Schiff Reagent
Mostrando 1-12 de 47 artigos, teses e dissertações.
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1. PAPEL INDICADOR COLORIMÉTRICO PARA DETECÇÃO DE FORMOL EM PRODUTOS LÁCTEOS E PRODUTOS DE HIGIENE PESSOAL
Due to its low cost, formaldehyde was commonly used as conservative in hygienic and cosmetic products. However, formaldehyde is strongly reactive and toxic, so its use has been forbidden in several products. Recently, it was reported by different media that formaldehyde has been added illegally in milk, causing innumerous health problems. In this work we dev
Quím. Nova. Publicado em: 2017-09
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2. An original method for producing acetaldehyde and diacetyl by yeast fermentation
Abstract In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde. The presence of whey powder and sodium citrate in the medium along with manganese and magnesium sulfate enhanced both biomas
Braz. J. Microbiol.. Publicado em: 2016-12
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3. An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive react
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-10
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4. Glycine reductase selenoprotein A is not a glycoprotein: the positive periodic acid-Schiff reagent test is the result of peptide bond cleavage and carbonyl group generation.
The complete amino acid sequence of Clostridium sticklandii selenoprotein A, a selenocysteine-containing protein component of the glycine reductase complex, has been established. Both the intact protein and peptide fragments produced by Staphylococcus aureus V8 protease or trypsin were purified by reversed-phase high-performance liquid chromatography and sub
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5. Reaction of glycolaldehyde with proteins: latent crosslinking potential of alpha-hydroxyaldehydes.
The Schiff base adducts of glyceraldehyde with hemoglobin undergo Amadori rearrangement to form stable ketoamine structures; this reaction is similar to the nonenzymic glucosylation of proteins. In the present studies the analogous rearrangement of the Schiff base adducts of glycolaldehyde with proteins has been demonstrated. However, the Amadori rearrangeme
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6. Cultural method for large-scale screening for Chlamydia trachomatis genital infection.
Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be
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7. The reaction of hydroxylamine with bacteriorhodopsin studied with mutants that have altered photocycles: selective reactivity of different photointermediates.
The reaction of the retinylidene Schiff base in bacteriorhodopsin (bR) to the water-soluble reagent hydroxylamine is enhanced by greater than 2 orders of magnitude under illumination. We have used this reaction as a probe for changes in Schiff base reactivity during the photocycle of wild-type bR and mutants defective in proton transport. We report here that
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8. The use of histological techniques for the demonstration of ion exchange resins.
AIM: To establish the staining characteristics of certain ion exchange resins in histological material, with a view to enabling confident differential identification. METHODS: Various histological staining procedures were applied to selected pathological material and prepared agar blocks containing the cation exchange resin calcium polystyrene sulphonate and
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9. Rosettes from Friend leukemia virus envelope: preparation and physicochemical and partial biological characterization.
Rosette-shaped particles mainly containing gp85 were isolated from Friend leukemia virus. The isolation procedure comprised lysis of the virion by Triton X-100, affinity chromatography on concanavalin A-Sepharose, and velocity sedimentation. The rosettes displayed a mean sedimentation constant of 32S and a buoyant density of 1.21 g/ml. They contained 1% Trit
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10. Histochemistry of the striated musculature in the opossum and human oesophagus.
The striated muscle component of the opossum oesophagus has been studied for fibre type as revealed by histochemical stains for succinic acid dehydrogenase, adenosine triphosphatase, nicotinamide adenine dinucleotide dehydrogenase and after staining with the periodic acid-Schiff reagent. The reactions were compared to those obtained in a single human oesopha
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11. Isolation of a glycoprotein from Mycoplasma pneumoniae membranes.
A glycoprotein was detected in Mycoplasma pneumoniae membranes. Its apparent molecular weight was about 60,000, as observed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. It corresponded to the single band that was detected on the gels by the carbohydrate stain, periodic acid-Schiff reagent. The intensity of this stained ban
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12. Caulobacter crescentus pili: structure and stage-specific expression.
Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cell; this occurs with the loss of flagella