Spleen Aspirates
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method
Memórias do Instituto Oswaldo Cruz. Publicado em: 2010-05
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2. Cutaneous leishmaniais in the state of CearÃ: Historical and clinical aspects and therapeutic evolution. / Leishmaniose cutÃnea no estado do CearÃ: Aspectos histÃricos, clÃnicos e evoluÃÃo terapÃutica.
Cutaneous leishmaniasis is an important health problem in Brazil.The disease has spread to all regions and autochtonous cases have been reported from all states. The total number of cases reported in the country from 1980 to 2005, was 613, 644. In CearÃ, cutaneous leishmaniasis is an endemic disease in all regions of the state and in many municipalities hun
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/12/2009
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3. Comparison of conventional methods for diagnosis of visceral leishmaniasis in children of the Center-West Region of Brazil
In Brazil, sophisticated techniques currently employed for diagnosis of visceral leishmaniasis, such as polymerase chain reaction-based assays, are only available in major research centers, whereas conventional methods are still used in many areas where the disease occurs. In the state of Mato Grosso do Sul, in the country's Center-West Region, visceral leis
Brazilian Journal of Infectious Diseases. Publicado em: 2007-02
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4. Leishmaniose Visceral em pacientes infectados por HIV. Estudo de casos observados em Campo Grande, MS, 2000-2006. / Leishmaniasis Visceral in patients infected by HIV. Case studies observed in Campo Grande, MS, 2000-2006.
The outspread and urbanization of visceral leishmaniasis in Mato Grosso do Sul becoming the disease a public health problem in the state. To know the clinical and epidemiological characteristics of visceral leishmaniasis in HIV patients, a series of 23 patients attended in Campo Grande, center west region of Brazil, from 2000 to 2006, was studied. We selecte
Publicado em: 2007
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5. Método para análise de aspirado esplênico de cão: desenvolvimento de ferramentas aplicáveis ao monitoramento de variações de populações leucocitárias na Leishmaniose visceral. / A method for the analysis of fine-needle aspirates of canine spleen: development of tools for the monitoring of leukocyte population chages in visceral leishmaniosis.
Introduction: This study was focused on the development of a method for the analysis of the canine spleen as a target organ for diagnostic evaluation, aiming to characterize cellular components of the in situ immune response to infection by Leishmania chagasi, since the dog stands as reservoir of the parasite in endemic areas of the disease. Methods: The stu
Publicado em: 2005
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6. Amplification of Ehrlichial DNA from Dogs 34 Months after Infection with Ehrlichia canis
In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from
American Society for Microbiology.
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7. Diagnosis of Kala-Azar by Nested PCR Based on Amplification of the Leishmania Mini-Exon Gene
To diagnose visceral leishmaniasis (kala-azar), we have developed a nested PCR method based on amplification of the mini-exon gene, which is unique and tandomly repeated in the Leishmania genome. Nested PCR was sufficiently sensitive for the detection of DNA in an amount equivalent to a single Leishmania parasite or less. We examined the usefulness of this P
American Society for Microbiology.
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8. Granuloma formation in patients receiving BCG immunotherapy.
Eleven patients with acute myeloblastic leukaemia have received repeated intravenous injections of BCG containing 4-9 X 10(6) live organisms per millilitre. Non-caseating epithelioid granulomas, sometimes with giant-cell formation, have been demonstrated in eight bone marrow aspirates. Seven patients had granulomas in the liver, three in the lung, one in the
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9. Mechanisms of neutropenia involving myeloid maturation arrest in burn sepsis.
OBJECTIVE: To determine the mechanisms that lead to the decrease in bone marrow production of neutrophils during burn sepsis. SUMMARY BACKGROUND DATA: Impaired bone marrow granulopoiesis during burn sepsis often results in neutropenia despite elevated circulating levels of granulocyte colony-stimulating factor (G-CSF). To date, neither the specific stages of
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10. Development of a Species-Specific PCR Assay for Detection of Leishmania donovani in Clinical Samples from Patients with Kala-Azar and Post-Kala-Azar Dermal Leishmaniasis
We have developed a PCR assay that is capable of amplifying kinetoplast DNA (kDNA) of Leishmania donovani in a species-specific manner among Old World leishmanias. With Indian strains and isolates of L. donovani the assay was sensitive enough to detect kDNA in an amount equivalent to a single parasite or less. The extreme sensitivity of the assay was reflect
American Society for Microbiology.
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11. Diagnostic relevance of peripheral blood immunocytochemistry in hairy cell leukaemia.
AIMS--(1) To assess the diagnostic relevance of peripheral blood immunocytochemistry in hairy cell leukaemia (HCL); (2) to compare the immunostaining of bone marrow biopsy specimens with bone marrow and peripheral blood cytospins; (3) to evaluate the sensitivity of the different markers used; (4) to identify the ultrastructural localisation of DBA.44 in HCL
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12. Pathophysiology of HIV related thrombocytopenia: an analysis of 41 patients.
AIM--To analyse the pathogenic mechanism of HIV related thrombocytopenia. METHODS--Forty one patients with thrombocytopenia and HIV-1 infection were investigated over two years. Anticardiolipin antibodies were measured using an enzyme linked immunosorbent assay and antiplatelet antibodies were measured using an immunocapture technique. Tests for VDRL, C3 and