Rt Pcr Multiplex
Mostrando 1-12 de 75 artigos, teses e dissertações.
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1. Influência do uso precoce de antimicrobiano na sanidade de bezerras holandesas no primeiro mês de vida
RESUMO: O uso precoce de antimicrobianos tem sido adotado em muitas fazendas para profilaxia das diarreias e doença respiratória em bezerras, no entanto existem controvérsias sobre os beneficios desta prática na saúde desses animais. Esta pesquisa avaliou a influência do uso precoce de antimicrobiano na sanidade e desempenho de bezerras holandesas rec�
Pesq. Vet. Bras.. Publicado em: 2020-01
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2. Campylobacter jejuni and Campylobacter coli originated from chicken carcasses modulate their transcriptome to translate virulence genes in human cells
RESUMO: O objetivo foi determinar a disseminação de perfis geneticamente semelhantes de Campylobacter em carcaças de frango e avaliar sua capacidade de produzir transcritos para os genes ciaB, dnaJ, p19 e sodB, antes e após o cultivo em células Caco-2. As cepas utilizadas foram isoladas de 420 amostras de carcaças de frango resfriadas e congeladas pron
Pesq. Vet. Bras.. Publicado em: 14/10/2019
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3. Human virome in nasopharynx and tracheal secretion samples
BACKGROUND In Brazil the implementation of the Sentinel Surveillance System of Influenza began in 2000. Central public health laboratories use reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for diagnosis of respiratory viruses, but this protocol identifies only specific targets, resulted in inconclusive diagnosis for many samples. T
Mem. Inst. Oswaldo Cruz. Publicado em: 03/10/2019
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4. Imported case of Dengue virus 3 genotype I in Rio de Janeiro state, Brazil
The dengue virus (DENV), of the genus Flavivirus (Flaviviridae), has four antigenically distinct serotypes, of which DENV-3 is classified into five genotypes. Here, we describe the detection of DENV-3 genotype I in sera of a Brazilian patient travelling from Singapore to Rio de Janeiro, Brazil, by using multiplex real-time RT-PCR, DNA sequencing of the whole
Mem. Inst. Oswaldo Cruz. Publicado em: 25/06/2018
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5. Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods that facilitate a rapid and accurate diagnosis is cru
Mem. Inst. Oswaldo Cruz. Publicado em: 26/02/2018
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6. Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
ABSTRACT We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated f
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-07
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7. Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
BACKGROUND Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is
Mem. Inst. Oswaldo Cruz. Publicado em: 06/04/2017
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8. FIRST MOLECULAR DETECTION AND VP7 (G) GENOTYPING OF GROUP A ROTAVIRUS BY SEMI-NESTED RT-PCR FROM SEWAGE IN NIGERIA
SUMMARY Rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. Our aim was to detect and genotype rotaviruses from sewages in Nigeria. One hundred and ninety sewage samples were collected between June 2014 and January 2015. The two phase concentration method using PEG 600
Rev. Inst. Med. trop. S. Paulo. Publicado em: 03/11/2016
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9. Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil
Resumo: O objetivo deste trabalho foi descrever a ocorrência do Tulip breaking virus (TBV, sinonímia Lily mottle virus - LMoV) quarentenário e do Lily symptomless virus (LSV) e suas análises moleculares, para fornecer dados para a remoção do TBV da lista brasileira de pragas quarentenárias A1, pois este vírus está disseminado pelos principais cultiv
Pesq. agropec. bras.. Publicado em: 2016-05
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10. Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiabá, state of Mato Grosso, Brazil
This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female pools (n =
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-01
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11. Surto de dengue em Mato Grosso, centro-oeste do Brasil
O vírus da dengue (DENV) é o arbovirus mais frequente no mundo. Neste estudo, é relatada uma epidemia de grandes proporções no estado de Mato Grosso (MT). Amostras de soro de 604 pacientes com doença febril aguda a menos de 5 dias foram inoculadas em células C6/36 seguida de Imunofluorescência indireta para os sorotipos do DENV e vírus da febre amar
Rev. Inst. Med. trop. S. Paulo. Publicado em: 2015-12
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12. Vírus da encefalite de Saint Louis em Mato Grosso, Centro-Oeste, Brasil
O vírus da dengue (DENV), frequentemente envolvido em epidemias de grande proporção, e o vírus da febre amarela (YFV), responsável por surtos silvestres esporádicos, são considerados os flavivírus circulantes mais importantes no Brasil. Por este motivo, o diagnóstico laboratorial de doença febril aguda indiferenciada durante períodos epidêmicos �
Rev. Inst. Med. trop. S. Paulo. Publicado em: 2015-06