Rat Primary Hippocampal Cell Culture
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. Caracterização das vias de morte celular induzida pela metilecgonidina, produto da pirólise da cocaína / Neurotoxicity of anydroecgonine methyl ester, a crack cocaine pyrolysis product
A cocaína é considerada a principal droga de abuso utilizada na América do Sul, sendo que o crack é a via de administração que mais cresceu nos últimos anos. Cabe salientar que o usuário do crack sofre ação tanto da cocaína quanto das substâncias advindas da sua pirólise, dentre elas a metilecgonidina (AEME). Trabalho publicado pelo nosso grupo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/10/2012
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2. Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression.
Clonal cell lines of rat embryonic hippocampal origin have been developed by using retroviral transduction of temperature-sensitive simian virus 40 large tumor antigens. The cell lines undergo morphological differentiation at the nonpermissive temperature and in response to differentiating agents. Immunocytochemical analysis indicates that various lines are
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3. Beta-amyloid neurotoxicity requires fibril formation and is inhibited by congo red.
beta-Amyloid (beta A) is normally produced as a nontoxic soluble peptide. In Alzheimer disease, beta A aggregates and accumulates in the brain as inert diffuse plaques or compact plaques associated with neurodegenerative changes. To determine the relationship of neurotoxicity to the physical state of beta A, we created (i) nonamyloidogenic amorphous aggregat
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4. Calcicludine, a venom peptide of the Kunitz-type protease inhibitor family, is a potent blocker of high-threshold Ca2+ channels with a high affinity for L-type channels in cerebellar granule neurons.
Calcicludine (CaC) is a 60-amino acid polypeptide from the venom of Dendroaspis angusticeps. It is structurally homologous to the Kunitz-type protease inhibitor, to dendrotoxins, which block K+ channels, and to the protease inhibitor domain of the amyloid beta protein that accumulates in Alzheimer disease. Voltage-clamp experiments on a variety of excitable