Tertiary Protein Structures
Mostrando 1-12 de 127 artigos, teses e dissertações.
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1. Homology modeling and epitope prediction of Der f 33
Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cel
Braz J Med Biol Res. Publicado em: 15/03/2018
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2. Identification of a type I nitroreductase gene in non-virulent Trypanosoma rangeli
ABSTRACT Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) s
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-07
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3. Função de avaliação dinâmica em algoritmos genéticos aplicados na predição de estruturas tridimensionais de proteínas / Genetic Algorithms with Dynamic Fitness Functions Applied to Tridimensional Protein Structure Prediction
The protein structure prediction can be seen as an optimization problem where given an amino acid sequence, the tertiary protein structure must be found amongst many possible by obtaining energy functions minima. Many researchers have been proposing Evolutionary Computation strategies to find tridimensional structures of proteins; however results are not alw
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/09/2012
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4. Desenvolvimento de modelo multidimensional de predição de peptídeos antimicrobianos utilizando um sistema de inferência neuro-fuzzy adaptativo (ANFIS)
Antimicrobial peptides (AMPs) are defense molecules widely distributed and represent a promising alternative for solving the problem of antibiotic resistance. Nevertheless, the experimental time needed to screen putative AMPs makes computational simulations based on peptide sequence analysis and/or molecular modeling extremely attractive. Artificial intellig
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/07/2012
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5. Utilização de máquinas de suporte vetorial para predição de estruturas terciárias de proteínas / Support vector machine for tertiary structure prediction
The three-dimensional structure of a protein is directly related to its function. Many projects of genetic sequence analysis accumulate a great number of protein sequences whose primary and secondary structures are known. However, the information on its three-dimensional structures are available only for a small fraction of these proteins. This fact evidence
Publicado em: 2007
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6. Study of conformational changes of macromolecules in solutions using X-ray scattering / Estudo de mudanças conformacionais de macromoleculas em solução usando espalhamento de raio-X
During the last decades, the study of conformational changes in biological macromolecules has been a great challenge for the scientists, and continues to be an important subject of biotechnological interest and protein engineering. The process of folding (unfolding) of protein molecules has been intensively studied, because this investigation can contribute
Publicado em: 2007
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7. Evolutionary algorithms, to proteins structures prediction / Algoritmos evolutivos para predição de estruturas de proteínas
A Determinação da Estrutura tridimensional de Proteínas (DEP) a partir da sua seqüência de aminoácidos é importante para a engenharia de proteínas e o desenvolvimento de novos fármacos. Uma alternativa para este problema tem sido a aplicação de técnicas de computação evolutiva. As abordagens utilizando Algoritmos Evolutivos (AEs) tem obtido res
Publicado em: 2006
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8. The powerful high pressure tool for protein conformational studies
The pressure behavior of proteins may be summarized as a the pressure-induced disordering of their structures. This thermodynamic parameter has effects on proteins that are similar but not identical to those induced by temperature, the other thermodynamic parameter. Of particular importance are the intermolecular interactions that follow partial protein unfo
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-08
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9. The use of protein structure/activity relationships in the rational design of stable particulate delivery systems
The recombinant heat shock protein (18 kDa-hsp) from Mycobacterium leprae was studied as a T-epitope model for vaccine development. We present a structural analysis of the stability of recombinant 18 kDa-hsp during different processing steps. Circular dichroism and ELISA were used to monitor protein structure after thermal stress, lyophilization and chemical
Brazilian Journal of Medical and Biological Research. Publicado em: 2002-06
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10. Coupled prediction of protein secondary and tertiary structure
The strong coupling between secondary and tertiary structure formation in protein folding is neglected in most structure prediction methods. In this work we investigate the extent to which nonlocal interactions in predicted tertiary structures can be used to improve secondary structure prediction. The architecture of a neural network for secondary structure
National Academy of Sciences.
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11. Epitope mapping by mutagenesis distinguishes between the two tertiary structures of the histidine-containing protein HPr.
Thirty-four of the 85 residues of the histidine-containing protein HPr of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system have been changed by site-directed mutagenesis. Many of the mutations have wild-type activity suggesting an unaltered tertiary structure but have altered binding to three monoclonal antibodies: Jel42, Jel44, and J
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12. Annotation in three dimensions. PINTS: Patterns in Non-homologous Tertiary Structures
The detection of local structural patterns in proteins (e.g. active sites) can provide insights into protein function in the absence of sequence or fold similarity. Methods to detect such similarities are key during structural annotation, for example with results from Structural Genomics initiatives. PINTS (Patterns in Non-homologous Tertiary Structures, htt
Oxford University Press.