Selection Of The Target Sequence
Mostrando 1-12 de 242 artigos, teses e dissertações.
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1. HSF1 and HSPA6 as functional candidate genes associated with heat tolerance in Angus cattle
ABSTRACT The purpose of this study was to access and characterize single nucleotide polymorphisms (SNP) located within the HSF1 and HSPA6 candidate genes for adaptability in Angus breed raised in subtropical climate. Samples of DNA from 20 animals representing extreme phenotypes for adaptability traits were obtained. Sequence variations in the candidate gene
R. Bras. Zootec.. Publicado em: 05/03/2018
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2. Variabilidade comportamental e a seleção de uma sequência de baixa probabilidade inicial: comparando dois procedimentos
O objetivo do estudo foi avaliar a efetividade de dois procedimentos, Tentativa Discreta (TD) e Tentativa Discreta com Intervalo entre as Respostas (TD/IRI), na produção da variabilidade comportamental em diferentes condições (diretamente reforçada e induzida) e averiguar que condição, em cada procedimento, mais facilitaria a seleção de uma sequênc
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 25/05/2012
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3. HLA-A, B and DRB1 allele and haplotype frequencies in volunteer bone marrow donors from the north of Parana state
BACKGROUND: Knowledge of allele and haplotype frequencies of the human leukocyte antigen (HLA) system is important in the search for unrelated bone marrow donors. The Brazilian population is very heterogeneous and the HLA system is highly informative of populations because of the high level of polymorphisms. AIM: The aim of this study was to characterize the
Revista Brasileira de Hematologia e Hemoterapia. Publicado em: 2012
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4. Aspectos de física estatística na evolução e no crescimento molecular. / Aspects of statistical physics on evolution and in molecular growth.
Molecular Evolution, stimulated and supported by Evolution Synthetic Theory, became essential to understand evolution of life. Genomic growth was responsible to increase the capacity of storage information and to stability of the molecule; besides, it was also submitted to natural selection. Using amplification-mutation-selection methodology, such as SELEX (
Publicado em: 2009
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5. Seleção e caracterização de peptídeos recombinantes ligantes a anticorpos monoclonais reativos a proteínas de Anaplasma marginale
Bovine anaplasmosis is caused by Anaplasma marginale and A. centrale. The most pathogenic and important species for cattle production is A. marginale, and is widely distributed in tropical, subtropical and temperate regions of the world. A. marginale is an intra-erythrocyte rickettsia of susceptible ruminants, biological and mechanically transmitted by ticks
Publicado em: 2008
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6. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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7. Identificação e caracterização de transcritos associados ao desenvolvimento e crescimento da musculatura esquelética em frangos (Gallus gallus) / Identification and characterization of transcripts associated with skeletal muscle development and growth in chicken (Gallus gallus)
by animal breeding programs along the years based on the observation of adult phenotypic characters. But, the number of myogenic precursor cells and, consequently, the number of muscle fibers is determined during the embryonic stages of development. Therefore, the embryonic myogenic program is an ideal target for searching potential candidate-transcripts, th
Publicado em: 2006
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8. Direct selection for sequences encoding proteases of known specificity.
We have developed a simple genetic selection that could be used to isolate eukaryotic cDNAs encoding proteases that cleave within a defined amino acid sequence. The selection was developed by using the transcription factor GAL4 from Saccharomyces cerevisiae as a selectable marker, a cloned protease from tobacco etch virus (TEV), and an 18-amino acid TEV prot
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9. Tn5/IS50 target recognition
This communication reports an analysis of Tn5/IS50 target site selection by using an extensive collection of Tn5 and IS50 insertions in two relatively small regions of DNA (less than 1 kb each). For both regions data were collected resulting from in vitro and in vivo transposition events. Since the data sets are consistent and transposase was the only protei
The National Academy of Sciences.
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10. In vitro selection of molecular beacons
While molecular beacons are primarily known as biosensors for the detection of nucleic acids, it has proven possible to adapt other nucleic acid binding species (aptamers) to function in a manner similar to molecular beacons, yielding fluorescent signals only in the presence of a cognate ligand. Unfortunately, engineering aptamer beacons requires a detailed
Oxford University Press.
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11. Directed integration of viral DNA mediated by fusion proteins consisting of human immunodeficiency virus type 1 integrase and Escherichia coli LexA protein.
We tested whether the selection of target sites can be manipulated by fusing retroviral integrase with a sequence-specific DNA-binding protein. A hybrid protein that has the Escherichia coli LexA protein fused to the C terminus of the human immunodeficiency virus type 1 integrase was constructed. The fusion protein, IN1-288/LA, retained the catalytic activit
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12. A phylogenetic approach to target selection for structural genomics: solution structure of YciH.
Structural genomics presents an enormous challenge with up to 100 000 protein targets in the human genome alone. At current rates of structure deter-mination, judicious selection of targets is necessary. Here, a phylogenetic approach to target selection is described which makes use of the National Center for Biotechnology Information database of Clusters of