Repeated Batch
Mostrando 1-12 de 24 artigos, teses e dissertações.
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1. UTILITY OF FUNCTIONALIZED AGAROSE NANOPARTICLES IN HYDROLYZING LACTOSE IN BATCH REACTORS FOR DAIRY INDUSTRIES
The present study investigates the synthesis of agarose nanoparticles (ANPs) and its surface modification by galactose for the immobilization of β-galactosidase. Galactose modified ANPs retained 91% enzyme activity upon immobilization. Optimum pH (4.5) and temperature (50 ºC) remains unchanged after immobilization. However, immobilized enzyme retained grea
Quím. Nova. Publicado em: 2018-04
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2. Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems
ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Imm
Braz. arch. biol. technol.. Publicado em: 08/01/2018
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3. Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most signi
Braz. arch. biol. technol.. Publicado em: 11/05/2017
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4. Functionalized agarose as an effective and novel matrix for immobilizing Cicer arietinum β-galactosidase and its application in lactose hydrolysis
Abstract The present study demonstrates the immobilization of β-galactosidase from Cicer arietinum on a simple and inexpensive matrix, glutaraldehyde functionalized agarose (GFA), to suggest its potential application in hydrolyzing whey lactose in biotechnology industries. The designed matrix provided large surface area for the immobilization of β-galactos
Braz. J. Chem. Eng.. Publicado em: 2017-04
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5. Zymomonas mobilis IMMOBILIZED ON LOOFA SPONGE AND SUGARCANE BAGASSE FOR LEVAN AND ETHANOL PRODUCTION USING REPEATED BATCH FERMENTATION
Abstract Two experiments were carried out using loofa sponge and sugarcane bagasse as immobilization support. First a 2(5-2) design of experiments was realized with the following independent variables: sucrose concentration; pH; incubation time and agitation. Based on the best results observed in this experiment, the capacity for reuse of the supports in sub
Braz. J. Chem. Eng.. Publicado em: 2017-04
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6. Methacrylated Chitosan Based UV Curable Support for Enzyme Immobilization
UV curing is simple, fast and effective procedure for enzyme immobilization with minimized enzymatic activity. UV-curable methacrylated chitosan is here proposed as a support material for immobilization of lipase. The morphology of the polymeric support was characterized by scanning electron microscopy (SEM). Both covalently (CIL) and physically (PIL) immobi
Mat. Res.. Publicado em: 16/02/2017
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7. Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG
Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chlo
Braz. J. Microbiol.. Publicado em: 09/04/2013
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8. Produção de goma xantana em reator de coluna de bolha utilizando processo de batelada repetida / Xanthan gum production in a buble column reactor using a repeated bath process
The aim of this work is to evaluate xanthan production in a bubble column reactor using a repeated batch process as well as to optimize de gum production operational conditions and evaluate some of the typical steps of the recovery process. Two Xanthomonas campestris strains were evaluated: ATCC 13951 (or NRRL B-1459) and ATCC 55298. The cycles were comprise
Publicado em: 2008
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9. Estudo da produção do antibiótico antitumoral retamicina em biorreatores com células imobilizadas de Streptomyces olindensis ICB20. / Production of the antitumor antibiotic retamycin by immobilized cells of Streptomyces olindensis ICB20 in bioreactors.
The purpose of this study was to investigate the production of the antitumor antibiotic retamycin by immobilized cells of Streptomyces. olindensis ICB20 in bioreactors. Cells were immobilized by entrapment in Ca-alginate gel (3%) after being grown in Erlenmeyers (reactivation and pre-immobilization cultures). The average diameter of the Ca-alginate beads was
Publicado em: 2007
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10. Cultivo de Spirulina platensispor processo descontínuo alimentado repetitivo utilizando uréia como fonte de nitrogênio / Cultivation of Spirulina platensis by repeated fed-batch, using urea as nitrogen source
A cianobactéria Spirulina platensis possui alto teor de proteína e vem sendo cultivada fotoautotroficamente para a produção de biomassa microbiana. Embora as fontes convencionais de nitrogênio utilizadas para a produção de Spirulina spp. sejam os nitratos, há a possibilidade do emprego de uréia, utilizando o processo descontínuo alimentado, com dim
Publicado em: 2006
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11. Anaerobic ammonium oxidation in a bioreactor treating slaughterhouse wastewater
Ammonium oxidation was thought to be an exclusively aerobic process; however, as recently described in the literature, it is also possible under anaerobic conditions and this process was named ANAMMOX. This work describes the operation of a system consisting of a denitrifying reactor coupled to a nitrifying reactor used for removal of nitrogen from slaughter
Brazilian Journal of Chemical Engineering. Publicado em: 2005-12
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12. Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico
Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent
Química Nova. Publicado em: 2003-12