Thymine
Mostrando 1-12 de 1045 artigos, teses e dissertações.
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1. A Novel Electrochemical Aptamer Biosensor Based on DNAzyme Decorated Au@Ag Core-Shell Nanoparticles for Hg2+ Determination
As a kind of important water pollutant, heavy metal ions have a detrimental effect on the health of human. In this study, Au@Ag core-shell nanoparticles were synthesized by simple reduction of Ag + around Au nanoparticles and functionalized by DNAzyme. Based on thymine-Hg2+-thymine binding mode, a sensitive aptamer biosensor was constructed by utilizing func
J. Braz. Chem. Soc.. Publicado em: 2018-02
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2. Protease gene shuffling and expression in Pichia pastoris
Four kinds of neutral and alkaline protease genes from Aspergillus oryzae and Bacillus subtilis were isolated and shuffled. The shuffled genes were selected, inserted into pGAPZαA plasmid and transformed into Escherichia coli. The gene which could express high-activity protease was selected by screening the sizes of transparent zones around the colonies on
Braz. arch. biol. technol.. Publicado em: 2015-06
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3. DNA methylation patterns of candidate genes regulated by thymine DNA glycosylase in patients with TP53 germline mutations
Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant, hereditary cancer predisposition disorder. In Brazil, the p.R337H TP53 founder mutation causes the variant form of LFS, Li-Fraumeni-like syndrome. The occurrence of cancer and age of disease onset are known to vary, even in patients carrying the same mutation, and several mechanisms such as genetic an
Braz J Med Biol Res. Publicado em: 28/04/2015
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4. Hepatitis B virus genotypes and mutations in the basal core promoter and pre-core/core in chronically infected patients in southern Brazil: a cross-sectional study of HBV genotypes and mutations in chronic carriers
Introduction In Brazil, little data exist regarding the distribution of genotypes in relation to basal core promoter (BCP) and precore/core mutations among chronic hepatitis B virus (HBV) carriers from different regions of the country. The aim of
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-12
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5. SNPs genotyping technologies and their applications in farm animals breeding programs: review
Single nucleotide polymorphisms (SNPs) are DNA sequence variations that occur when a single nucleotide: adenine (A), thymine (T), cytosine (C) or guanine (G) in the genome sequence is altered. Traditional and high throughput methods are two main strategies for SNPs genotyping. The SNPs genotyping technologies provide powerful resources for animal breeding pr
Braz. arch. biol. technol.. Publicado em: 2014-02
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6. 99mTc-thymine scintigraphy may be a promising method in the diagnosis of breast cancer
OBJECTIVE: Mammography has been established as the gold standard for the detection of breast cancer, and imaging techniques such as ultrasonography, magnetic resonance imaging, scintigraphy and positron emission tomography may be useful to improve its sensitivity and specificity. The objective of this study with breast scintigraphy was to evaluate the uptake
Clinics. Publicado em: 2013
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7. Investigações de interações hiperfinas de DNA e anticorpos de diferentes linhagens de camundongos frente à infecção por T. cruzi pela espectroscopia de correlação angular gama-gama perturbada / Investigation of hyperfine interactions in DNA and antibody of different lineages of mice infected by T. cruzi by perturbed gamma-gamma angular correlation spectroscopy
In the present work perturbed angular correlation (PAC) spectroscopy was used to measured electric quadrupole interactions in DNA biomolecules of different mice lineages (A/J, C57BL/6, B6AF1, BXA1 e BXA2), samples of different isotypes of immunoglobulin G (IgG1, IgG2a e IgG2b) and active portions of complete and fragmented immunoglobulin responsible by the i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/02/2012
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8. Association of the 894G>T polymorphism of the endothelial constitutive nitric oxide synthase gene with unstable angina
The 894G>T polymorphism of the endothelial constitutive nitric oxide synthase gene consists of the substitution of a guanine base by a thymine at the 894th nucleotide of the gene. An association of this polymorphism with acute coronary syndromes has been described, only when in combination with other polymorphisms of this gene. The aim of the present study w
Publicado em: 2010
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9. Avaliação do perfil metabólico da estavudina através do emprego da bioconversão e da modelagem molecular do citocromo P-450 CYP3A4 / Evaluation of the metabolic profile of stavudine through the use of bioconversion and molecular modeling of cytochrome P-450 CYP3A4
Before the approval of an active compound, metabolism studies are necessary to ensure its safety, once active metabolites could be synthesized during human biotransformation. The use of eukaryotic microorganisms for the study of drug metabolism has been widely explored, due to its capability of producing metabolites similar to the mammalians, and in silico s
Publicado em: 2009
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10. Frequency of UGT1A1*28 (Gibert´s syndrome) in patients with chronic hepatitis C virus and healthy donors / Freqüência do alelo UGT1A1*28 (síndrome de Gilbert) em pacientes portadores de hepatite crônica C e em controles sadios
Gilberts syndrome is a benign condition characterized by unconjugated hiperbilurubinemia that occurs in the absence of hemolysis or liver chronic disease. It is clinically manifested by intermittently jaundice, triggered by exposition to physical stress, low calory diet, among others. The genetic base is the reduction of the activity of UDP-glucuronosyltrans
Publicado em: 2009
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11. CaracterizaÃÃo genÃtica do vÃrus da hepatite B em Alagoas
Hepatitis B virus (HBV) has been classified into eight genotypes (A to H), with distinct geographical distributions. The divergences between the genotypes have been defined arbitrarily, based on a difference of more than 8% in the virus genome sequence. Naturally variants are found in regions pre-S/S, pre-core/C and P. The mutation of the precore region (G18
Publicado em: 2009
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12. Desenvolvimento e validação de método por cromatografia líquida de alta eficiência para determinação simultânea das impurezas timina e timidina na matéria-prima estavudina
A HPLC method was developed to quantify thymine and thymidine impurities in stavudine bulk drug. The separation was carried out in isocratic mode using methanol/water (20:80) as mobile phase, a C18 column and UV detection at 266 nm. The method provided selectivity based on peak purities and resolution among peaks. It was linear over the range of 0.5-5.0 µg/
Química Nova. Publicado em: 2008