Rapid Methods Microbiology
Mostrando 1-12 de 70 artigos, teses e dissertações.
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1. Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis
Abstract Objective The diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB64 to detect Mycobacterium tuberculosis. Methods 150 ex
Braz J Infect Dis. Publicado em: 2016-06
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2. Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection
Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable t
Braz. J. Microbiol.. Publicado em: 25/07/2014
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3. Avaliação da técnica 3Mtm Petrifilm tm para análises microbiológicas em água de consumo humano na região de Campinas / Evaluation of 3Mtm Petrifilm tm technique for human drink water analysis in Campinas region
No Brasil, a Portaria MS nº. 518/2004 do Ministério da Saúde estabelece, entre outros parâmetros, a análise de coliformes totais, termotolerantes ou Escherichia coli (E. coli) e de bactérias heterotróficos para análise de água para consumo humano, em toda e qualquer situação, incluindo fontes individuais como poços, minas e nascentes. A Agência
Publicado em: 2011
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4. Uso de microorganismos promotores de crescimento como tecnologia para incrementar a produção de madeira e sequestro de carbono de Schizolobium amazonicum Huber ex Ducke (Paricá) em condições de campo
Schizolobium amazonicum Heber ex Ducke occurs naturally in Amazon and presents huge commercial importance due to its rapid growth and excellent performance on cropping systems a fact that has been raising much interest in the study of new strategies to increase more efficiently the wood yield. The use of soil microorganisms and its growth-promoting potent
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 22/04/2010
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5. Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units
The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR
Brazilian Journal of Infectious Diseases. Publicado em: 2009-08
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6. Estabelecimento de métodos moleculares para aplicação no diagnóstico rápido de virus neurotrópicos. / The integration of molecular methods into the rapid laboratorial diagnostic of neurotropic viruses.
Diversos agentes virais são causadores de meningites e meningoencefalites. Neste estudo, técnicas moleculares foram utilizadas para detecção de HEV, HHV e HAdV em amostras de líquor colhidas de janeiro de 2005 a março de 2007. Dos três métodos de extração de DNA e RNA testados, o kit DNA Qiablood Qiagen® se mostrou o mais sensível e específico.
Publicado em: 2009
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7. Detecção simultânea de Salmonella spp. e Salmonella enteritidis em carcaças de frango por reação em cadeia da polimerase
An increase in human salmonellosis caused by serovar Enteritidis related to consumption of poultry products was observed worldwide in the last years. Safety measures have been adopted to control this serovar. The implementation of Hazard Analysis and Critical Control Points (HACCP) System was not able to eliminate the problem although it has considerably red
Publicado em: 2008
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8. Detection of Salmonella in raw foods of animal origin using Tecra Salmonella VIA and Tecra Salmonella Unique rapid immunoassays and a cultural procedure / Detecção de Salmonella em alimentos crus de origem animal empregando os imunoensaios rápidos TECRATM Salmonella VIA, TECRATM Salmonella UNIQUE e o método convencional de cultura
The presence of Salmonella in 200 raw food samples of animal origin was investigated by means of rapid immunoassays TECRA™ Salmonella VIA and TECRA™ Salmonella UNIQUE (TECRA Diagnostics, Rosewille, NSW, Australia) and the cultural procedure used routinely in Instituto Adolfo Lutz, Sao Paulo, SP. Forty-five samples (22.5%) were Salmonella positive
Publicado em: 2002
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9. Rapid organism identification from Bactec NR blood culture media in a diagnostic microbiology laboratory.
AIMS--To evaluate rapid organism identification on positive blood culture Bactec NR media (phial types 26, 27, 42 and 17), and to assess the usefulness of these procedures in a diagnostic microbiology laboratory. METHODS--Two hundred and sixty, first positive, blood culture bottles from individual patients were tested by rapid identification methods selected
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10. Evaluation of several commercial biochemical and immunologic methods for rapid identification of gram-positive cocci directly from blood cultures.
To develop laboratory methods for the rapid identification of gram-positive cocci from blood cultures, several commercial immunological and biochemical tests for identifying staphylococci and streptococci from two different blood culture systems (Thiol/TSB, Difco Laboratories; BACTEC, Johnston Laboratories, Inc.) were evaluated and compared with conventional
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11. Detection of bacteriuria by automated electrical impedance monitoring in a clinical microbiology laboratory.
An apparatus capable of rapidly detecting changes in electrical impedance was utilized for the continuous monitoring of bacterial growth in routine urine specimens in a clinical laboratory. In a trial study, 200 clinical specimens analyzed by the electrical impedance method resulted in an average detection time of 2.5 h for 41 clinically significant specimen
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12. Rapid, cost-effective identification of group A streptococci and enterococci by pyrrolidonyl-beta-naphthylamide hydrolysis.
Group A streptococci and enterococci were identified by two L-pyrrolidonyl-beta-naphthylamide (PYR) hydrolysis tests. One uses Minitek PYR disks (BBL Microbiology Systems, Cockeysville, Md.); the other uses a swab impregnated with PYR reagent. Both tests and Strep-A-Chek (EY Laboratories, San Mateo, Calif.), a commercially available PYR test, were compared w