Sos Chromotest
Mostrando 1-8 de 8 artigos, teses e dissertações.
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1. Isolation and characterization of flavanols from Anthocephalus cadamba and evaluation of their antioxidant, antigenotoxic, cytotoxic and COX-2 inhibitory activities
ABSTRACT In search of lead molecules for use in disease prevention and as food additive from natural sources, two flavanols were isolated from leaves of Anthocephalus cadamba (Roxb.) Miq., Rubiaceae. Their structures were established as 6-hydroxycoumarin-(4"→8)-(-)-epicatechin and 6-hydroxycoumarin-(4"→8)-(-)-epicatechin-(4→6‴)-(-)-epicatechin on the
Rev. bras. farmacogn.. Publicado em: 2016-08
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2. The influence of organic solvents on estimates of genotoxicity and antigenotoxicity in the SOS chromotest
In this work, the toxicity and genotoxicity of organic solvents (acetone, carbon tetrachloride, dichloromethane, dimethylsulfoxide, ethanol, ether and methanol) were studied using the SOS chromotest. The influence of these solvents on the direct genotoxicity induced by the mutagens mitomycin C (MMC) and 4-nitroquinoline-1-oxide (4-NQO) were also investigated
Genetics and Molecular Biology. Publicado em: 2012
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3. Chemical composition and antigenotoxic properties of Lippia alba essential oils
The present work evaluated the chemical composition and the DNA protective effect of the essential oils (EOs) from Lippia alba against bleomycin-induced genotoxicity. EO constituents were determined by Gas Chromatography/Mass Spectrometric (GC-MS) analysis. The major compounds encountered being citral (33% geranial and 25% neral), geraniol (7%) and trans-β-
Genetics and Molecular Biology. Publicado em: 29/07/2011
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4. Genotoxicity and mutagenicity of Echinodorus macrophyllus (chapéu-de-couro) extracts
Echinodorus macrophyllus, commonly known as chapéu-de-couro, is a medicinal plant used in folk medicine to treat inflammation and rheumatic diseases. In this work, we used short-term bacterial assays based on the induction of SOS functions to examine the genotoxicity and mutagenicity of an aqueous extract of E. macrophyllus leaves. Whole extract and an ethy
Genetics and Molecular Biology. Publicado em: 02/07/2010
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5. Genotoxic effect of alkaloids
Because of the increased use of alkaloids in general medical practice in recent years, it is of interest to determine genotoxic, mutagenic and recombinogenic response to different groups of alkaloids in prokaryotic and eucaryotic organisms. Reserpine, boldine and chelerythrine did not show genotoxicity response in the SOS-Chromotest whereas skimmianine showe
Publicado em: 2010
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6. Genotoxicidade e mutagenicidade diretas de substâncias e materiais endodônticos avaliadas por dois testes procariotas
Substâncias e materiais utilizados na terapia endodôntica entram em íntimo contato com os tecidos perirradiculares via forame apical e foraminas e, em decorrência disto, deveriam idealmente possuir as seguintes características: biocompatibilidade e ausência de mutagenicidade. Existem poucos estudos avaliando o potencial genotóxico e mutagênico de sub
Journal of Applied Oral Science. Publicado em: 2005-12
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7. SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity.
We present and evaluate the SOS chromotest, a bacterial test for detecting DNA-damaging agents. It is a colorimetric assay based on the induction by these agents of the SOS function sfiA, whose level of expression is monitored by means of a sfiA::lacZ operon fusion. The response is rapid (a few hours), and does not require survival of the tester strain. Dose
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8. A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells.
A genetically controlled luminescent bacterial reporter assay, the SOS lux test, was developed for rapid detection of environmental genotoxins. The bioassay is based on the recombinant plasmid pPLS-1, which was constructed as a derivative of pBR322, carrying the promoterless luxCDABFE genes of Photobacterium leiognathi downstream of a truncated cda gene from