Thin Layer Cultivation
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Associação da técnica de cultivo em camada delgada contendo ágar Middlebrook 7H11 Modificado com a Reação em Cadeia de Polimerase (PCR) para identificação precoce de Mycobacterium bovis em órgãos de bovinos e bubalinos oriundos de abatedouros comerciais / Association between the modified Middlebrook 7H11 agar thin layer cultivation technique with the Polymerase Chain Reaction (PCR) on the earlier identification of Mycobacterium bovis in bovine and buffalo organs deriving from commercial slaughterhouses
The modified thin layer Middlebrook 7H11 cultivation was compared to the standard culture technique containing Stonebrink media, in order to evaluate the sensitivity and time for the detection of Mycobacterium bovis in bovine and buffalo organs deriving from commercial slaughterhouses. The PCR was applied for confirmation of the growth observed in the cultur
Publicado em: 2010
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2. Avaliação da atividade micobactericida de desinfetantes químicos utilizando a técnica de cultivo em camada de ágar Middlebrook 7H11 / Evaluation of mycobactericidal activity of chemical disinfectants using the Middlebrook 7H11 agar medium technique
The mycobactericidal activity of five chemical disinfectants was evaluated against a strain of Mycobacterium bovis isolated from a goat, typified by PCR (polymerase chain reaction) and with 32 days of growth in the Stonebrink medium. The disinfectants were tested using the modified thin layer Middlebrook 7H11 cultivation technique and it was compared to the
Publicado em: 2007
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3. A Tetrodotoxin-Producing Vibrio Strain, LM-1, from the Puffer Fish Fugu vermicularis radiatus
Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated th
American Society for Microbiology.
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4. Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L. 1
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities o
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5. Detection of microbial proteolytic activity by a cultivation plate assay in which different proteins adsorbed to a hydrophobic surface are used as substrates.
A screening technique for microbial proteases, the thin-layer enzyme assay cultivation technique, was developed. The inner surface of a polystyrene petri dish was coated with protein and then covered with a culture agar medium. The enzymes, produced during growth of the microorganisms, reach the protein-coated surface by diffusion in the agar. Degradation of
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6. Fibrinogenolytic and fibrinolytic activity in oral microorganisms.
Samples were taken from blood accumulated in dental alveoli after surgical removal of mandibular third molars, from subgingival plaque of teeth with advanced periodontal destructions, from teeth with infected necrotic pulps, and from subjects suffering from angular cheilitis. Of the microorganisms subcultured from these samples, 116 strains were assayed for
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7. Microbiological Transformations of Δ6a,10a-Tetrahydrocannabinol
A screening program was conducted to find microorganisms that catalyze transformation reactions with cannabinoids. Three hundred fifty-eight cultures, consisting of 97 bacteria, 175 actinomycetes, and 86 molds, were incubated in media containing 0.5 mg of Δ6a,10a-tetrahydrocannabinol (Δ6a,10a-THC) per ml. After 120 h of cultivation, ethyl acetate extracts
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8. Degradation of human immunoglobulins by proteases from Streptococcus pneumoniae obtained from various human sources.
The ability of Streptococcus pneumoniae to degrade human secretory immunoglobulin A (S-IgA), IgG, and IgM was tested in 102 strains by use of the thin-layer enzyme assay cultivation technique. The strains were isolated from patients with acute phases of otitis media, meningitis, and pneumonia as well as from symptomless carriers. An ability to degrade S-IgA,
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9. In vitro cultivation of human islets from expanded ductal tissue
A major obstacle to successful islet transplantation for both type 1 and 2 diabetes is an inadequate supply of insulin-producing tissue. This need for transplantable human islets has stimulated efforts to expand existing pancreatic islets and/or grow new ones. To test the hypothesis that human adult duct tissue could be expanded and differentiated in vitro t
The National Academy of Sciences.
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10. Role of endogenous prostaglandin E2 in erythropoietin production and dome formation by human renal carcinoma cells in culture.
Studies were carried out on the role of endogenous prostaglandin E2 (PGE2) in erythropoietin (Ep) production and dome formation in primary monolayer cultures of a human renal carcinoma from a patient with erythrocytosis that has been serially transplanted into BALB/c athymic nude mice. The metabolism of [14C]arachidonic acid (14C-AA) by cultured renal carcin