Pyrococcus
Mostrando 1-12 de 200 artigos, teses e dissertações.
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1. Clonagem, expressão e produção de dna polimerase de alta fidelidade de pyrococcus furiosus
A reação em cadeia da DNA polimerase (PCR) é uma das técnicas de biologia molecular mais importantes da atualidade e amplamente utilizada em laboratórios clínicos e de pesquisa. Considerando a alta fidelidade requerida para algumas PCRs e o alto custo para a aquisição de DNA polimerases termoestáveis de alta fidelidade, o objetivo que norteou o dese
Publicado em: 2010
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2. Strutural and functional analysis of the SBDS protein family / Estudos estruturais e funcionais de proteinas da familia SBDS com enfase nas ortologas de Trypanosoma cruzi e humana
Proteínas da família SBOS (Shwachman-Bodian-Diamond Syndrome) ocorrem largamente na natureza e s.ão bastante conservadas, apresentando ortólogas em Archaea e eucariotos. Estudos de análises genômica e biofísica tem relacionado a SBOS com o metabolismo de RNA e biosíntese de ribossomos. O gene ortólogo da SBOS de Archaea está localizado em um operon
Publicado em: 2009
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3. Caracterização estrutural e bioquimica da hipoxantina-guanina-xantina fosforribosiltransferase / Biochemical and structural characterization of the hypoxanthine-guanine-xantina phosphoribosyltransferase
Os genes que codificam para a 6-oxopurina fosforribosiltransferase (HPRT, EC2.4.2.8) dos organismos Pyrococcus horikoshii e Schistosoma mansoni foram clonados em vetores de expressão. As proteínas foram expressas e purificadas em larga escala no sistema de expressão de Escherichia coli. Estudos cinéticos mostraram que a enzima de P. horikoshii é capaz d
Publicado em: 2008
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4. Estudo funcional e estrutural de Nip7p, uma proteina conservada envolvida na sintese de ribossomos / Functional and structural analysis of Nip7p, a conserved protein involved in ribosome biogenesis
A síntese de ribossomos é um processo conservado em eucariotos e se inicia com a transcrição dos rRNAs no nucléolo. Mais de 170 fatores atuam de forma transitória no processamento dos precursores para gerar os rRNAs maduros que formarão as subunidades ribossomais no citoplasma. Entre as proteínas envolvidas na síntese de ribossomos está a Nip7p, um
Publicado em: 2007
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5. Análise estrutural e funcional de cofatores do exossomo em Saccharomyces cerevisiae e Pyrococcus / Structural and functional analysis of exosome cofactors in Saccharomyces cerevisiae and Pyrococcus
A síntese ribossomal é uma das maiores atividades em células eucarióticas. Este processo inicia-se no nucléolo e é finalizado após a exportação das subunidades 40S e 60S para o citoplasma. Três dos RNAs ribossomais de eucariotos (18S, 5.8S e 25S) são sintetizados como um transcrito primário de 35S, o qual é processado através de uma complexa e
Publicado em: 2006
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6. A cell-free transcription system for the hyperthermophilic archaeon Pyrococcus furiosus.
We describe here the establishment of a cell-free transcription system for the hyperthermophilic Archaeon Pyrococcus furiosus using the cloned glutamate dehydrogenase (gdh) gene as template. The in vitro system that operated up to a temperature of 85 degrees C initiated transcription 23 bp downstream of a TATA box located 45 bp upstream of the translational
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7. Genome Evolution at the Genus Level: Comparison of Three Complete Genomes of Hyperthermophilic Archaea
We have compared three complete genomes of closely related hyperthermophilic species of Archaea belonging to the Pyrococcus genus: Pyrococcus abyssi, Pyrococcus horikoshii, and Pyrococcus furiosus. At the genomic level, the comparison reveals a differential conservation among four regions of the Pyrococcus chromosomes correlated with the location of genetic
Cold Spring Harbor Laboratory Press.
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8. Hyperthermostable Endoglucanase from Pyrococcus horikoshii
An endoglucanase homolog from the hyperthermophilic archaeon Pyrococcus horikoshii was expressed in Escherichia coli, and its enzymatic characteristics were examined. The expressed protein was a hyperthermostable endoglucanase which hydrolyzes celluloses, including Avicel and carboxymethyl cellulose, as well as β-glucose oligomers. This enzyme is the first
American Society for Microbiology.
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9. Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-Å resolution
The intracellular protease from Pyrococcus horikoshii (PH1704) and PfpI from Pyrococcus furiosus are members of a class of intracellular proteases that have no sequence homology to any other known protease family. We report the crystal structure of PH1704 at 2.0-Å resolution. The protease is tentatively identified as a cysteine protease based on the p
The National Academy of Sciences.
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10. Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes
The genus Thermococcus, comprised of sulfur-reducing hyperthermophilic archaea, belongs to the order Thermococcales in Euryarchaeota along with the closely related genus Pyrococcus. The members of Thermococcus are ubiquitously present in natural high-temperature environments, and are therefore considered to play a major role in the ecology and metabolic acti
Cold Spring Harbor Laboratory Press.
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11. Cultivation Techniques for Hyperthermophilic Archaebacteria: Continuous Culture of Pyrococcus furiosus at Temperatures near 100°C
A system which allows continuous cultivation of hyperthermophilic archaebacteria at temperatures approaching 100°C has been developed. Continuous cultivation of the hyperthermophile Pyrococcus furiosus was carried out with this system; the resulting dilution rate and gas production profiles are discussed.
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12. Physiological Responses of the Hyperthermophilic Archaeon “Pyrococcus abyssi” to DNA Damage Caused by Ionizing Radiation
The mechanisms by which hyperthermophilic Archaea, such as “Pyrococcus abyssi” and Pyrococcus furiosus, survive high doses of ionizing gamma irradiation are not thoroughly elucidated. Following gamma-ray irradiation at 2,500 Gy, the restoration of “P. abyssi” chromosomes took place within chromosome fragmentation. DNA synthesis in irradiated “P. ab
American Society for Microbiology.