Recombinants Proteins
Mostrando 1-12 de 323 artigos, teses e dissertações.
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1. Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system
The innovation in industrial process with impact in the efficient production is the major challenge for actual industry. A high numerous of enzymes are utilized in at different level of process; the search for new alternatives with better characteristic has become a field of study of great interest, the recombinant protein achievement in a different host sys
Braz. J. Microbiol.. Publicado em: 20/08/2013
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2. Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell
Brazilian Journal of Microbiology. Publicado em: 2012-03
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3. Determinantes virais envolvidos na adaptabilidade diferencial de dois begomovírus em tomateiro e Nicotiana benthamiana / Viral determinants involves in the differential adaptation of two begomovirus in tomato and Nicotiana benthamiana
Begomoviruses (family Geminiviridae) have a genome composed of a one or two components of circular, single-stranded DNA and infect dicotyledoneous plants. Symptoms induced by the begomovirus Tomato yellow spot virus (ToYSV) in tomato and Nicotiana benthamiana are more severe and appear earlier compared to those induced by Tomato rugose mosaic virus (ToRMV).
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/12/2011
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4. A bovine herpesvirus 5 recombinant defective in the thymidine kinase (TK) gene and a double mutant lacking TK and the glycoprotein E gene are fully attenuated for rabbits
Bovine herpesvirus 5 (BoHV-5), the agent of herpetic meningoencephalitis in cattle, is an important pathogen of cattle in South America and several efforts have been made to produce safer and more effective vaccines. In the present study, we investigated in rabbits the virulence of three recombinant viruses constructed from a neurovirulent Brazilian BoHV-5 s
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-02
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5. FGF2 species of 18 and 22.5 kDa: paracrine molecular signaling and biological functions / FGF2 de 18kDa e de 22,5kDa: sinalização molecular parácrina e funções biológias
FGF2 (Fibroblast Growth Factor 2), the founder of the FGF family, has regulatory functions in mitogenesis, differentiation, morphogenesis and tissue repair. Multiple FGF2 molecular species, sharing a C-terminal sequence of 155 amino acids, are translated from different iniciation sites of the same mRNA. The smaller, the FGF2-18kD, is extracellularly released
Publicado em: 2010
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6. Expressão e reconhecimento imune de alelos conservados de antígenos variantes de Plasmodium falciparum. / Expression and immune recognition of conserved alleles of Plasmodium falciparum variant antigens.
An important factor in the pathogenicity of P. falciparum, the causing agent of malaria is the expression of highly polymorphic antigens encoded by the multigene families var and rif. The extreme polymorphism of these genes in strains from different geographical regions is in contrast with the observation of a number of conserved alleles found in Amazonian i
Publicado em: 2008
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7. Anaplasma marginale : análise da variabilidade do gene msp1 a e avaliação imunogênica da vacina de DNA contendo genes para MSP1a, MSP1b e MSP5 em camundongos BALB/c
Anaplasma marginale is an important tick-transmitted rickettsial pathogen of cattle that invades and multiplies within erythrocytes, causing severe hemolytic anemia during acute infection. Immunization with purified outer membrane proteins (OMP) induces protection against acute A. marginale disease. Of 21 OMP described six major surface proteins (MSPs 1a, 1b
Publicado em: 2007
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8. Desenvolvimento de um modelo de vacinação contra Toxoplasma gondii empregando adenovírus recombinante
In the present study, genes encoding the SAG1, SAG2 and SAG3 proteins of T. gondii were cloned into replication-deficient recombinant adenoviruses, after being modified for appropriate expression in mammalian cells. Modifications included removal of 3-end sequences encoding the GPI-anchoring motifs and, for SAG3, substitution of the 5´-end encoding the natu
Publicado em: 2005
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9. Seqüenciamento e Análise Comparativa do Gene da Proteína Não Estrutural (NS-1) do Vírus Dengue Sorotipo 1 Isolados em Porto Velho-RO
The non structural protein NS1 of the dengue virus still has no clearly defined function in the fhysiopathology of the infection, although there are evidences that it may participate in the early stages of viral replication. The NS1 protein is secreted into the plasma of infected individuals during the early phase of infection and the humoral immune system p
Publicado em: 2005
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10. Avaliação de epitopos na proteina do capsideo de isolados do virus da tristeza dos citros (CTV)
Preliminary studies about the epitopes location in the capside protein of the isolates of the citrus tristeza virus of three isolate (´Pêra IAC , Pêra CB-22 and Pêra CB-104 ) were accomplished. In the first phase, the amplification was accomplished with specific direct and reverse primers of eight different regions of the CP gene, coding for three N-term
Publicado em: 1999
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11. Caracterização de dois genes contíguos de Trypanosoma cruzi que codificam antígenos com repetições de epitopos imunodominantes / Characterization of two contiguous antigen genes of Trypanosoma cruzi presenting immunodominant repetitive domains
Characterization of two contiguous antigen genes of Trypanosoma cruzi presenting immunodominant repetitive epitopes A genomic library of T. cruzi in the vector λgt11 was screened with antitrypomastigote serum. Two clones, named B12 and B13, were isolated and shown to express proteins which contain tandemly repeated units of 20 amino acids (B12) and 12 a
Publicado em: 1994
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12. Expression of Mycobacterium tuberculosis and Mycobacterium leprae proteins by vaccinia virus.
Eight Mycobacterium tuberculosis and M. leprae genes were inserted into the vaccinia virus genome by in vivo recombination. The resulting virus recombinants were shown to express five different M. tuberculosis proteins (71, 65, 35, 19, and 12 kDa) and three M. leprae proteins (65 and 18 kDa and a biotin-binding protein) by Western immunoblot analysis, radioi