Rna Helicases
Mostrando 1-12 de 156 artigos, teses e dissertações.
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1. Papel do PTPN2 e MDA5, dois genes candidatos para diabete melito tipo 1, nas respostas das células beta pancreáticas a citocinas pró-‐inflamatórias e ao RNA de fita dupla intracelular
Na patogênese do diabete melito tipo 1 (DM1) vários genes e fatores ambientais, como as infecções virais, interagem para iniciar um ataque autoimune contra as células beta pancreáticas. Durante a fase inicial desse processo, as células beta desempenham um papel importante através da promoção de um “diálogo” com o sistema imune. Recentemente, o
Publicado em: 2010
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2. Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigrom
Zoologia (Curitiba). Publicado em: 2009-06
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3. Evolução do reparo por excisão de nucleotídeo
The phylogeny is one of the main activities of the modern taxonomists and a way to reconstruct the history of the life through comparative analysis of these sequences stored in their genomes aimed find any justification for the origin or evolution of them. Among the sequences with a high level of conservation are the genes of repair because it is important f
Publicado em: 2008
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4. Study of the function of the protein Nop53p in Saccharomyces cerevisiae / Caracterização da função da proteína Nop53p de Saccharomyces cerevisiae
In eukaryotes, the rRNA processing depends on several factors, such as, endonucleases, exonucleases, RNA helicases, rRNA modifying enzymes and components of the snoRNPs. With the purpose of characterizing new proteins involved in pre-rRNA processing, Nop53p was identified interacting with the nucleolar protein Nop17p in a two hybrid assay. The conditional ye
Publicado em: 2007
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5. Análise estrutural e funcional de cofatores do exossomo em Saccharomyces cerevisiae e Pyrococcus / Structural and functional analysis of exosome cofactors in Saccharomyces cerevisiae and Pyrococcus
A síntese ribossomal é uma das maiores atividades em células eucarióticas. Este processo inicia-se no nucléolo e é finalizado após a exportação das subunidades 40S e 60S para o citoplasma. Três dos RNAs ribossomais de eucariotos (18S, 5.8S e 25S) são sintetizados como um transcrito primário de 35S, o qual é processado através de uma complexa e
Publicado em: 2006
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6. Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12
DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evalu
Brazilian Journal of Medical and Biological Research. Publicado em: 2001-10
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7. Detection of dsRNA-binding domains in RNA helicase A and Drosophila maleless: implications for monomeric RNA helicases.
Searches with dsRNA-binding domain profiles detected two copies of the domain in each of RNA helicase A, Drosophila maleless and C. elegans ORF T20G5-11 (of unknown function). RNA helicase A is unusual in being one of the few characterised DEAD/DExH helicases that are active as monomers. Other monomeric DEAD/DExH RNA helicases (p68, NPH-II) have domains that
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8. Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases.
Comparative studies of sequence motifs in the RNA polymerases and nucleic acid helicases of positive-sense RNA plant viruses have provided a new scheme for the classification of these pathogens. We propose a new luteovirus supergroup which should be added to the already described Sindbisvirus-like and picornavirus-like supergroups. Sequence motifs of nucleic
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9. Visualization of unwinding activity of duplex RNA by DbpA, a DEAD box helicase, at single-molecule resolution by atomic force microscopy
The Escherichia coli protein DbpA is unique in its subclass of DEAD box RNA helicases, because it possesses ATPase-specific activity toward the peptidyl transferase center in 23S rRNA. Although its remarkable ATPase activity had been well defined toward various substrates, its RNA helicase activity remained to be characterized. Herein, we show by using
The National Academy of Sciences.
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10. Choosing between DNA and RNA: the polymer specificity of RNA helicase NPH-II
NPH-II is a prototypical member of the DExH/D subgroup of superfamily II helicases. It exhibits robust RNA helicase activity, and a detailed kinetic framework for unwinding has been established. However, like most SF2 helicases, there is little known about its mode of substrate recognition and its ability to differentiate between RNA and DNA substrates. Here
Oxford University Press.
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11. Rearrangement of structured RNA via branch migration structures catalysed by the highly related DEAD-box proteins p68 and p72
RNA helicases, like their DNA-specific counterparts, can function as processive enzymes, unwinding RNA with a defined step size in a unidirectional fashion. Recombinant nuclear DEAD-box protein p68 and its close relative p72 are reported here to function in a similar fashion, though the processivity of both RNA helicases appears to be limited to only a
Oxford University Press.
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12. A Cold Shock-Induced Cyanobacterial RNA Helicase
The ability to modify RNA secondary structure is crucial for numerous cellular processes. We have characterized two RNA helicase genes, crhB and crhC, which are differentially expressed in the cyanobacterium Anabaena sp. strain PCC 7120. crhC transcription is limited specifically to cold shock conditions while crhB is expressed under a variety of conditions,
American Society for Microbiology.