Three Dimensional Cell Culture
Mostrando 1-12 de 92 artigos, teses e dissertações.
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1. Three-dimensional cell culture for the study of nasal polyps
Abstract Objectives: Three-dimensional (3D) cell cultures have many applications such as stem cell biology research, new drug discovery, cancer, and Chronic Rhinosinusitis with Nasal Polyps (CRSwNP). This disease is characterized by a significant impact on quality of life and productivity. The diversity of factors that act in the progression of CRSwNP point
Brazilian Journal of Otorhinolaryngology. Publicado em: 2022
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2. Expansion of cord blood stem cells in fibronectin-coated microfluidic bioreactor
ABSTRACT Background: Hematopoietic stem/progenitor cell transplantation is the main treatment option for hematological malignancies and disorders. One strategy to solve the problem of low stem cell doses used in transplantation is pre-transplant expansion. We hypothesized that using fibronectin-coated microfluidic channels would expand HSPCs and keep self-r
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
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3. Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion
Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on h
Braz J Med Biol Res. Publicado em: 26/03/2018
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4. Response of a co-culture model of epithelial cells and gingival fibroblasts to zoledronic acid
Abstract Osteonecrosis of the jaw is an adverse effect of bisphosphonates. While the etiopathogenesis of this condition has been investigated, the interactions and effects of bisphosphonates on oral mucosa cells remain unclear. It is hypothesized that cell culture models, such as co-culture or three-dimensional cell culture models, can provide valuable insig
Braz. oral res.. Publicado em: 28/11/2016
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5. Expression of osteoblastic phenotype in periodontal ligament fibroblasts cultured in three-dimensional collagen gel
Objective : To investigate the influence of a three-dimensional cell culture model on the expression of osteoblastic phenotype in human periodontal ligament fibroblast (hPDLF) cultures. Material and Methods : hPDLF were seeded on bi-dimensional (2D) and three-dimensional (3D) collagen type I (experimental groups) and and on a plastic coverslip (control) for
J. Appl. Oral Sci.. Publicado em: 2015-04
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6. A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay
Current therapy for pancreatic cancer is multimodal, involving surgery and chemotherapy. However, development of pancreatic cancer therapies requires a thorough evaluation of drug efficacy in vitro before animal testing and subsequent clinical trials. Compared to two-dimensional culture of cell monolayer, three-dimensional (3-D) models more closely mimic nat
Braz J Med Biol Res. Publicado em: 22/07/2013
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7. Expressão dos fenótipos fibroblástico e osteoblástico em culturas tridimensionais na presença de partículas de vidro bioativo / Osteoblastic and fibroblastic phenotypes expression on three dimensional cell cultures in the presence of bioactive glass particles
O objetivo deste estudo foi analisar a expressão dos fenótipos fibroblástico e osteoblástico em culturas tridimensionais na presença ou não de partículas de vidro bioativo. Fibroblastos derivados do ligamento periodontal humano (hPDLF) e células osteogênicas da calvária de rato recém-nascidos foram plaqueadas em superfícies bidimensionais - lamí
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/10/2012
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8. Estudo dos efeitos da terapia fotodinâmica na progressão tumoral e em modelos celulares tridimensionais / Study of the effects of photodynamic therapy on tumor progression and in three-dimensional cellular models.
Melanoma is the type of skin cancer with poor prognosis due to its high probability of suffering metastases. Currently there are few effective treatments for the this, and the survival of patients with metastases is very low, around 8 months, with cure rates of these conditions do not exceed 1%. Photodynamic therapy (PDT) is a therapeutic modality selective
Publicado em: 2010
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9. Avaliação in vitro de matriz dérmica acelular como arcabouço tridimensional para cultivo de fibroblastos gengivais / In vitro evaluation of acellular dermal matrix as a three-dimensional scaffold for gingival fibroblasts seeding
Fibroblastos gengivais desempenham um importante papel na regeneração de tecidos moles de proteção do periodonto. Alloderm® (MDA) é um substituto alógeno muito utilizado e estudado em periodontia. O objetivo do presente estudo foi avaliar, in vitro, se a MDA é uma matriz tridimensional adequada, através de sua resposta à cultura de fibroblastos gen
Publicado em: 2010
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10. Avaliação da eficácia e segurança do extrato de folhas de Rubus rosaefolius Sm. visando a aplicação como conservante em produtos cosméticos / Effectiveness and safety evaluation of Rubus rosaefolius Sm. leaves, regarding to the preservative use in cosmetic products
Cosmetic and pharmaceuticals products contained natural compounds have increased in the last few years. To verify the use of these compounds as preservative in formulations, the antimicrobial activity from the raw extract of the Rubus rosaefolius Sm. leaves and its fractions was determined. The toxicity in vitro and the behavior in cosmetic formulations (gel
Publicado em: 2009
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11. Alterações na expressão protéica das células mesangiais em cultura sob os efeitos quimiotóxicos do meio de contraste radiológico iodado, HexabrixR. / Juliana Dinéia Perez. - São Paulo, 2006. / Effects of radiological contrast medium, HexabrixTM in mesangial cell protein expression in culture.
The kidney is a common target organ for toxic agents, like contrast media (CM). The CM with iodine is normally used in angiography, urography and tomography. CM is filtrated and metabolized in the kidney, being the major cause of acute renal failure (ARF). Nowadays, proteome is actually a powerful tool in the Nephrology Research, mainly in the identification
Publicado em: 2006
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12. Expressão de PRSS11, MTSS1, CLPTM1, SMYD2, NOTCH1 e RPL37A em fatias de carcinoma de mama de cadelas e correlação com resposta à doxorrubicina in vitro / Expression of PRSS11, MTSS1, CLPTM1, SMYD2, NOTCH1 and RPL37A in slices of bitch mammary cancer and correlation with response to exposure doxorubicin in vitro
Mammary gland tumors are the most common neoplasias of bitches and doxorubicin is one the mainstay drugs used in their treatment. Neoadjuvant chemotherapy with doxorubicin not only reduces the primary tumor as well as allows an in vivo evaluation of tumor response. Predictive markers of response, if identified, might allow a tailored treatment of each patien
Publicado em: 2006