Reversed Phase Chromatography
Mostrando 1-12 de 382 artigos, teses e dissertações.
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1. Per-Aqueous Liquid Chromatography for the Separation of Polar Compounds Preparation and Characterization of Poly(ethylene oxide co dimethylsiloxane) Based Stationary Phase
Poly(ethylene oxide-co-dimethylsiloxane) was thermally immobilized on silica particles-Si(PEO)-to separate small polar compounds with water-rich mobile phases. Poly(ethylene oxide-co-dimethylsiloxane) content on Si(PEO) stationary phase was optimized using a central composite design. Infrared spectroscopy, scanning electron microscopy, and thermogravimetric
Journal of the Brazilian Chemical Society. Publicado em: 2022
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2. Development and application of a test mixture for untargeted liquid chromatography-mass spectrometry analysis of urine samples
Metabolic profiling of complex biological matrices based on liquid chromatography-mass spectrometry (LC-MS) allows detecting a wide range of metabolites with distinct concentrations and physicochemical properties. Given the complexity of samples and the necessity of a comprehensive approach in untargeted metabolomics, quality control strategies are mandatory
Química Nova. Publicado em: 2022
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3. Development and Validation of a Rapid and Reliable HPLC-FLD Method for the Quantification of Ciprofloxacin and Enrofloxacin Residues in Zea mays
The accumulation of antibiotics by plants is a currently concern associated to yield reduction and transference of antibiotic residues along the food web. Maize is a staple food in many parts of the world; it is also considered an important ingredient in animal nutrition. Considering the lack of validated analytical methods for the analysis of ciprofloxacin
Journal of the Brazilian Chemical Society. Publicado em: 2022
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4. One-Step Isolation of Monoterpene Indole Alkaloids from Psychotria leiocarpa Leaves and Their Antiviral Activity on Dengue Virus Type-2
The leaf MeOH extract of Psychotria leiocarpa (Rubiaceae) showed in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activity in human hepatocarcinoma cell lineage (HepG2). A one-step and cost-effective reversed-phase solid-phase extraction method based on high-performance liquid chromatography (HPLC) parameters allowed the isolation, directly fr
J. Braz. Chem. Soc.. Publicado em: 2020-10
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5. Determinação de docetaxel e metabólitos em plasma por UPLC-MS/MS: desenvolvimento de metodologia analítica e aplicação clínica
A highly sensitive ultra-performance liquid chromatography tandem mass spectrometry method for the determination of Docetaxel and metabolites (M1+M3) in human plasma samples was developed and validated. The method employs a liquid-liquid extraction and a reversed phase separation Acquity® C18 column BEH C18 (150 x 2.1 mm, 1.7 µm). Mobile phase was a mixtur
Quím. Nova. Publicado em: 2020-04
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6. Application of a New Validated HPLC-PDA Method for Simultaneous Determination of Curcumin and Melatonin in Hyaluronic Acid-Coated Nanoemulsions
In this study, a high-performance liquid chromatography with photodiode array detector (HPLC-PDA) method was developed and validated to simultaneously determine curcumin (CUR) and melatonin (MEL) in hyaluronic acid-coated nanoemulsions, a novel targetable delivery system to CD44 receptors overexpressed in many types of tumors. Chromatographic analyses were p
J. Braz. Chem. Soc.. Publicado em: 2020-03
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7. Evaluation of recombinant human interferon beta 1b by liquid chromatography methods and bioassay
Recombinant human interferon beta 1b (rhIFNβ-1b) is clinically used to treat multiple sclerosis. A reversed-phase liquid chromatography (RP-LC) method was carried out on a Jupiter C4 column (250 mm × 4.6 mm i.d.). The mobile phase A consisted of 0.1% trifluoroacetic acid (TFA) in water, and the mobile phase B was acetonitrile with 0.1% TFA run at a flow ra
Braz. J. Pharm. Sci.. Publicado em: 24/10/2019
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8. A New Tetraglycosylated Flavonoid from Leaves of Platycyamus regnellii Benth. Isolated by High-Speed Countercurrent Chromatography
A new tetraglycosylated flavonoid derivative of kaempferol was isolated from the butanol extract of leaves of Platycyamus regnellii (Fabaceae) using high-speed countercurrent chromatography (HSCCC). The butanol extract (960.5 mg) was initially fractionated with the solvent system EtOAC-BuOH-H2O 2:8:10 (v/v/v) (elution in normal phase mode). The fractions con
J. Braz. Chem. Soc.. Publicado em: 24/10/2019
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9. LC-UV method to assay raloxifene hydrochloride in rat plasma and its application to a pharmacokinetic study
A specific, precise, and accurate LC-UV method was developed and validated to assay raloxifene hydrochloride in rat plasma. Raloxifene was analyzed after liquid-liquid extraction and quantified by reversed phase liquid chromatography (C18 column) using acetonitrile and ammonium acetate buffer 0.05 M (pH 4.0) as mobile phase at a flow rate of 1 mL.min-1 and U
Braz. J. Pharm. Sci.. Publicado em: 30/09/2019
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10. Subproteome of Lachesis muta rhombeata venom and preliminary studies on LmrSP-4, a novel snake venom serine proteinase
Abstract Background: Lachesis muta rhombeata is one of the venomous snakes of medical importance in Brazil whose envenoming is characterized by local and systemic effects which may produce even shock and death. Its venom is mainly comprised of serine and metalloproteinases, phospholipases A2 and bradykinin-potentiating peptides. Based on a previously report
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 15/04/2019
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11. Antivenomic approach of different Crotalus durissus collilineatus venoms
Abstract Background: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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12. Study on the Mixed-Mode HPLC Separation of Shuang-Huang-Lian and Xue-Bi-Jing Injections Based on an Ionic Liquid Column
Both Shuang-Huang-Lian and Xue-Bi-Jing injections are clinically proven efficient and low cost medicines. However, the studies of their chromatographic separation are limited. In this study, a column based on ionic liquid stationary phase was applied for the first time for the analysis of these two injections. For Shuang-Huang-Lian injection, U-shaped plots
J. Braz. Chem. Soc.. Publicado em: 2018-07