reproduction Science. Publicado em: 2011
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2. O papel do pronuclear (1976 1986) na formação de recusos humanos para a área nuclear no Brasil
In 1975, an agreement between the governments of Brazil and West-Germany was signed for the construction of nuclear power plants in Brazil as well as the extraction, processing and enrichment of uranium. As the result of the first oil crisis and facing the perspective of a continuous world energy crisis, the Brazilian government decided to turn to nuclear po
Publicado em: 2006
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3. Generation of bovine transgenics using somatic cell nuclear transfer
The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have either been inefficient or not available in all animals, bovine included. More recently somatic cell nuclear
BioMed Central.
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4. Episomal maintenance of a bovine papilloma virus vector in transgenic mice.
We have used a bovine papillomavirus-based vector to generate transgenic mice. Transgenic mice result from either pronuclear or cytoplasmic injections of the vector into fertilized eggs. Of 30 mice generated by microinjection, 27 (90%) contained the vector in its episomal form, at less than one copy per cell. This represents a highly efficient means of gene
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5. Microsurgically produced homozygous-diploid uniparental mice
Shortly after fertilization, either the male or the female pronucleus was microsurgically removed from 202 F1 hybrid eggs derived from crosses of two inbred strains. Subsequent incubation of these haploid eggs in medium containing cytochalasin B, which inhibits cytokinesis but not nuclear division, enabled the remaining pronucleus to become diploid. After nu