S6k Recombinant Protein
Mostrando 1-12 de 136 artigos, teses e dissertações.
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1. Molecular Characterization of a MYB Protein from Oryza sativa for its Role in Abiotic Stress Tolerance
ABSTRACT The MYB family represents one of the most abundant classes of transcriptional regulators that perform pivotal role under different developmental processes and abiotic stresses. In present study, a MYB gene from Oryza sativa was selected for functional characterization. Bioinformatics analysis revealed that OsMYB1 cDNA encodes R2-R3 type DNA binding
Braz. arch. biol. technol.. Publicado em: 22/06/2017
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2. ExpressÃo de uma quitinase de Chromobacterium Violaceum em Pichia Pastoris: purificaÃÃo e caracterizaÃÃo parcial da proteÃna recombinante / Expression of a chitinase from Chromobacterium Violaceum in Pichia pastoris: purification and partial characterization of recombinant protein
A utilizaÃÃo de microrganismos como sistemas heterÃlogos de expressÃo de proteÃnas tem se mostrado uma estratÃgia alternativa e/ou complementar aos passos tradicionais utilizados no processo de purificaÃÃo de proteÃnas. Diante deste contexto, quitinases (EC 3.2.1.14), enzimas hidrolÃticas capazes de degradar quitina, tÃm sido expressas em diferent
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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3. Obtenção de proteína recombinante baseada em antígenos do líquido vesicular de Taenia crassiceps: aplicação no imunodiagnóstico da neurocisticercose / Recombinant protein obtainment based on antigens from Taenia crassiceps vesicular fluid: application on immunodiagnostics of neurocisticercosis
A neurocisticercose (NC) é uma doença provocada por larvas de Taenia solium (Tso) no sistema nervoso central. Seu diagnóstico fundamenta-se em critérios clínicos, epidemiológicos e laboratoriais. A utilização de antígenos parasitários no imunodiagnóstico apresenta desvantagens como: necessidade de animais, ausência de homogeneidade entre lotes, b
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/02/2011
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4. Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K / Standardization of the heterologous expression and of a model assay of activity for the human protein kinase S6K
The 70kDa ribosomal S6 protein kinase 1 (S6K1) is a phosphoprotein involved in the regulation of genes related to translational control in mammals. S6K1 shows distinct nuclear (a1) and cytoplasmic (a2) forms. Phosphorylation of the S6K1 best characterized target, the protein of the small ribosomal subunit (RPS6), has been generally associated to the selectiv
Publicado em: 2009
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5. Biochemical and immunological characterization of ATP- diphosphohydrolases 1 and 2 from Schistosoma mansoni parasite / Caracterização bioquímica e imunológica das enzimas recombinantes ATP-difosfohidrolases 1 e 2 do parasita Schistosoma mansoni
ATPDases or ATP-diphosphohydrolases are enzymes that cleave ATP and ADP to AMP and Pi and are involved in inhibition of platelet aggregation. In the parasite Schistosoma mansoni our group had identified and cloned the ATPDase1 gene and localized the protein on the tegument surface. Recently, we cloned the ATPDase2 gene using S. mansoni EST databank informati
Publicado em: 2008
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6. Expressão heteróloga do gene Hxyn2 do fungo Humicola grisea var. thermoidea em Pichia pastoris: Produção e purificação da enzima HXYN2r e aplicação em testes de panificação / Heterologous expression of the gene Hxyn2 fungus Humicola grisea var. thermoidea in Pichia pastoris: production and purification of the enzyme HXYN2r testing and application in bakery
Endoxylanases are the main group of enzymes involved in the hydrolysis of xylan. This enzymes have application in industrial proposes, like as drink, food, feed, clothes industries and for bleaching cellulose paper pulps. In bread making the xylanases have been used to improve processing and product quality of loaf, leading soft dough and loaf with larger vo
Publicado em: 2008
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7. Expressão de uricase de Bacillus subtilis em escherichia coli
Bacillus subtilis URICASE EXPRESSION IN Escherichia coli Uricase, an enzyme that converts uric acid into allantoin, is commonly used in commercial kits for uric acid detection. Aiming to produce this enzyme in industrial scale using Genetic Engineering techniques, the uricase gene of Bacillus subtilis subtilis was cloned and expressed in Escherichia coli. In
Publicado em: 2007
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8. Obtaining of recombinant strains of the yeast Kluyveromyces lactis producers of streptavidin / Obtenção de linhagens de Kluyveromyces lactis recombinantes produtoras de estreptavidina
The high affinity interaction streptavidin-biotin provides an excellent system for industrial enzyme separation or immobilization involving a fused streptavidin-enzyme and a biotinylated support. In order to produce proteins with affinity to biotin, we have modified the commercial K. lactis system, pKLAC1 (New England Biolabs), a LAC4 promoter-driven integra
Publicado em: 2007
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9. Analise funcional e estrutural da proteina HBx do virus da Hepatite B / Functional and structural analysis of the hepatitis B virus X protein
Chronic infection of the hepatitis B virus (HBV) is one of the causes leading to liver cancer. The HBV genome encodes the 17 kDa onco-protein HBx, a multi-functional protein that contains 10 cysteine residues and is related to induce liver cancer in transgenic mice. The exact function of HBx is still unknown. However, it has been shown that HBx is a potent t
Publicado em: 2005
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10. r-Sm14 - pRSETA efficacy in experimental animals
Previous studies carried out with Sm14 in experimental vaccination against Schistosoma mansoni or Fasciola hepatica infections were performed with recombinant Sm14 (rSm14) produced in Escherichia coli by the pGEMEX system (Promega). The rSm14 was expressed as a 40 kDa fusion protein with the major bacteriophage T7 capsid protein. Vaccination experiments with
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-09
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11. Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor
Abnormal production of interferon alpha (IFN-a) has been found in certain autoimmune diseases and can be also observed after prolonged therapy with IFN-a. IFN-a can contribute to the pathogenesis of allograft rejection in bone marrow transplants. Therefore, the development of IFN-a inhibitors as a soluble receptor protein may be valuable for the therapeutic
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-07
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12. Signal transduction induced in Trypanosoma cruzi metacyclic trypomastigotes during the invasion of mammalian cells
Penetration of Trypanosoma cruzi into mammalian cells depends on the activation of the parasite's protein tyrosine kinase and on the increase in cytosolic Ca2+ concentration. We used metacyclic trypomastigotes, the T. cruzi developmental forms that initiate infection in mammalian hosts, to investigate the association of these two events and to identify the v
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-03