Sirnas
Mostrando 1-12 de 180 artigos, teses e dissertações.
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1. Long non-coding RNA HOTAIR induces the PI3K/AKT/mTOR signaling pathway in breast cancer cells
SUMMARY OBJECTIVE: The phosphoinositide 3-kinase/protein kinase AKT/mammalian target of rapamycin signaling pathway is essential for proper cellular metabolism and cell growth. However, aberrant activation of this pathway has been linked to the progression and metastasis of breast cancer. Recently, the role of long non-coding RNAs in interfering with the ce
Revista da Associação Médica Brasileira. Publicado em: 2022
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2. Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus v
Braz. J. Microbiol.. Publicado em: 2017-07
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3. Effect of microRNA-21 on the proliferation of human degenerated nucleus pulposus by targeting programmed cell death 4
This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP ce
Braz J Med Biol Res. Publicado em: 24/05/2016
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4. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplex virus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three different small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene (sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large s
Braz J Infect Dis. Publicado em: 2014-08
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5. The new world of RNAs
One of the major developments that resulted from the human genome sequencing projects was a better understanding of the role of non-coding RNAs (ncRNAs). NcRNAs are divided into several different categories according to size and function; however, one shared feature is that they are not translated into proteins. In this review, we will discuss relevant aspec
Genet. Mol. Biol.. Publicado em: 2014
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6. In vitro and in vivo inhibition of rabies virus replication by RNA interference
Rabies is a zoonotic disease that affects all mammals and leads to more than 55,000 human deaths every year, caused by rabies virus (RABV) (Mononegavirales: Rhabdoviridae: Lyssavirus). Currently, human rabies treatment is based on the Milwaukee Protocol which consists on the induction of coma and massive antiviral therapy. The aim of this study was to assess
Braz. J. Microbiol.. Publicado em: 15/11/2013
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7. Desenvolvimento e caracterização de sistemas de liberação tópica a base de cristais líquidos para veiculação de siRNA na terapia gênica / Development and characterization of topical delivery systems based on liquid crystals for siRNA in gene therapy
A terapia gênica por interferência de RNA (RNAi) trata-se de um processo de silenciamento pós-transcricional capaz de suprimir a expressão de um determinado gene. A RNAi é uma proposta terapêutica promissora para o tratamento de muitas doenças severas que ainda não possuem cura ou terapias bem definidas. Porém, é necessário o desenvolvimento de si
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/05/2012
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8. Analysis of energetically biased transcripts of viruses and transposable elements
RNA interference (RNAi) is a natural endogenous process by which double-stranded RNA molecules trigger potent and specific gene silencing in eukaryotic cells and is characterized by target RNA cleavage. In mammals, small interfering RNAs (siRNAs) are the trigger molecules of choice and constitute a new class of RNA-based antiviral agents. In an efficient RNA
Genet. Mol. Biol.. Publicado em: 13/11/2012
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9. Metatranscriptomic analysis of small RNAs present in soybean deep sequencing libraries
A large number of small RNAs unrelated to the soybean genome were identified after deep sequencing of soybean small RNA libraries. A metatranscriptomic analysis was carried out to identify the origin of these sequences. Comparative analyses of small interference RNAs (siRNAs) present in samples collected in open areas corresponding to soybean field plantatio
Genetics and Molecular Biology. Publicado em: 2012
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10. The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis
Searching for effective Smad3 gene-based gene therapies for hepatic fibrosis, we constructed siRNA expression plasmids targeting the rat Smad3 gene and then delivered these plasmids into hepatic stellate cells (HSCs). The effect of siRNAs on the mRNA levels of Smad2, Smad3, Smad4, and collagens I-α1, III-α1 and IV-α1 (Colα1, Col3α1, Col4α1, respectivel
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-02
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11. Inibição da replicação do virus da raiva in vitro e in vivo por meio de interferência por RNA / Inhibition of replication of rabies virus in vivo and in vitro using RNA interference
A raiva é uma zoonose que afeta todos os mamíferos e causa cerca de 55.000 mortes humanas por ano, causada pelo vírus da raiva. O vírus da raiva pertence à Ordem Mononegavirales, Família Rhabdoviridae e o Gênero Lissavirus. Atualmente, o tratamento humano se baseia no uso do Protocolo de Milwaukee composto de indução do paciente ao coma e uso de mas
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/08/2010
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12. O papel do interferon do tipo I e sua sinalização na resposta imune inata contra a infecção pela Brucella abortus
Brucella abortus é uma bactéria Gram-negativa, patógeno intracelular facultativo, que causa febre ondulante e artrite em humanos e infertilidade em animais, resultando em sérias perdas econômicas. O reconhecimento de Brucella por PRRs, como os TLR2, TLR4 e TLR9, via molécula adaptadora MyD88, é importante para o estabelecimento de uma resposta imune e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/03/2010