Tdna Pcr
Mostrando 1-12 de 33 artigos, teses e dissertações.
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1. Genetic engineering of cotton with a novel cry2AX1 gene to impart insect resistance against Helicoverpa armigera
Abstract Embryogenic calli of cotton (Coker310) were cocultivated with the Agrobacterium tumefaciens strain LBA4404 harbouring the codon-optimised, chimeric cry2AX1 gene consisting of sequences from cry2Aa and cry2Ac genes isolated from Indian strains of Bacillus thuringiensis. Forty-eight putative transgenic plants were regenerated, and PCR analysis of thes
Crop Breed. Appl. Biotechnol.. Publicado em: 2016-09
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2. Vias de sinalização de estresses em plantas : análise da região promotora do gene NIMIN-1 de Arabidopsis thaliana e da proteína ScCBL1 de cana-de-açúcar (Saccharum spp.) / Signal transductional pathways under biotic and abiotic stress in plants : functional analysis of NIMIN-1 promoter region in Arabidopsis and characterization of a calcium binding protein (ScCBL1) in sugar cane Saccharum spp.)
Estresses bióticos e abióticos como a seca, salinidade e ataque por patógenos são responsáveis por perdas significantes em culturas de grãos ao redor do mundo. Diversos genes são regulados em resposta a esses fatores e podem ser ativados ou reprimidos para gerar uma resposta específica na planta de maneira a gerar uma resposta de defesa que atenue os
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/04/2010
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3. Identificação de genes associados a produção de ocratoxina A em Aspergillus westerdijkiae
Mycotoxins, secondary metabolites of fungi, are toxic compounds found in great variety of foods, and when ingested can be very hazardous to animal and human health. Among the diverse mycotoxins, special interest has been given to the ochratoxin A (OA), produced by some species of Aspergillus and Penicillium. This mycotoxin is frequently found mainly in food
Publicado em: 2009
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4. Identificação de genes expressos durante a fase pré-simbiótica da associação ectomicorrízica entre Hydnangium sp. e Eucalyptus grandis e transformação de fungos ectomicorrízicos / Identification of genes expressed during the pre-symbiotic interaction in the Hydnangium sp.-Eucalyptus grandis ectomycorrhizal association and transformation of ectomycorrhizal fungi
Gene expression in the ectomycorrhizal fungus Hydnangium sp. during the pre-symbiotic interaction was evaluated using an in vitro mycorrhization technique aiming at constructing a suppressive subtractive cDNA library. The fungus was cultivated in the presence of Eucalyptus grandis roots, with no direct physical contact between both organisms. Genes that code
Publicado em: 2008
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5. Genetic transformation of soybean cotyledonary node seeking the cosuppression of the lysophosphatidycholine acyltransferase / Transformação genética de nós cotiledonares de soja visando à co-supressão do gene da lisofosfatidilcolina aciltransferase
A soja (Glycine max L. Merrill) é uma das mais importantes culturas do mundo e a maior do Brasil em área plantada. O Brasil é o segundo maior produtor mundial desta leguminosa atingindo 56,3 milhões de toneladas na safra 2006/2007. (www.conab.gov.br). Assim, modificações usando técnicas de engenharia genética podem facilitar o rápido desenvolvimento
Publicado em: 2007
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6. Estudo de proteases degradadoras de cutícula produzidas por Beauveria bassiana
The entomopathogenic fungus Beauveria bassiana is considered a promising agent of microbial control of insects considered pests of agriculture, such as, the grasshopper Rhammatocerus schistocercoides. This grasshopper attacks economically important plantations in the center-west Brazil. B. bassiana is capable to penetrate through cuticle of the host by apres
Publicado em: 2007
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7. Polymorphism of the rDNA and tDNA loci in clinical isolates of Pseudomonas aeruginosa: a perspective for molecular epidemiology surveillance
The Gram-negative bacterium Pseudomonas aeruginosa has a wide environmental and ecological distribution. It is an opportunistic pathogen that acquires resistance to multiple antimicrobial agents and can infect plants, animals and humans. We used rDNA and tDNA PCR markers to characterize the bacterial diversity of P. aeruginosa strains isolated at a Brazilian
Genetics and Molecular Biology. Publicado em: 2006
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8. UtilizaÃÃo de marcadores de rdna-pcr e tdna-pcr para a tipagem de isolados clÃnicos de Pseudomonas aeruginosa
Pseudomonas aeruginosa à uma bacteria Gram-negativa ubÃqua e oportunista. Na rotina hospitalar, os marcadores fenotÃpicos nem sempre revelam a diversidade das bactÃrias distribuÃdas nos diversos setores, assim, aplicamos trÃs mÃtodos moleculares baseados na amplificaÃÃo por PCR do locus de rDNA e tDNA para caracterizar a diversidade genÃtica de lin
Publicado em: 2005
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9. A High-Throughput Arabidopsis Reverse Genetics System
A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from ∼100,000 transformed lines. A total of 85,108 TAIL-PCR produc
American Society of Plant Biologists.
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10. Rapid and accurate identification of Staphylococcus species by tRNA intergenic spacer length polymorphism analysis.
PCR-amplified tRNA gene (tDNA) intergenic spacer length polymorphism (tDNA-ILP) was analyzed for its ability to identify to the species level type strains (n = 18) and clinical isolates (n = 163) of staphylococci. Amplified products obtained by PCR with outwardly directed consensus tDNA primers were separated by agarose and polyacrylamide gel electrophoreses
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11. Application and Evaluation of the Interlaboratory Reproducibility of tRNA Intergenic Length Polymorphism Analysis (tDNA-PCR) for Identification of Streptococcus Species
The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different
American Society for Microbiology.
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12. Association of single-stranded transferred DNA from Agrobacterium tumefaciens with tobacco cells.
During the inception of crown gall tumorigenesis, the transferred DNA (T-DNA) is processed from the Ti (tumor inducing) plasmid of Agrobacterium tumefaciens and is transferred to plant cells. T-DNA processing and transfer require the induction of vir (virulence) genes by phenolic compounds secreted by wounded plant cells. After vir gene induction, both singl