Protein Processing Post Translational
Mostrando 1-12 de 76 artigos, teses e dissertações.
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1. Functional redundancy in the post-translational processing of the vitellogenin VIT-6 precursor by Caenorhabditis elegans proprotein convertases. / Evidências de redundância funcional entre as pró-hormônio convertases no processamento pós-traducional do precursor da vitelogenina VIT-6 do nematóide Caenorhabditis elegans.
Caenorhabditis elegans possui quatro genes de kpcs (kex2/subtilisin-like proprotein convertases): kpc-1, kpc-2/egl-3, kpc-3/aex-5, kpc-4/bli-4. Em C. elegans, dois dos quatro polipeptídeos de vitelogenina encontrados dentro dos ovócitos, YP115 e YP88, se originam a partir de um precursor polipeptídico (VIT-6) clivado pós-traducionalmente após o motivo R
Publicado em: 2009
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2. Heterologous expression and biochemical characterization of an α1,2-mannosidase encoded by the Candida albicans MNS1 gene
Protein glycosylation pathways, commonly found in fungal pathogens, offer an attractive new area of study for the discovery of antifungal targets. In particular, these post-translational modifications are required for virulence and proper cell wall assembly in Candida albicans, an opportunistic human pathogen. The C. albicans MNS1 gene is predicted to encode
Memórias do Instituto Oswaldo Cruz. Publicado em: 2008-11
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3. Identification of proteins from honeybee venom (Apis mellifera L.) immunoreactives to antivenom serum through a proteomic approach / Identificação das proteínas do veneno de abelhas africanizadas (Apis mellifera L.) imunoreativas ao soro antiveneno por abordagem proteômica
O estudo de venenos de artrópodes é de grande interesse para melhorar os tratamentos contra envenenamentos e oferece uma ótima ferramenta para melhor compreensão dos sistemas nervoso e imunológico, coagulação sanguínea e respostas inflamatórias. As abelhas são um dos animais venenosos mais estudados e a elucidação do seu proteoma é de interesse
Publicado em: 2008
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4. Caracterização da expressão e função de receptores recombinantes do hormônio folículo-estimulante. / Characterization of expression and function of recombinant follicle-stimulating receptors.
The follicle stimulating hormone receptor (FSHR) is a member of a family of Gprotein-coupled receptors that has a large aminoterminal domain, rich in leucine repeats. The FSHR, together with the luteinizing hormone receptor, controls gonadal function in vertebrates. Mutations in the FSHR can alter its binding and signaling properties, and drastically affect
Publicado em: 2006
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5. Role of propeptide glycan in post-translational processing and transport of barley lectin to vacuoles in transgenic tobacco.
Mature barley lectin is a dimeric protein composed of two identical 18-kilodalton polypeptides. The subunits of barley lectin are initially synthesized as glycosylated proproteins, which are post-translationally processed to the mature protein preceding or concomitant with deposition of barley lectin in vacuoles. To investigate the functional role of the gly
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6. Processing of alkaline phosphatase precursor to the mature enzyme by an Escherichia coli inner membrane preparation.
An inner membrane preparation co-translationally cleaved both the alkaline phosphatase and bacteriophage f1 coat protein precursors to the mature proteins. Post-translational outer membrane proteolysis of pre-alkaline phosphatase generated a protein smaller than the authentic monomer.
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7. In Vivo Stable Isotope Labeling of Fruit Flies Reveals Post-transcriptional Regulation in the Maternal-to-zygotic Transition*
An important hallmark in embryonic development is characterized by the maternal-to-zygotic transition (MZT) where zygotic transcription is activated by a maternally controlled environment. Post-transcriptional and translational regulation is critical for this transition and has been investigated in considerable detail at the gene level. We used a proteomics
The American Society for Biochemistry and Molecular Biology.
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8. Non-glycosylated recombinant pro-concanavalin A is active without polypeptide cleavage.
The complex post-translational processing of concanavalin A (Con A) in maturing jackbeans is unique because the non-glycosylated mature active protein is circularly permuted in primary sequence relative to its own inactive precursor (glycosylated pro-Con A) and to other legume lectins. We show here that non-glycosylated pro-Con A expressed in bacteria from r
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9. Characterization of an endopeptidase involved in pre-protein processing.
Proteolytic removal of the pre-segment from growing nascent chains of pre-human placental lactogen (hPL) occurred during in vitro translation of placental mRNA if crude membranes derived from ascites lysates, dog pancreas, or rat liver rough endoplasmic reticulum were added to the translation mixtures. The cotranslational proteolytic event was inhibited by t
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10. New mechanism for post-translational processing during assembly of a cytoplasmic membrane protein?
Insertion of nitrate reductase into the Escherichia coli cytoplasmic membrane was examined by following the fate of pulse-labeled enzyme in both the membrane and cytoplasm during various times after the addition of an unlabeled chase. The polypeptide composition of this labeled enzyme was determined by autoradiography of immunoprecipitated material after sep
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11. Nonstructural proteins of Semliki Forest virus: synthesis, processing, and stability in infected cells.
The synthesis of the nonstructural (ns) proteins of Semliki Forest virus was studied in vivo. The fourth ns protein, ns60, was identified and isolated. The order of translation (NH2-ns70-ns86-ns60-ns72-COOH) was determined by using various labeling procedures after or in the presence of a hypertonic block of translation initiation. A sequential labeling proc
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12. The Ty transposon of Saccharomyces cerevisiae determines the synthesis of at least three proteins.
Two new Ty determined proteins have been identified by placing a Ty transcriptional unit under the control of a high efficiency yeast expression vector. One of these proteins is the product of a post-translational processing event and it binds nucleic acids. A previously identified protein, pl (Tyl-15), has also been shown to bind nucleic acids and to be mod