Proteins Separation
Mostrando 1-12 de 635 artigos, teses e dissertações.
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1. Protein PEGylation for the design of biobetters: from reaction to purification processes
The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and
Braz. J. Pharm. Sci.. Publicado em: 08/11/2018
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2. Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis
Abstract Alveolar bone healing after upper incisor extraction in rats is a classical model of preclinical studies. The underlying morphometric, cellular and molecular mechanism, however, remains imprecise in a unique study. Objectives The aim of this study was therefore to characterize the alveolar bone healing after upper incisor extraction in rats by mic
J. Appl. Oral Sci.. Publicado em: 18/06/2018
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3. DEVELOPMENT OF A NEW PROCESS FOR PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM Streptococcus pneumoniae SEROTYPE 14
Abstract The main virulence factor of Streptococcus pneumoniae is the capsular polysaccharide (PS), which is the antigen of all current vaccines that are prepared with PS purified from serotypes prevalent in the population. In this work, three purification strategies were evaluated and a new process was developed for purification of serotype 14 PS (PS14), re
Braz. J. Chem. Eng.. Publicado em: 2016-09
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4. Evolutionary analysis of apolipoprotein E by Maximum Likelihood and complex network methods
Abstract Apolipoprotein E (apo E) is a human glycoprotein with 299 amino acids, and it is a major component of very low density lipoproteins (VLDL) and a group of high-density lipoproteins (HDL). Phylogenetic studies are important to clarify how various apo E proteins are related in groups of organisms and whether they evolved from a common ancestor. Here, w
Genet. Mol. Biol.. Publicado em: 14/07/2016
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5. Sustainable Management of Keratin Waste Biomass: Applications and Future Perspectives
Keratin is a durable, fibrous protein which is mainly present in higher vertebrates (mammals, birds and reptiles) and humans epithelial cells. Food industry especially the meat market, slaughter house and wool industry produces million of tons of keratin containing biomass. These industries are constantly growing and the major producers include USA, Brazil a
Braz. arch. biol. technol.. Publicado em: 29/04/2016
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6. CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns o
Quím. Nova. Publicado em: 2016-02
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7. DNA damage in the kidney tissue cells of the fish Rhamdia quelen after trophic contamination with aluminum sulfate
Abstract Even though aluminum is the third most common element present in the earth's crust, information regarding its toxicity remains scarce. It is known that in certain cases, aluminum is neurotoxic, but its effect in other tissues is unknown. The aim of this work was to analyze the genotoxic potential of aluminum sulfate in kidney tissue of the fish Rham
Genet. Mol. Biol.. Publicado em: 24/11/2015
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8. Composição química dos grãos e da cera foliar de variedade Glycine max (L.) Merrill cv. MG/ BR-46 Conquista cultivada sob atmosfera enriquecida de gás carbônico e temperatura elevada
The concentration of carbon dioxide (CO2) has increased in the last decades, due mainly to the combustion of fossil fuels. A gradual increase of the global temperature is expected as a consequence. Soybean is an important crop in most countries, being consumed either as human or domestic animals food. The present work is composed of two chapters, the first d
Publicado em: 07/02/2014
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9. Separomics applied to the proteomics and peptidomics of low-abundance proteins: choice of methods and challenges - a review
The enrichment and isolation of proteins are considered limiting steps in proteomic studies. Identification of proteins whose expression is transient, those that are of low-abundance, and of natural peptides not described in databases, is still a great challenge. Plant extracts are in general complex, and contaminants interfere with the identification of pro
Genetics and Molecular Biology. Publicado em: 2012
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10. Fracionamento de cobre em proteínas do plasma, músculo e fígado de tilápia do Nilo
Copper fractionation in plasma, muscle and liver of Nile tilapia was performed after protein separation by 2D-PAGE. SR XRF analysis indicated the presence of copper in three protein spots of plasma, and in two protein spots of muscle and liver, respectively. Copper ions were found to be distributed mostly in proteins that had a molar mass of less than 54 kDa
Química Nova. Publicado em: 2012
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11. Method optimization for proteomic analysis of soybean leaf: improvements in identification of new and low-abundance proteins
The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the existence of published results for the optimization of proteomic
Genetics and Molecular Biology. Publicado em: 2012
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12. Desenvolvimento de um adsorvente contínuo supermacroporoso de troca catiônica para recuperação de lactoferrina de soro de leite / Development of a cation exchange solid supermacroporous adsorbent for lactoferrin recovery from whey
Whey proteins have functional, nutritional and technological properties promoting their use as ingredients and supplements. Sweet whey obtained from Minas Frescal cheese production and concentrated by ultrafiltration was used in the lactoferrin capture in a supermacroporous adsorbent. Supermacroporous monolithic columns have been prepared by radical cryo-cop
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/11/2011