Thioguanine
Mostrando 1-12 de 105 artigos, teses e dissertações.
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1. Improving the outcomes of elderly patients with acute myeloid leukemia in a Brazilian University Hospital
OBJECTIVE: To evaluate the outcomes of acute myeloid leukemia patients who were older than 60 years of age at the time of diagnosis following the implementation of a treatment algorithm based on age, performance status, and cytogenetic results. METHODS: We retrospectively compared the results of 31 elderly acute myeloid leukemia patients (median age of 74 ye
Clinics. Publicado em: 2011
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2. Desenvolvimento de metodologia para monitorização terapêutica da azatioprina por cromatografia líquida de alta eficiência-UV (HPLC-UV) em transplantados renais / Development of a methodology for therapeutic drug monitoring of azathioprine by high performance liquid chromatography-UV (HPLC-UV) in renal transplant recipients
Azathioprine (AZA) is an immunosuppressant used in autoimmune pathologies like lupus erythematosus, Chrons disease, inflammatory bowel disease and against rejection in solid organs transplant. After more than 40 years of use, AZA continues exerting a central role in immunomodulatory regimens, due to the fact that it combines effectiveness, safety and low cos
Publicado em: 2010
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3. Simultaneous determination of captopril and thioguanine in pharmaceutical compounds and blood using cathodic adsorptive stripping voltammetry
Apresentamos um procedimento altamente seletivo e sensível para a determinação simultânea de captopril e de tioguanina baseado em processo de redissolução catódica adsortiva dos complexos de Cu(I)-captopril e de Cu(I)-tioguanina em um eletrodo gotejante de mercúrio. Captopril e tioguanina foram pré-concentrados na superfície da gota do eletrodo de
Journal of the Brazilian Chemical Society. Publicado em: 2008
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4. An improved HPLC method for the quantitation of 6-mercaptopurine and its metabolites in red blood cells
A procedure is described for the rapid determination of the intra-erythrocyte concentration of 6-mercaptopurine (6-MP) and its metabolites, 6-thioguanine nucleotides (6-TGN) and 6-methylmercaptopurine (6-MMP). Erythrocytes (8 x 10(8) cells) in 350 µl Hanks solution containing 7.5 mg dithiothreitol were treated with 50 µl 70% perchloric acid. The precipitat
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-05
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5. Effect of 6-thioguanine on Chlamydia trachomatis growth in wild-type and hypoxanthine-guanine phosphoribosyltransferase-deficient cells.
Chlamydiae have evolved a biphasic life cycle to facilitate their survival in two discontinuous habitats. The unique growth cycle is represented by two alternating forms of the organism, the elementary body and the reticulate body. Chlamydiae have an absolute nutritional dependency on the host cell to provide ribonucleoside triphosphates and other essential
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6. T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood.
Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistance had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in
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7. Guanine analog-induced differentiation of human promyelocytic leukemia cells and changes in queuine modification of tRNA.
Treatment of hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-deficient human promyelocytic leukemia (HL-60) cells with 6-thioguanine results in growth inhibition and cell differentiation. 6-Thioguanine is a substrate for the tRNA modification enzyme tRNA-guanine ribosyltransferase, which normally catalyzes the exchange of queuine for guanine in positi
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8. Microbiological Assay and Tissue Distribution of β-Thioguanine Deoxyriboside in Mice 1
A sensitive, precise microbiological assay has been developed for the determination of tissue distribution of β-thioguanine deoxyriboside, a new antitumor agent.
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9. Induction of Thioguanine- and Ouabain-Resistant Mutants and Single-Strand Breaks in the DNA of Chinese Hamster Ovary Cells by 3H-Thymidine
Cultured Chinese hamster cells were labeled with 6-3H-thymidine or 5-methyl-3H-thymidine and allowed to accumulate damage from 3H decays for various periods of time while frozen. The frequencies of cells resistant to 6-thioguanine or ouabain and the amount of DNA damage (i.e., number of single-strand breaks) were determined and compared with the mutation fr
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10. Mutagenesis in S49 mouse lymphoma cells: induction of resistance to ouabain, 6-thioguanine, and dibutyryl cyclic AMP.
The effects of mutagens on three genetic markers--resistance to ouabain, 6-thioguanine, and dibutyryl cyclic AMP (Bt2cAMP), were investigated in a mouse lymphoma cell line, S49. Nitrosoguanidine, ethyl methanesulfonate, ICR 191, and x-rays were used. Mutagen-specific responses were seen. Ouabain resistance was induced by nitrosoguanidine, but not by ICR 191.
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11. Use of Escherichia coli Mutants to Evaluate Purines, Purine Nucleosides, and Analogues
Of 142 purines, purine nucleosides, and analogues tested for inhibition of growth of Escherichia coli B Hill, 45 were active. Of these, 27 were evaluated for inhibition of other E. coli lines, including those resistant to 6-thioguanine, 2-fluoroadenosine, 2,6-diaminopurine, or 6-mercaptopurine. Most toxic to the parent lines were 2-fluoroadenosine, 2-fluoroa
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12. 6-Thioguanine as a cause of toxic veno-occlusive disease of the liver
Lesions of hepatic veno-occlusive disease were found in the needle biopsy specimen of one patient suffering from chronic granulocytic leukaemia and in the liver at necropsy of a second patient suffering from acute myeloid leukaemia. The treatment included administration of 6-thioguanine which was the only relevant compound used in the first patient and which