Pst Operon
Mostrando 1-12 de 48 artigos, teses e dissertações.
-
1. Estudo do papel do sistema de captação de fosfato inorgânico (Pst) na fisiologia e patogênese de Streptococcus mutans. / Study of the role of inorganic phosphate uptake system (Pst) in the physiology and pathogenesis of Streptococcus mutans.
O fosfato inorgânico é um composto essencial por estar relacionado com diversos processos metabólicos e biossíntese de moléculas relevantes à sobrevivência celular. Em bactéria são conhecidos dois tipos principais de transportadores específicos de fosfato inorgânico, o sistema Pit, de baixa afinidade, e o sistema Pst de alta afinidade, um ABC tran
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/01/2011
-
2. Regulation of enteropathogenic Escherichia coli (EPEC) adhesion by genes related to nutrional shortage and stress. / Regulação da adesão de Escherichia coli enteropatogênica (EPEC) por genes de resposta à limitação nutricional e estresse.
Enteropathogenic E. coli (EPEC) is one of the causes of diarrhea in children. Phosphate (Pi) shortage induces transcription of the genes known as the PHO regulon. These genes are controlled by the Pst system, that is also a high-affinity Pi transporter, and represses PHO expression under Pi-replete conditions. PHO is also controlled by the two-component syst
Publicado em: 2009
-
3. Análise transcricional do operon pst de Escherichia coli. / Transcriptional analisys of pst operon Escherichia coli.
O operon pst de Escherichia coli é formado pelos genes pstS, pstC, pstA, pstB e phoU. Os quatro primeiros genes codificam proteínas que compõem um sistema de transporte do tipo ABC denominado Pst. O sistema Pst, junto com a proteína PhoU participa da repressão dos genes do regulon PHO. A transcrição dos genes do operon pst é induzida pela carência d
Publicado em: 2007
-
4. Identification of a mutation in the pst-phoU operon that reduces pathogenicity of an Escherichia coli strain causing septicemia in pigs.
We used transposon (TnphoA) mutagenesis to study the role of virulence factors of pathogenic Escherichia coli strains associated with septicemia in calves and piglets. We have produced an avirulent and serum-sensitive mutant of wild-type pathogenic strain 5131 O115:K"V165":F165 and have localized and identified the TnphoA insertion in the pstC gene of the ps
-
5. Genetic analysis of mutants affected in the Pst inorganic phosphate transport system.
A number of mutant alleles affecting the Pst phosphate transport system have been divided into three complementation groups on the basis of constitutive alkaline phosphatase activity in appropriate partial diploid strains. The three complementation groups were represented by the alleles pstA2 and phoT32 and the newly described allele pstB401. The two alleles
-
6. The pst Operon of Bacillus subtilis Is Specifically Induced by Alkali Stress
To cope with a sudden increase in the external pH value to 8.9, Bacillus subtilis cells induce about 80 genes which can be divided into two classes. Most of these genes are members of the σW regulon, while some are under the control of so-far-unknown transcriptional regulators. The genes of the pst operon belong to the second class. Here, we attempted to an
American Society for Microbiology.
-
7. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
The phosphate regulon is negatively regulated by the PstSCAB transporter and PhoU protein by a mechanism that may involve protein-protein interaction(s) between them and the Pi sensor protein, PhoR. In order to study such presumed interaction(s), mutants with defined deletions of the pstSCAB-phoU operon were made. This was done by construction of M13 recombi
-
8. The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.
Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promoter contains structural features analogous
-
9. Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.
The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kinase (ppk), acetate kinase (ackA), and the
-
10. Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides light-harvesting B800-850-alpha and B800-850-beta genes.
Two deoxyoligonucleotide probes were synthesized in accordance with the available amino acid sequence of the B800-850-beta polypeptide from Rhodobacter sphaeroides and were used to isolate a 2.6-kilobase PstI fragment from R. sphaeroides 2.4.1 chromosomal DNA. Identification of the B800-850-beta and B800-850-alpha structural genes, pucB and pucA, was confirm
-
11. Identification of a Regulated Alkaline Phosphatase, a Cell Surface-Associated Lipoprotein, in Mycobacterium smegmatis
Although alkaline phosphatases are common in a wide variety of bacteria, there has been no prior evidence for alkaline phosphatases in Mycobacterium smegmatis. Here we report that transposon insertions in the pst operon, encoding homologues of an inorganic phosphate transporter, leads to constitutive expression of a protein with alkaline phosphatase activity
American Society for Microbiology.
-
12. Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS.
The xylDEGF operon and the regulatory gene xylS of the TOL plasmid found in Pseudomonas putida mt-2 were cloned onto Escherichia coli vector plasmids. A 9.5-kilobase fragment, derived from the TOL segment of pTN2 deoxyribonucleic acid, carried the xyl genes D, E, G, and F, which encode toluate oxygenase, catechol 2,3-oxygenase, 2-hydroxymuconic semialdehyde