Rnai Feeding
Mostrando 1-7 de 7 artigos, teses e dissertações.
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1. Acúmulo de ricina em sementes de mamona e silenciamento do gene em plantas geneticamente modificadas
Castor bean (Ricinus communis L.) is an oilseed of great economic and social importance. The oil extracted from its seeds is used in biodiesel production and as raw material for chemicals, cosmetics and lubricants. Besides the oil extracted from the seeds, their production chain generates side-products, which can be used as organic fertilizer of good quality
Publicado em: 2010
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2. Aspectos moleculares da imunidade de Lutzomyia longipalpis (Díptera:Psychodidae). / Molecular aspects of immunity of Lutzomyia longipalpis (Díptera: Psychodidae).
Sandflies are known for the transmission of leishmaniasis but can also transmit viral and bacterial diseases. Also, sandflies get in contact with several insect pathogens like fungi and helminths. The strategies used by sandflies for defense against pathogens are mostly unknown. The main infection path is through blood feeding and the midgut is the first imm
Publicado em: 2007
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3. RNAs de fita dupla oferecidos na dieta de larvas causam alterações fisiológicas no desenvolvimento das castas de Apis mellifera / Double-stranded RNA ingested by Apis mellifera larvae promotes phisiological disturbs in caste development
Abelhas adultas produzem vitelogenina, a principal proteína da hemolinfa. Ela está envolvida na reprodução, comportamento, imunidade, longevidade e regulação da organização social. A interferência por RNA interference é a mais promissora ferramenta para estudos de função gênica, baseada na introdução de duplex de RNA (dsRNA) que induz a degrad
Publicado em: 2007
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4. Genome-Wide RNAi of C. elegans Using the Hypersensitive rrf-3 Strain Reveals Novel Gene Functions
RNA-mediated interference (RNAi) is a method to inhibit gene function by introduction of double-stranded RNA (dsRNA). Recently, an RNAi library was constructed that consists of bacterial clones expressing dsRNA, corresponding to nearly 90% of the 19,427 predicted genes of C. elegans. Feeding of this RNAi library to the standard wild-type laboratory strain Br
Public Library of Science.
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5. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans
RNAi can be achieved by feeding worms Escherichia coli expressing dousble-stranded RNA corresponding to a specific gene. An optimized feeding method is presented that results in phenotypes at least as strong as those produced by direct injection of RNA for embryonic lethal genes, and stronger for genes with post-embryonic phenotypes.
BioMed Central.
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6. Transgenic alteration of Toll immune pathway in the female mosquito Aedes aegypti
Reverse genetics is a powerful tool for understanding gene functions and their interactions in the mosquito innate immunity. We took the transgenic approach, in combination with the RNA interference (RNAi) technique, to elucidate the role of mosquito REL1, a homolog of Drosophila Dorsal, in regulation of Toll immune pathway in the mosquito Aedes aegypti. By
National Academy of Sciences.
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7. RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny in the mosquito Aedes aegypti
Blood feeding tightly regulates the reproductive cycle in anautogenous mosquitoes. Vitellogenesis (the synthesis of yolk protein precursors) is a key event in the mosquito reproductive cycle and is activated in response to a blood meal. Before blood feeding, Aedes aegypti is in a state of reproductive arrest during which the yolk protein precursor genes (YPP
National Academy of Sciences.