Tfam
Mostrando 1-12 de 21 artigos, teses e dissertações.
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1. Total flavonoids from Ampelopsis megalophylla suppress proliferation of vascular smooth muscle cells in vivo and in vitro
ABSTRACT Various benefits of flavonoids for ameliorating cardiovascular diseases have been demonstrated. However, the lowering effects on blood pressure caused by antiproliferative potentials of flavonoids in vascular smooth muscle cells are rare. In this study, the antihypertensive effects of total flavonoids from Ampelopsis megalophylla were investigated.
Braz. J. Pharm. Sci.. Publicado em: 01/02/2018
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2. Brain Region Specificity of Mitochondrial Biogenesis and Bioenergetics Response to Nrf2 Knockdown: A Comparison Among Hippocampus, Prefrontal Cortex and Amygdala of Male Rat Brain
ABSTRACT Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) has been identified as the well-known coordinator of intracellular antioxidant defense system. Herein, we aimed to evaluate the effects of Nrf2 silencing on mitochondrial biogenesis markers peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), nuclear respiratory factor-1(N
Braz. arch. biol. technol.. Publicado em: 17/08/2017
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3. eNOS se correlaciona com a biogênese mitocondrial em corações com cardiopatia congênita e cianose
FUNDAMENTO: O programa de biogênese mitocondrial no coração parece apresentar remodelação adaptativa após estresse biomecânico e oxidativo. Os mecanismos adaptativos que protegem o metabolismo do miocárdio durante a hipóxia são coordenados, em parte, pelo óxido nítrico (NO). OBJETIVO: Observar a biogênese mitocondrial e expressão do óxido nít
Arq. Bras. Cardiol.. Publicado em: 02/08/2012
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4. O papel da FAK (Focal Adhesion Kinase) na biogênese mitocondrial cardíaca induzida por estresse mecânico / A role for Focal Adhesion Kinase in cardiac mitochondrial biogenesis induced by mechanical stress
Estudamos o gene da FAK (focal adhesion kinase) na mitocondriogênese cardíaca induzida por estresse mecânico. O estresse prolongado (2-12 hs) de miócitos do ventrículo esquerdo de ratos neonatos(NRVM) aumentou a expressão do regulador transcricional mitocondrial PGC-1? (peroxisome proliferator activated receptor coactivator-1), NRF-1 (nuclear respirato
Publicado em: 2011
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5. Alterações metabólicas e o papel da mitocôndria no processo de tumorigênese de astrocitomas humanos / Metabolic alterations and the role of mitochondria in tumorigenic process of human astrocytomas
Mitochondria has a key role in cell survival and death. Mitochondrial DNA (mtDNA) alterations, for example, amplification, homoplasmic mutation, deletion and depletion, and their clinical and pathological implications have been analyzed in human malignancies. In order to search for mitochondrial alterations associated to tumorigenesis, this study aimed to an
Publicado em: 2010
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6. Architectural Role of Mitochondrial Transcription Factor A in Maintenance of Human Mitochondrial DNA
Mitochondrial transcription factor A (TFAM), a transcription factor for mitochondrial DNA (mtDNA) that also possesses the property of nonspecific DNA binding, is essential for maintenance of mtDNA. To clarify the role of TFAM, we repressed the expression of endogenous TFAM in HeLa cells by RNA interference. The amount of TFAM decreased maximally to about 15%
American Society for Microbiology.
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7. Human mitochondrial DNA is packaged with TFAM
Mitochondrial transcription factor A (TFAM), a member of the high mobility group proteins, is essential for maintenance of mitochondrial DNA (mtDNA). Most TFAM and mtDNA (both of which are normally soluble) was recovered from the particulate fraction of human placental mitochondria when extracted with the non-ionic detergent Nonidet P-40. mtDNA and TFAM were
Oxford University Press.
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8. Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells
Mitochondrial transcription factor A (TFAM) stimulates transcription from mitochondrial DNA (mtDNA) promoters in vitro and in organello. To investigate whether changes of TFAM levels also modulate transcription and replication in situ, the protein was transiently overexpressed in cultured cells. Mitochondrial mRNAs were significantly elevated at early time p
Oxford University Press.
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9. Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA
Mitochondrial transcription factor A (TFAM) has been shown to stimulate transcription from mitochondrial DNA promoters in vitro. In order to determine whether changes in TFAM levels also regulate RNA synthesis in situ, recombinant human precursor proteins were imported into the matrix of rat liver mitochondria. After uptake of wt-TFAM, incorporation of [α-3
Oxford University Press.
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10. Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein
During replication, mitochondrial DNA (mtDNA) takes on a triple-stranded structure called a D-loop. Although their physiological roles are not understood, D-loops are implicated in replication and transcription of mtDNA. Little is known about the turnover of D-loops. We investigated the effects of mitochondrial transcription factor A (TFAM) and single-strand
Oxford University Press.
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11. The mitochondrial RNA polymerase contributes critically to promoter specificity in mammalian cells
Initiation of transcription in mammalian mitochondria depends on three proteins: mitochondrial RNA polymerase (POLRMT), mitochondrial transcription factor A (TFAM) and mitochondrial transcription factor B2 (TFB2M). We show here that the recombinant mouse and human transcription machineries are unable to initiate transcription in vitro from the heterologous l
Nature Publishing Group.
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12. Increased in vivo apoptosis in cells lacking mitochondrial DNA gene expression
We have attempted to determine whether loss of mtDNA and respiratory chain function result in apoptosis in vivo. Apoptosis was studied in embryos with homozygous disruption of the mitochondrial transcription factor A gene (Tfam) and tissue-specific Tfam knockout animals with severe respiratory chain deficiency in the heart. We found massive apoptosis i
The National Academy of Sciences.