Sandwich Enzyme Linked Immunosorbent Assay
Mostrando 1-12 de 175 artigos, teses e dissertações.
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1. Development of a sandwich enzyme-linked immunosorbent assay based on single-domain antibody for detecting goose parvovirus infection
RESUMO A infecção por parvovírus de ganso (GPV) é uma doença infecciosa altamente patogênica em gansinhos e patinhos de Muscovy. Para detectar o antígeno GPV, desenvolvemos um novo ensaio imunoenzimático (ELISA) sanduíche usando um anticorpo de domínio único de cadeia pesada específico contra a proteína GPV NS1 como anticorpo de detecção. Os l
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2022
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2. Enhanced serum interferon-lambda 1/interleukin-29 levels in patients with psoriasis vulgaris
Abstract Background: Interferon (IFN)-λ1, also named Interleukin (IL)-29, is a new member of the Type III IFN or IFN-λ family. IL-29 plays an important role in the pathogenesis of many types of autoimmune and inflammatory diseases. Objective: To study the role of IL-29 in the pathogenesis of psoriasis vulgaris. Methods: The authors detected the serum le
An. Bras. Dermatol.. Publicado em: 2021-08
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3. Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements
Abstract Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodo
J. Appl. Oral Sci.. Publicado em: 10/01/2020
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4. Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases
Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal an
Braz J Med Biol Res. Publicado em: 30/09/2019
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5. Relationships of interleukin-10 with the regulatory T cell ratio and prognosis of cervical cancer patients
OBJECTIVE: This study investigated serum interleukin-10 (IL-10) levels, changes in peripheral blood CD4+CD25+ regulatory T cell (PBCDT) ratios, and the prognosis of cervical cancer (CC) patients. METHODS: Seventy patients with CC composed the observation group, and 70 healthy subjects composed the control group. The PBCDT ratios in the CC patients and heal
Clinics. Publicado em: 03/12/2018
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6. Sandwich ELISA com duplo anticorpo baseado no circovirus suíno tipo 2 (PCV2) produzido em cultivo celular para detecção de anticorpos
RESUMO: Há poucos relatos na literatura de métodos de ELISA (Enzyme-linked immunosorbent assay), para a detecção de anticorpos contra o circovírus suíno tipo 2 (PCV2), baseados em antígenos produzidos em cultivo celular, bem como uma escassez de trabalhos descrevendo técnicas de purificação viral para os membros da família Circoviridae. Isso ocorr
Pesq. Vet. Bras.. Publicado em: 2016-12
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7. Serum big endothelin-1 as a biomarker in oral squamous cell carcinoma patients: an analytical study
Detection of abnormally elevated levels of molecules in patients with oral cancer may be useful in early diagnosis. These markers can be included in current Histopathology grading and in TNM staging systems of Oral Squamous Cell Carcinoma (OSCC) to make it more efficient. Several pro-angiogenic molecules have been assessed for the same reason. Endothelin-1 (
J. Appl. Oral Sci.. Publicado em: 2015-10
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8. Main viruses in sweet cherry plantations of Central-Western Spain
Sweet cherry trees (Prunus avium L.) are susceptible to a range of diseases, but there have been no studies to date about the viral infection of sweet cherry trees in Spain. To determine the phytosanitary status of Spanish sweet cherry plantations, the incidence and leaf symptoms induced by Prune dwarf (PDV), Prunus necrotic ringspot (PNRSV) and Apple chloro
Sci. agric. (Piracicaba, Braz.). Publicado em: 2015-02
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9. Schistosoma mansoni antigens alter the cytokine response in vitro during cutaneous leishmaniasis
Schistosoma mansoni infection or associated products are able to down-modulate the type 1 CD4+ T cell inflammatory response characteristic of autoimmune diseases. In this study, we evaluated how S. mansoni antigens altered the immune response that was induced by the soluble Leishmania antigen (SLA) from cutaneous leishmaniasis (CL) patients. Cytokines were m
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-11
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10. Caracterização antigênica e molecular de isolados e desenvolvimento de testes sorológicos para detecção de anticorpos contra o vírus da raiva
Esta tese compreende estudos sobre diagnóstico, caracterização antigênica e molecular de amostras do vírus da raiva e sobre o desenvolvimento de testes sorológicos para detecção de anticorpos contra o vírus rábico. O primeiro capítulo descreve dois casos de raiva no Estado do Rio Grande do Sul (RS). O primeiro trabalho do primeiro capítulo descre
Publicado em: 2011
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11. Ocorrência de vírus em batata em sete estados do Brasil
As viroses causam rápida degenerescência dos tubérculos-sementes de batata. Em condições tropicais, em que a presença de afídeos vetores é constante e a estrutura das populações de vírus é dinâmica, a pressão das doenças é enorme. Conhecer essa dinâmica é uma ferramenta importante para a sustentabilidade da produção de batata. Realizou-se
Horticultura Brasileira. Publicado em: 2009-12
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12. Biotin-avidin sandwich elisa with specific human isotypes IgG1 and IgG4 for Culicidae mosquito blood meal identification from an epizootic yellow fever area in Brazil
With a view toward investigating the feeding behavior of Culicidae mosquitoes from an area of epizootic yellow fever transmission in the municipalities of Garruchos and Santo Antônio das Missões, Rio Grande do Sul State, Brazil, specimens were collected by aspiration from September 2005 to April 2007. The engorged females were submitted to blood meal ident
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009