Rrna Processing
Mostrando 1-12 de 400 artigos, teses e dissertações.
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1. Survey of molds, yeast and Alicyclobacillus spp. from a concentrated apple juice productive process
Bacteria and molds may spoil and/or contaminate apple juice either by direct microbial action or indirectly by the uptake of metabolites as off-flavours and toxins. Some of these microorganisms and/or metabolites may remain in the food even after extensive procedures. This study aim to identify the presence of molds (including heat resistant species) and Ali
Braz. J. Microbiol.. Publicado em: 2014
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2. Caracterização funcional das proteínas NIP7 e FTSJ3 no processamento do RNA ribossomal em células humanas / Functional characterization of proteins NIP7 and FTSJ3 in ribosomal RNA processing in human cells
Estudos prévios realizados em nosso laboratório demonstraram a interação entre as proteínas humanas SBDS e NIP7. SBDS participa da biogênese de ribossomos e sua deficiência está associada à síndrome de Shwachman- Bodian-Diamond. NIP7 é uma proteína conservada e já foi caracterizada em levedura, onde participa da formação da subunidade ribossom
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/06/2012
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3. Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salm
Rev. Bras. Cienc. Avic.. Publicado em: 2012-09
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4. Footprints of a trypanosomatid RNA world: pre-small subunit rRNA processing by spliced leader addition trans-splicing
The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family. This process is an inter-molecular splicing reaction that follows the same basic rules of cis-splicing reactions. In this study, we demons
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-06
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5. Caracterização funcional da proteína Nop8p de Saccharomyces cerevisiae / Functional characterization of the Saccharomyces cerevisiae nucleolar protein Nop8p
A proteína nucleolar Nop8p de levedura foi identificada inicialmente através de sua interação com Nip7p e está envolvida na formação da subunidade ribossomal 60S. A depleção de Nop8p em células de levedura leva à degradação prematura dos rRNAs, porém o mecanismo bioquímico responsável por este fenótipo ainda não é conhecido. Neste trabalho
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/10/2011
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6. Distribution of prokaryotic organisms in a tropical estuary influenced by sugar cane agriculture in northeast Brazil
In a joint Brazilian-German case study, distribution patterns of microorganisms were compared with environmental variables in the tropical coastal Manguaba lagoon in northeast Brazil, which is situated downstream of several sugar cane processing plants . 16S rDNA and 16S rRNA single strand conformation polymorphism (SSCP) gene fingerprinting were used to fol
Brazilian Journal of Microbiology. Publicado em: 2010-12
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7. Study of the function of the protein Nop53p in Saccharomyces cerevisiae / Caracterização da função da proteína Nop53p de Saccharomyces cerevisiae
In eukaryotes, the rRNA processing depends on several factors, such as, endonucleases, exonucleases, RNA helicases, rRNA modifying enzymes and components of the snoRNPs. With the purpose of characterizing new proteins involved in pre-rRNA processing, Nop53p was identified interacting with the nucleolar protein Nop17p in a two hybrid assay. The conditional ye
Publicado em: 2007
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8. Cloning of a gene involved in rRNA precursor processing and 23S rRNA cleavage in Rhodobacter capsulatus.
In Rhodobacter capsulatus wild-type strains, the 23S rRNA is cleaved into [16S] and [14S] rRNA molecules. Our data show that a region predicted to form a hairpin-loop structure is removed from the 23S rRNA during this processing step. We have analyzed the processing of rRNA in the wild type and in the mutant strain Fm65, which does not cleave the 23S rRNA. I
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9. The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking.
The first processing event that mouse pre-rRNA undergoes occurs within the external transcribed spacer and is efficiently reproduced in vitro. Analysis with nondenaturing polyacrylamide gels revealed the formation of heparin-resistant complexes of retarded electrophoretic mobility on the substrate rRNA. The specificity of these complexes was demonstrated by
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10. In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
DNA oligonucleotide complementary to sequences in the 5' third of U3 snRNA were injected into Xenopus oocyte nuclei to disrupt endogenous U3 snRNA. The effect of this treatment on rRNA processing was examined. We found that some toads have a single rRNA processing pathway, whereas in other toads, two rRNA processing pathways can coexist in a single oocyte. U
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11. Nucleotide sequence determining the first cleavage site in the processing of mouse precursor rRNA.
The first step in the processing of 47S precursor rRNA in mouse cells is reproduced in vitro in an S-100 transcription reaction and consists of an endonucleolytic cleavage at residue +650 of the primary transcript followed by rapid degradation of the fragment upstream from residue +650. An analogous processing occurs in human rRNA. The mouse and human rRNA s
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12. Primary processing of mammalian rRNA involves two adjacent cleavages and is not species specific.
The primary transcript of the mouse rRNA gene is rapidly processed at nucleotide approximately +650 both in vivo and in vitro. Using run-off transcription in a mouse cell extract as well as S1 nuclease and primer extension analysis of cellular RNA, we demonstrated that this primary processing actually results in the formation of two species of downstream RNA