Spiroplasma Citri
Mostrando 1-12 de 52 artigos, teses e dissertações.
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1. Spiroplasma citri, praga quarentenária A1 para o Brasil: aspectos gerais e impacto econômico.
Expressão econômica; Distribuição geográfica; Sintomas; Bioecologia; Epidemiologia; Detecção e diagnóstico; Medidas quarentenárias e de contingência.
Brasília: Embrapa Recursos Genéticos e Biotecnologia. Publicado em: 2011
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2. Construção de vetor oriC de Mycoplasma hyopneumoniae uma ferramenta para estudos genéticos do agente da pneumonia enzoótica suína.
Mycoplasma hyopneumoniae é o agente etiológico da pneumonia enzoótica, enfermidade distribuída mundialmente em rebanhos suínos nas fases de crescimento e terminação. A pneumonia enzoótica tem sido considerada uma das mais importantes causas de perdas econômicas na suinocultura mundial. Tendo em vista a disponibilidade dos genomas completos de três
Publicado em: 2008
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3. A third DNA polymerase from Spiroplasma citri and two other spiroplasmas.
Recently, two DNA polymerases (ScA and ScB) were isolated and characterized from Spiroplasma citri. We now have found a third DNA polymerase (ScC) not only in S. citri but also in the serologically related honeybee spiroplasma BC3 and the unrelated flower spiroplasma BNR1. Enzyme ScC is N-ethylmaleimide (NEM) sensitive. The three DNA polymerases from the hon
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4. Comparison of lipids from Spiroplasma citri and corn stunt spiroplasma.
The qualitative lipid composition of Spiroplasma citri and corn stunt spiroplasma is identical. Small amounts of acylated glucose and steryl glucoside were found.
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5. Comparison of the membrane composition of Spiroplasma citri and the corn stunt Spiroplasma.
Components of membranes isolated from Spiroplasma citri and corn stunt spiroplasma grown at 28 degrees C were analyzed. On a protein basis, lipid phosphorus was lower and cholesterol was higher in S. citri. Only minor differences between the two species were found in fatty acid composition, reduced nicotinamide adenine dinucleotide diaphorase, and adenosine
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6. A physical and genetic map of the Spiroplasma citri genome.
A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis. A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to 1780 kbp, the largest of all Moll
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7. Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum.
In previous studies (A. Marais, J. M. Bove, and J. Renaudin, J. Bacteriol. 178:862-870, 1996), we have shown that the recA gene of Spiroplasma citri R8A2 was restricted to the first 390 nucleotides of the N-terminal part. PCR amplification and sequencing studies of five additional strains of S. citri have revealed that these strains had the same organization
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8. Antigenic relatedness between the spiralins of Spiroplasma citri and Spiroplasma melliferum.
Four spiralins were compared by rocket immunoelectrophoresis, quantitative immunoblotting techniques, and the spiroplasma deformation test with the use of antispiralin (polyclonal) monospecific antibodies. This investigation revealed that the spiralins of Spiroplasma citri and S. melliferum are antigenically related and that probably no more than two epitope
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9. Integrative and free Spiroplasma citri oriC plasmids: expression of the Spiroplasma phoeniceum spiralin in Spiroplasma citri.
The replication region (oriC) of the Spiroplasma citri chromosome has been recently sequenced, and a 2-kbp DNA fragment was characterized as an autonomously replicating sequence (F. Ye, J. Renaudin, J. M. Bové, and F. Laigret, Curr. Microbiol. 29:23-29, 1994). In the present studies, we have combined this DNA fragment, containing the dnaA gene and the flank
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10. Growth and division of Spiroplasma citri: elongation of elementary helices.
The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods of membrane labeling. When labeling is don
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11. Chemically Defined Medium for Cultivation of Several Epiphytic and Phytopathogenic Spiroplasmas
A chemically defined medium, LD82, was formulated for in vitro cultivation of spiroplasmas. Medium LD82 supported good growth for four epiphytic and insect-pathogenic spiroplasmas, Spiroplasma floricola 23-6T, Spiroplasma sp. strain SR3, Spiroplasma sp. strain brevi, and Spiroplasma sp. strain AS576, and of the phytopathogenic spiroplasmas Spiroplasma citri
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12. Filter Paper Dot-Immunobinding Assay for Detection of Spiroplasma citri
A rapid filter paper dot-immunobinding assay was adapted to detect the wall-less mollicute Spiroplasma citri in medium, plants, or insects. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus hydrogen peroxide. The detection limit averaged 2.3 × 1010 C