Shell Vial
Mostrando 1-12 de 114 artigos, teses e dissertações.
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1. Comparison of a modified shell vial culture procedure with conventional mouse inoculation for rabies virus isolation
Rabies is a neurotropic disease that is often lethal. The early diagnosis of rabies infection is important and requires methods that allow for the isolation of the virus from animals and humans. The present study compared a modified shell vial (MSV) procedure using 24-well tissue culture plates with the mouse inoculation test (MIT), which is considered the g
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-04
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2. Survey of Rickettsia spp. in Amblyomma dubitatum Neumann 1899 and Amblyomma triste Koch 1844 ticks from Brazil and Uruguay, respectivily / Pesquisa de Rickettsia spp. em carrapatos Amblyomma dubitatum Neumann 1899 e Amblyomma triste Koch 1844, provenientes do Brasil e Uruguai, respectivamente
Owing to the potential role of the tick Amblyomma dubitatum and Amblyomma triste in the transmission of the Spotted Fever Group (SFG) Rickettsia, this study evaluated infection by Rickettsia in ticks collected in Brazil and Uruguay, where rickettsial infection is endemic. A total of 841 (367 males e 474 females) A. dubitatum adult ticks were collected in Ped
Publicado em: 2007
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3. Investigation on the Rickettsia parkeri infection in ticks Amblyomma triste in the state of São Paulo: isolation and molecular characterization of the bacterium / Investigação da infecção pela bactéria Rickettsia parkeri em carrapatos Amblyomma triste no Estado de São Paulo: isolamento e caracterização molecular da bactéria
Em janeiro de 2005, foram coletados 31 carrapatos adultos da espécie Amblyomma triste em uma propriedade rural da CESP, localizada no município de Paulicéia, Estado de São Paulo. Três carrapatos foram positivos para o teste de hemolinfa, demonstrando estruturas compatíveis com riquétsias no interior de hemócitos. Dois desses carrapatos foram submetid
Publicado em: 2006
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4. Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB
Memórias do Instituto Oswaldo Cruz. Publicado em: 2005-05
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5. Isolamento e detecção molecular de riquétsias do Grupo Febre Maculosa, a partir de Amblyomma cajennense (Fabricius, 1787) e espécimens biológicos humanos, provenientes de áreas endêmicas do Estado de São Paulo / SPOTTED FEVER GROUP RICKETTSIAE ISOLATION AND MOLECULAR DETECTION, FROM Amblyomma cajennense (FABRICIUS, 1787) AND HUMAN, FROM ENDEMIC AREAS OF STATE OF SÃO PAULO.
Brazilian spotted fever (BSF), a disease caused by spotted fever group rickettsiae (SFGR) transmitted by Amblyomma cajennense, is generally fatal when not early treated. Common laboratory methods (Vero cell culture and immunofluorescence indirect assay) are not efficient on the diagnosis in most of the cases observed in State of São Paulo, where the agent i
Publicado em: 2003
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6. Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.
Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of
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7. Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique.
A shell vial technique was used to recover respiratory syncytial virus (RSV) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. With specimens determined to be positive by direct fluorescence assay (DFA), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). The majority of RSV iso
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8. Diagnosis of cytomegalovirus infections by shell vial assay and conventional cell culture during antiviral prophylaxis.
A total of 3,552 specimens for conventional cytomegalovirus (CMV) culture and shell vial assay for CMV immediate-early antigen were obtained during a prospective randomized trial for prophylaxis of CMV disease after liver transplantation. Prophylaxis with ganciclovir for 2 weeks and then high-dose acyclovir for 2.5 months was compared with high-dose acyclovi
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9. Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection.
Specimens submitted for the diagnosis of cytomegalovirus (CMV) infection were inoculated into three (blood) or two (urine, tissue, bronchoalveolar lavage [BAL]) shell vials seeded with MRC-5 cells for the diagnosis of CMV infection. We evaluated the detection of 993 specimens that were positive for CMV according to the number of shell vial cell cultures inoc
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10. Isolation of Rickettsia prowazekii from Blood by Shell Vial Cell Culture
A blood sample from a patient who returned from Algeria with a fever inoculated on human embryonic lung fibroblasts by the shell vial cell culture technique led to the recovery of Rickettsia prowazekii. The last clinical strain was isolated 30 years ago. Shell vial cell culture is a versatile method that could replace the classic animal and/or embryonated eg
American Society for Microbiology.
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11. Rapid diagnosis of respiratory viral infections by using a shell vial assay and monoclonal antibody pool.
We compared the detection of seven respiratory viruses by using a commercially available monoclonal antibody pool in a 2-day shell vial assay with that by using standard cell culture with respiratory syncytial virus (RSV) enzyme-linked immunosorbent assay (ELISA)-negative nasal secretions from hospitalized children. We found 179 respiratory virus isolates by
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12. Detection of cytomegalovirus in shell vial cultures by using a DNA probe and early nuclear antigen monoclonal antibody.
An in situ biotinylated DNA probe assay was evaluated as an adjunct to anti-cytomegalovirus early nuclear antigen indirect immunofluorescence and cytopathic effect on cytomegalovirus-infected monolayers in shell vial cultures. Viral infection was detected by early nuclear antigen indirect immunofluorescence at 24 h and by DNA probe assay and shell vial cytop