Subunits Dissociation
Mostrando 1-12 de 265 artigos, teses e dissertações.
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1. Inhibition mechanism of the nicotinic acetylcholine receptor α3β4 tacrine / Mecanismos de inibição do receptor nicotínico de acetilcolina α3β4 pela tacrina
Neuronal nicotinic acetylcholine (cholinergic) receptors (nAChRs) are integral membrane proteins and belong to the family of ligand-gated cation channels composed by α and β subunits. These receptors play a key role in the signal transmission between neurons in the central and peripheral nervous system. The α3β4 subtype, for example, is t
Publicado em: 2008
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2. Mechanism of association of Na,K-ATPase subunits studied by chemical and physical agents: comparison between solubilized and liposome reconstituted enzyme. / Mecanismos de dissociação das subunidades alfa e Beta da Na,K-ATPase por agentes químicos e físicos: comparação entre a enzima solubilizada e reconstituída em lipossomos
A Na,K-ATPase é uma proteína encontrada na membrana plasmática de praticamente todas as células animais, que utiliza a energia derivada da hidrólise do ATP para transportar 3 íons Na+ e 2 íons K+. É composta por duas subunidades denominadas e . Um aspecto que ainda gera controvérsias se refere à sua forma de associação nativa e funcional como um
Publicado em: 2007
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3. Construção e análise de mutantes fluorescentes da troponina I / Construction and analysis of fluorescent mutants of troponin I
Vertebrate striated muscle contraction is regulated by troponin (Tn). Tn is composed of three subunits: troponin I (TnI), troponin C (TnC) and troponin T (TnT). TnI has an inhibitory role that is neutralized by calcium binding to the regulatory sites in the N-domain of TnC, and TnT positions the troponin complex on the thin filament. In order to follow the C
Publicado em: 2001
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4. Analise da estrutura de piruvato descarboxilase de Saccharomyces cerevisiae MC 16
t: Pyruvate decarboxylase (PDC, EC 4.1.1.1) is a key enzyme in the yeast carbon metabolism, driving pyruvic acid towards ethanol production. Although it is well established that the enzyme is a tetramer of about 240 KD, its monomeric subunit structure is still a matter of controversy. Either one or two subunit types have been described and three structural g
Publicado em: 1993
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5. Mechanism of dissociation of ribosomes of Escherichia coli by initiation factor IF-3.
Initiation factor IF-3 clearly can cause dissociation of ribosomes by binding to 30S subunits and thus shifting the spontaneous equilibrium, but it theoretically may also do so by binding to 70S ribosomes. Previously reported experiments with differentially labeled ribosomes and subunits seemed to eliminate the latter mechanism, but these experiments neglect
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6. Factor-Promoted Dissociation of Free Ribosomes in a Rabbit Reticulocyte Lysate System: Inhibition and Requirement for an Energy Source
A factor that promoted the dissociation of ribosomes into ribosomal subunits in a complete globin-synthesizing system was isolated from rabbit reticulocyte ribosomes. The factor stimulated both globin synthesis and the association of the small ribosomal subunit with polyribosomes. The dissociation of free ribosomes by the factor could be followed as an indep
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7. Studies on Pea Ribosomal Proteins: Conformational and Biological Activity Changes of Ribosomal Subunits Derived by NH4Cl Dissociation 12
Ribosomal subunits prepared by NH4Cl dissociation (0.5 m) of the monomeric ribosomes were much less active in in vitro protein synthesis than those prepared by KCl dissociation. The decrease in activity correlated with a detachment of some proteins (L2 and L9 as shown by gel electrophoresis) within the 60S ribosomal subunits. Subunits prepared with 0.3 m NH4
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8. Pressure-Induced Dissociation of Sedimenting Ribosomes: Effect on Sedimentation Patterns
Anomalous sedimentation patterns arise when free ribosomes from sea urchin eggs are centrifuged at high speeds. Pressure-induced dissociation of the ribosomes during sedimentation can explain the peculiar behavior; the assumption of such dissociation also yields estimates of the equilibrium constant (as a function of KCl concentration) and the change in mole
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9. Dissociation of N2 Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea 1
The dissociation of N2 gas-induced monomeric ribosomes from the pea root was studied by varying the concentration of KCl (or NH4Cl) and MgCl2 in the presence of dithiothreitol. These monoribosomes were shown to dissociate completely into subunits at 0.5m KCl or NH4Cl in the presence of 5 mm MgCl2. The 40S subunits were more susceptible to structural change i
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10. Dissociation of Mammalian Polyribosomes into Subunits by Puromycin
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0°C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA.
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11. Dissociation of Ribosomes and Seed Germination 1
Ribosomes from rice embryos (Oryza sativa) were dissociated into ribosomal subunits in vitro by systematic reduction of the Mg2+ concentration. Ribosomes from imbibed (28 C) embryos were more easily dissociated than those from nonimbibed embryos. This was not observed with ribosomes from either imbibed, nonviable embryos, or from embryos imbibed at 0 C. Ribo
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12. Dissociation and reassociation of immobilized porphobilinogen synthase: use of immobilized subunits for enzyme isolation.
The dissociation and association of an immobilized preparation of the octameric enzyme porphobilinogen synthase [5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24] is described. On treatment of the immobilized preparation with 4 M urea, four subunits per octamer are removed which can be reassociated into a soluble octameric