Smgt
Mostrando 1-6 de 6 artigos, teses e dissertações.
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1. Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental / In situ hybridization in bovine sperm treated with exogenous DNA: an experimental study.
Muitas das técnicas utilizadas para gerar animais transgênicos são caras e laboriosas. Neste contexto, a transferência gênica mediada por espermatozóides (TGME) pode ser uma alternativa para a produção em larga escala de animais transgênicos. Estudos de SMGT têm seu foco no número de cópias de DNA incorporada pelo espermatozóide. Por isso, há p
Publicado em: 2010
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2. Genic transfer in sperm cells in Mus musculus and Ramdia quelem / Transferência gênica em células espermáticas de Mus musculus e Ramdia quelen
Transgenic animals have been used as biological models in studies of the genes functions and their mechanisms of action, as well as to improve animal production. Researchers are trying to produce transgenic animals that will be organs donors in xenotransplants. Another use of the transgenic animal is in the production of recombinant proteins for pharmaceutic
Publicado em: 2009
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3. Systematic head and neck physical examination as a predictor of obstructive sleep apnea in class III obese patients
Our aim was to determine if anatomical abnormalities of the upper airway (UA) and facial skeleton of class III severely obese patients are related to the presence and severity of obstructive sleep apnea syndrome (OSAS). Forty-five patients (69% females, mean age 46.5 ± 10.8 years) with a body mass index (BMI) over 40 kg/m² underwent UA and facial skeletal
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-12
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4. Caracterização, criopreservação e manipulação genética do sêmen do linguado Paralichthys orbignyanus (Teleostei: Paralichthyidae).
The Brazilian flounder Paralichthys orbignyanus is an important fishing resource from southern Atlantic coast of America and it has being considered for aquaculture. The sperm quality is an important variable in reproductive management of cultured species, influencing directly on the production of viable eggs. Moreover, the adequate knowledge of physical and
Publicado em: 2008
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5. Transferência gênica mediada por espermatozóides para geração de jundiás (Rhamdia quelen) transgênicos / Sperm Mediated Gene Transfer for generation of transgenic jundiás (Rhamdia quelen)
Various methodologies to insert exogenous DNA into eukaryotic cells have been studied in the last two decades. In fish, the technique most studied to the moment is microinjection, which promotes the insertion of fragments of interest genes into zygotes. However, this methodology has some negative aspects: it is time and work consuming, requires qualified lab
Publicado em: 2004
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6. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation
A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying th
National Academy of Sciences.