Stachyose Synthase
Mostrando 1-12 de 14 artigos, teses e dissertações.
-
1. Effect of a mutation in Raffinose Synthase 2 (GmRS2 ) on soybean quality traits
Abstract The presence of stachyose and raffinose is considered an antinutritional factor for humans and monogastric animals, leading to limitations on soybean consumption as a protein source. In the present study, the effect of a mutation in the raffinose synthase 2 gene was measured on soybean quality traits. We used an F2 population with 168 soybean indivi
Crop Breed. Appl. Biotechnol.. Publicado em: 11/04/2019
-
2. Purificação e caracterização de uma α-galactosidase em sementes de Tachigali multijuga e clonagem parcial do gene da estaquiose sintase de soja / Purification and characterization of α-galactosidase from Tachigali multijuga seeds and partial cloning of soybean stachyose synthase gene
A soja, devido ao seu alto valor nutricional, é mundialmente consumida na forma de óleo, margarinas, proteína texturizada e extrato hidrossolúvel. O extrato hidrossolúvel, conhecido como leite de soja, é uma alternativa alimentar para muitas pessoas com intolerância à lactose. No entanto, a presença dos galactooligossacarídeos (GO) restringe o cons
Publicado em: 2007
-
3. Analysis of the Raffinose Family Oligosaccharide Pathway in Pea Seeds with Contrasting Carbohydrate Composition1
Raffinose family oligosaccharides (RFOs) are synthesized by a set of galactosyltransferases, which sequentially add galactose units from galactinol to sucrose. The accumulation of RFOs was studied in maturing seeds of two pea (Pisum sativum) lines with contrasting RFO composition. Seeds of the line SD1 accumulated stachyose as the predominant RFO, whereas ve
American Society of Plant Physiologists.
-
4. Stachyose Synthesis in Source Leaf Tissues of the CAM Plant Xerosicyos danguyi H. Humb. 1
Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated from leaf tissues, and the extractable activ
-
5. Galactosylononitol and Stachyose Synthesis in Seeds of Adzuki Bean1 : Purification and Characterization of Stachyose Synthase
Stachyose synthase (STS) (EC 2.4.1.67) was purified to homogeneity from mature seeds of adzuki bean (Vigna angularis). Electrophoresis under denaturing conditions revealed a single polypeptide of 90 kD. Size-exclusion chromatography of the purified enzyme yielded two activity peaks with apparent molecular masses of 110 and 283 kD. By isoelectric focusin
American Society of Plant Physiologists.
-
6. Galactinol Synthase Activity and Soluble Sugars in Developing Seeds of Four Soybean Genotypes 1
Galactinol synthase (UDP-galactose:inositol galactosyltransferase) is the first unique enzyme in the biosynthetic pathway of raffinose saccharides. Its role as a regulator of carbon partitioning between sucrose and raffinose saccharides in developing soybean (Glycine max L. Merrill) seeds was examined. Galactinol synthase activity and concentrations of sucro
-
7. Metabolism of the Raffinose Family Oligosaccharides in Leaves of Ajuga reptans L. (Inter- and Intracellular Compartmentation).
We recently suggested that leaves of the frost-hardy species Ajuga reptans L. (Lamiaceace) contain two pools of raffinose family oligosaccharides (RFO): a large long-term storage pool in the mesophyll, possibly also involved in frost resistance, and a transport pool in the phloem (M. Bachmann, P. Matile, F. Keller [1994] Plant Physiol 105: 1335-1345). In the
-
8. A Potential Pathway for Galactose Metabolism in Cucumis sativus L., A Stachyose Transporting Species 1
Conversion of [14C]galactose (Gal) 1-P, UDP-[14C]Gal, or UDP-[14C]glucose to [14C]sucrose was observed when cell-free homogenates of cucumber (Cucumis sativus L.) fruit peduncles were incubated with individual 14C-labeled substrates, appropriate cofactors, and fructose. The sucrose product was labeled only in the glucose moiety. Conversion of [14C]Gal-1-P to
-
9. Genetic Analysis of Seed-Soluble Oligosaccharides in Relation to Seed Storability of Arabidopsis1
Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance and consequently in seed storability. In the present work we analyzed the seed-soluble OS (sucrose, raffinose, and stachyose) content of several Arabidopsis accessions and thus identified the genot
American Society of Plant Physiologists.
-
10. Regulation of Photosynthetic Carbon Metabolism in Cucumber by Light Intensity and Photosynthetic Period 1
The effects of photosynthetic periods and light intensity on cucumber (Cucumis sativus L.) carbon exchange rates and photoassimilate partitioning were determined in relation to the activities of galactinol synthase and sucrose-phosphate synthase. Carbon assimilation and partitioning appeared to be controlled by different mechanisms. Carbon exchange rates wer
-
11. Galactinol synthase1. A Novel Heat Shock Factor Target Gene Responsible for Heat-Induced Synthesis of Raffinose Family Oligosaccharides in Arabidopsis1[w]
Heat shock factors (HSFs) are transcriptional regulators of the heat shock response. The major target of HSFs are the genes encoding heat shock proteins (HSPs), which are known to have a protective function that counteracts cytotoxic effects. To identify other HSF target genes, which may be important determinants for the generation of stress tolerance in Ara
American Society of Plant Biologists.
-
12. Water Deficit Effects on Raffinose Family Oligosaccharide Metabolism in Coleus1
Variegated coleus (Coleus blumei Benth.) plants were exposed to a restricted water supply for 21 d. The relative water content in leaf tissues was reduced from 80% (control) to 60% (drought-stressed). Under drought conditions, the stomatal conductance and leaf photosynthetic rate were reduced. In green leaf tissues, drought stress also greatly decreased the
American Society of Plant Physiologists.