Tissue Culture In Vitro
Mostrando 1-12 de 980 artigos, teses e dissertações.
-
1. Evaluation of different protocols for culturing mesenchymal stem cells derived from murine bone marrow
ABSTRACT Introduction: Culturing bone marrow mesenchymal stem cells (BM-MSCs) is a key point in different fields of research, including tissue engineering and regenerative medicine and studies of the bone marrow microenvironment. However, isolating and expanding murine BM-MSCs in vitro has challenged researchers due to the low purity and yield of obtained c
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
-
2. Ovarian Tissue Culture to Preserve Fertility in Transgender Male Patients after Hormonal Treatment
Resumo Objetivo Avaliar as características reprodutivas e histológicas de tecido ovariano cultivado a fresco de pacientes transexuais masculinos. Métodos Estudo experimental in vitro e piloto, no qual amostras foram coletadas durante a cirurgia de redesignação de sexo para pacientes transexuais masculinos. O córtex ovariano foi cortado em fragmento
Revista Brasileira de Ginecologia e Obstetrícia. Publicado em: 2022
-
3. Inhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide
ABSTRACT Zika virus (ZIKV) infection during pregnancy is associated with a congenital syndrome. Although the virus can be detected in human placental tissue and sexual transmission has been verified, it is not clear how the virus reaches the fetus. Despite the emerging severity caused by ZIKV infection, no specific prophylactic and/or therapeutic treatment i
Braz J Infect Dis. Publicado em: 2020-12
-
4. Standardization of organoid culture for evaluation of melanogenesis induced by UVB, UVA and visible light,
Abstract Background: Organoid cultures are primary cultures that maintain architectural characteristics and the relationships between cells, as well as the extracellular matrix. They are alternatives for pathophysiological or therapeutic investigation rather than animal and in vitro tests. Objective: Development of a cutaneous organoid culture model, aimin
An. Bras. Dermatol.. Publicado em: 2020-02
-
5. Generation of Yellow Fever virus vaccine in skeletal muscle cells of chicken embryos
BACKGROUND The Yellow Fever (YF) vaccine is produced by the inoculation of embryonated chicken eggs with YF17DD virus on the ninth day of development. Full embryos are collected on the twelfth day of development for vaccine formulation. Skeletal muscle tissue is the main site where biosynthesis of viral particles occurs. OBJECTIVES This study aimed to an
Mem. Inst. Oswaldo Cruz. Publicado em: 09/12/2019
-
6. Powdered coconut water (ACP 406®) as an alternative base culture medium for in vitro culture of goat preantral follicles enclosed in ovarian tissue
Abstract This study evaluated a powdered coconut water solution (ACP 406®) as a base culture medium on the in vitro survival and development of in situ goat preantral follicles. The ovarian fragments were either immediately fixed in Carnoy solution (non-cultured control) or individually cultured for 2 or 6 days. The following culture media (all containing 1
Anim. Reprod.. Publicado em: 25/11/2019
-
7. Feasibility of the new method for orchid in vitro rooting using liquid and chemical sterilized culture medium under different sucrose concentration
Resumo A propagação in vitro de orquídeas é a única técnica comercial em larga escala para obtenção de plântulas saudáveis e de alta qualidade com origem clonal. O uso de novas tecnologias em sistemas de cultura de tecidos vegetais pode levar a aumentos de eficiência e redução de custos dos sistemas de micropropagação. O principal sistema de m
Ornam. Hortic.. Publicado em: 28/10/2019
-
8. Role of Candida albicans on enamel demineralization and on acidogenic potential of Streptococcus mutans in vitro biofilms
Abstract There is growing evidence that C. albicans is associated with dental caries, but its role on caries development needs to be better clarified. Objective: To evaluate at the hard tissue level the effect of C. albicans on the cariogenic potential of S. mutans biofilms focusing on the mineral profile of induced carious lesions. This study also aimed to
J. Appl. Oral Sci.. Publicado em: 09/09/2019
-
9. Isolation and characterization of a human cementocyte-like cell line, HCY-23
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we investigate whether cementocytes, the residing cells in ce
Braz. oral res.. Publicado em: 15/08/2019
-
10. Advances and challenges on the in vitro production of secondary metabolites from medicinal plants
RESUMO A produção de metabólitos secundários de plantas medicinais, também chamados de Compostos Medicinais Derivados de Plantas (PDMC), vem ganhando importância na última década. Concomitante ao aumento do conhecimento sobre as propriedades farmacológicas destes compostos, as plantas da horticultura tornam-se a fonte de biomassa mais importante, su
Hortic. Bras.. Publicado em: 18/07/2019
-
11. In vitro propagation of alpinia cultivars in different light sources
Resumo A fonte de luz na cultura de tecidos muitas vezes é um dos fatores pontuais para a adequação da produção de plantas, para isso, vêm se utilizando os diodos emissores de luz (LEDs) no intuito de aumentar o crescimento e desenvolvimento das plantas propagadas in vitro. O objetivo do trabalho foi avaliar a propagação in vitro de cultivares de Alp
Ornam. Hortic.. Publicado em: 29/04/2019
-
12. Standardization and quality assessment for clinical grade mesenchymal stem cells from human adipose tissue
Abstract Background Mesenchymal stem cells have immense potential in stem cell-based therapies, however there is a pre-requisite to develop a curative cell dose. Adipose tissue-derived mesenchymal stem cells are promising mainly due to their potential abundance, immunomodulatory effect and remarkable differentiation potential. Nevertheless, senescence may d
Hematol., Transfus. Cell Ther.. Publicado em: 15/04/2019