Respiratory Chain Inhibitors
Mostrando 1-12 de 65 artigos, teses e dissertações.
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1. Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples
OBJECTIVES: Tuberculosis is one of the most prevalent infections in humans. Although culture is the reference for diagnosis, its sensitivity is compromised, especially in paucibacillary samples. Because polymerase chain reaction (PCR) amplifies mycobacterial DNA, it is more sensitive than culture for the diagnosis of Mycobacterium tuberculosis (Mtb). Howeve
Clinics. Publicado em: 29/11/2018
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2. Mechanisms of synthesis and degradation of radical nitric oxide by mitochonderia of Arabidopsis thaliana / Mecanismos de sintese e degradação do radical oxido nitrico pela mitocondria de Arabidopsis thaliana
The objective of this work was to study the mechanisms of synthesis and degradation of NO by plant mitochondria, with emphasis on the influence of the NAD(P)H dehydrogenases and the alternative oxidase (AOX) in these processes. The experiments were carried out with mitochondria isolated from cells in culture of the model plant Arabidopsis thaliana. Initially
Publicado em: 2008
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3. Bloqueio da fosforilação oxidativa no cultivo de embriões bovinos / Oxidative phosphorylation blockage of bovine culture embryos
Although in vitro embryo production has been improved in the last 2 decades, about 60% of the oocytes do not reach the blastocyst stage and embryo quality is very variable when compared with in vivo produced embryo. This developmental block can be affected by inorganic ions, buffers, aminoacids and gaseous atmosphere composition. The knowledge that there inf
Publicado em: 2006
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4. The Respiratory System of Rhodotorula glutinis II. Mechanism of Inhibitor Tolerant Respiration
The mechanism of inhibitor-tolerant respiration in Rhodotorula glutinis was studied. This inhibitor-tolerant respiratory pathway was not due to the presence of an excess of cytochrome c oxidase, nor to the operation of an inhibitor-resistant cytochrome c oxidase. Carotenoids do not appear to be involved in this respiratory chain pathway; data are also presen
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5. Terminal branching of the respiratory electron transport chain in Neisseria meningitidis.
The respiratory components of the envelope membrane preparation of Neisseria meningitidis were investigated. Oxidase activities were demonstrated in this fraction in the presence of succinic acid, reduced nicotinamide adenine dinucleotide, and ascorbate-N,N,N',N'-tetramethyl-p-phenylene-diamine (TMPD). Differences in the kinetics of inhibition by terminal ox
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6. Differential Effect of Respiratory Inhibitors on Ergosterol Synthesis by Saccharomyces cerevisiae During Adaptation to Oxygen
The effect of different respiratory inhibitors on the ergosterol content of microaerobically grown non-proliferating yeast cultures was monitored during adaptation to oxygen. It was found that dinitrophenol, azide, and cyanide, which act on the mechanism of the respiratory chain, cause a marked stimulation of sterol production. Acriflavine and chloramphenico
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7. The Respiratory Chain of Plant Mitochondria: XVI. Interaction of Cytochrome b562 with the Respiratory Chain of Coupled and Uncoupled Mung Bean Mitochondria: Evidence for Its Exclusion from the Main Sequence of the Chain 1
Cytochromes b553, b557, and b562 of mung bean (Phaseolus aureus) mitochondria become partially reduced with endogenous substrate on addition of antimycin A to the aerobic mitochondrial suspension. Addition of ATP causes partial reoxidation of the three cytochromes. This partial oxidation by ATP is inhibited by oligomycin and reversed by uncoupler. Ubiquinone
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8. Contribution of a Cyanide-insensitive Alternate Respiratory System to Increases in Formamide Hydro-lyase Activity and to Growth in Stemphylium loti in Vitro1
Stemphylium loti, a pathogen of a cyanogenic plant, possesses a cyanide-insensitive alternate respiratory pathway. In the absence of cytochrome inhibitors, the alternate system had only a minor role in respiration. When S. loti was grown in medium amended with antimycin to block the cytochrome chain, the alternate system accounted for the total oxygen consum
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9. The Respiratory Chain of Plant Mitochondria: VII. Kinetics of Flavoprotein Oxidation in Skunk Cabbage Mitochondria
The oxidation kinetics of the two high potential flavo-proteins, one (Fphf) fluorescent and the other (Fpha) nonfluorescent, in mitochondria from skunk cabbage (Symplocarpus foetidus) spadices have been measured by combined spectrophotometry and fluorimetry. In the absence of respiratory inhibitors, both flavoproteins are oxidized at nearly the same rate wit
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10. Reduction of iron and synthesis of protoheme by Spirillum itersonii and other organisms.
Membranes from Spirillum itersonii reduce ferric iron to ferrous iron with reduced nicotinamide adenine dinucleotide or succinate as a source of reductant. Iron reduction was measured spectrophotometrically at 562 nm using ferrozine, which chelates ferrous iron specifically. Reduced nicotinamide adenine dinucleotide or succinate was also effective as a sourc
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11. The Respiratory Chain of Plant Mitochondria. II. Oxidative Phosphorylation in Skunk Cabbage Mitochondria
Mitochondria were prepared from the spadices of skunk cabbage (Symplocarpus foetidus) whose respiratory rate with succinate and malate showed 15% to 30% sensitivity to cyanide inhibition, and which showed respiratory control by added ADP. The observed respiratory control ratios ranged from 1.1 to 1.4. The change in pH of the mitochondrial suspension was reco
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12. Carbon monoxide-insensitive respiratory chain of Pseudomonas carboxydovorans.
Experiments employing electron transport inhibitors, room- and low-temperature spectroscopy, and photochemical action spectra have led to a model for the respiratory chain of Pseudomonas carboxydovorans. The chain is branched at the level of b-type cytochromes or ubiquinone. One branch (heterotrophic branch) contained cytochromes b558, c, and a1; the second