Reverse Phase High Performance Liquid Chromatography
Mostrando 1-12 de 188 artigos, teses e dissertações.
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1. Comparison of two collection methods to determine protodioscin concentrations in Brachiaria spp. and to monitor Brachiaria spp. Poisoning
RESUMO: Objetivou-se avaliar dois métodos de coleta de Brachiariaspp. (Pastejo Simulado vs Coleta Total) para determinação das concentrações de protodioscina e efetuar o monitoramento da intoxicação em rebanhos sensíveis e resistentes à intoxicação por Brachiaria spp. Foram realizadas coletas de Brachiariaspp. através de duas metodologias. A prim
Pesq. Vet. Bras.. Publicado em: 04/11/2019
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2. Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection
A novel method for the determination of aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, AFG2) in animal feed samples based on magnetic solid-phase cleanup and novel pre-column derivatization has been developed. In this work, synthesized polyvinylpyrrolidone-coated Fe3O4 magnetic nanoparticles were applied as adsorbents for rapid cleanup for extracts of anim
J. Braz. Chem. Soc.. Publicado em: 16/09/2019
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3. Automatized Separation of Fractions from Petroleum Based on Spectrophotometric Signal Derivative Using Open-Source Hardware for the Determination of Ni and V Linked to Porphyrins
An automated method based on open-source hardware was created for the separation of porphyrins fractions from crude oil, an essential step for the quantification of V and Ni as metalloporphyrins. Preparative liquid chromatography was implemented with a reverse phase gradient from MeOH to toluene. The system allows three solvents and fractions changes by usin
J. Braz. Chem. Soc.. Publicado em: 23/05/2019
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4. Screening for organic acidurias and aminoacidopathies in high-risk Brazilian patients: Eleven-year experience of a reference center
Abstract Organic acidurias and aminoacidopathies are groups of frequent inborn errors of metabolism (IEMs), which are caused by mutations in specific genes that lead to loss of protein/enzyme or transport function with important deleterious effects to cell metabolism. Since a considerable number of such disorders are potentially treatable when diagnosed at a
Genet. Mol. Biol.. Publicado em: 11/04/2019
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5. Ajmalicine Bioproduction in Catharanthus Roseus (L) G. Don Inoculated with Arbuscular Mycorrhiza and Fertilized with Nitrogen
ABSTRACT Catharanthus roseus (L) G. Don (Madagascar periwinkle) belongs to the Apocynaceace family and is widely spread throughout tropical and subtropical regions of the world. The plant produces several important alkaloids, such as ajmalicine and serpentine, which are used in the treatment of circulatory diseases. The potential of inoculation with arbuscul
Rev. Bras. Ciênc. Solo. Publicado em: 23/07/2018
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6. Development and validation of stability indicating liquid chromatographic (RP-HPLC) method for estimation of ubidecarenone in bulk drug and formulations using quality by design (QBD) approach
ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) desi
Braz. J. Pharm. Sci.. Publicado em: 05/03/2018
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7. FOURIER TRANSFORM INFRARED SPECTROSCOPY, THERMOGRAVIMETRIC ANALYSIS, SCANNING ELECTRON MICROSCOPY AS SUPPORTING TOOLS IN QUALITY CONTROL OF ANTIPARASITICS
This study shows that Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) can be used as supporting tools for the evaluation of the quality of antiparasitics. In addition, an analytical methodology was developed and validated to quantify simultaneously thiabendazole (TB), febantel (FB), tol
Quím. Nova. Publicado em: 2018-03
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8. Development and validation of an analytical method using High Performance Liquid Chromatography (HPLC) to determine ethyl butylacetylaminopropionate in topical repellent formulations
ABSTRACT Diseases caused by insects are frequent in poor countries, leading to epidemic scenarios in urban areas; e.g., Dengue, Zika and Chikungunya. For this reason, the development of a safe and efficient topical formulation is essential. Ethyl butylacetylaminopropionate (EB) is a mosquito repellent developed by Merck, which is used in products for adults,
Braz. J. Pharm. Sci.. Publicado em: 22/06/2017
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9. A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles
ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 co
Braz. J. Pharm. Sci.. Publicado em: 22/06/2017
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10. Production of hydrolysate from processed Nile tilapia (Oreochromis niloticus) residues and assessment of its antioxidant activity
Abstract The objective of this work was to produce protein hydrolysates from by-products of the Nile tilapia fileting process, and to assess the effects of different hydrolysis times on the antioxidant activity of the hydrolysed animal-based protein, in free form and incorporated into a food matrix. Gutted tilapia heads and carcasses were hydrolysed by Alcal
Food Sci. Technol. Publicado em: 29/08/2016
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11. Development of HPLC Analytical Techniques for Diterpene Glycosides from Stevia rebaudiana (Bertoni) Bertoni: Strategies to Scale-Up
Stevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Different stationary phases/columns were used: rever
J. Braz. Chem. Soc.. Publicado em: 2016-08
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12. Determination of Phenolic Acids and Quercetin in Brazilian Red Wines from Vale do São Francisco Region Using Liquid-Liquid Ultrasound-Assisted Extraction and HPLC-DAD-MS
This work describes a method for the determination of gallic, caffeic and p-coumaric acids, as well as quercetin, in Brazilian red wines by employing reverse phase high performance liquid chromatography (RP HPLC) coupled to diode array and mass detectors. The method was performed using a sample volume of 10 µL. The limits of detections were 0.36, 0.27, 0.33
J. Braz. Chem. Soc.. Publicado em: 2016-06