Two Dimensional Gel
Mostrando 1-12 de 1507 artigos, teses e dissertações.
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1. Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)
ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological
Rev. Bras. entomol.. Publicado em: 13/01/2020
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2. CARACTERIZAÇÃO QUÍMICA E AVALIAÇÃO DA CITOTOXICIDADE DE UM HETEROPOLISSACARÍDEO ISOLADO DA BIOMASSA DO Colletotrichum gloeosporioides
Colletotrichum gloeosporioides biomass was submitted to ethanolic extraction (78 ºC, 12 h) to remove low molecular weight compounds followed by hot aqueous extraction (100 ºC, 4 h, 4x) to obtain polysaccharides. Consecutive freezing/thawing treatments of the aqueous extract yielded a soluble fraction in cold water that was separated on five components (PI
Quím. Nova. Publicado em: 27/05/2019
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3. Hyaluronic acid accelerates bone repair in human dental sockets: a randomized triple-blind clinical trial
Abstract This study evaluated the effects of hyaluronic acid (HA) on bone repair of human dental sockets. Thirty-two lower first premolars were extracted from 16 patients (2 per patient) for orthodontic reasons. Following the extractions, one socket was randomly filled with 1% HA gel, while the other was allowed to naturally fill with blood clot. After 30 an
Braz. oral res.. Publicado em: 13/09/2018
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4. Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats wit
Braz J Med Biol Res. Publicado em: 11/01/2018
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5. Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid
Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and ident
Braz J Med Biol Res. Publicado em: 03/07/2017
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6. Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Abstract INTRODUCTION: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. METHODS: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacryla
Rev. Soc. Bras. Med. Trop.. Publicado em: 2017-06
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7. Synthesis, characterization and cytotoxicity of Chitosan/Polyvinyl Alcohol/Bioactive Glass hybrid scaffolds obtained by lyophilization
ABSTRACT One of the important research topics in tissue engineering is the development of optimum three-dimensional scaffolds for regeneration and growth of bone tissue. The scaffold developed should promote an initial biomechanical support, provide the formation, deposition and organization of the new organic matrix generated, and degrade proportionally to
Matéria (Rio J.). Publicado em: 2016-12
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8. Screening of the in vitro antileishmanial activities of compounds and secondary metabolites isolated from Maytenus guianensis Klotzsch ex Reissek (Celastraceae) chichuá Amazon
Abstract INTRODUCTION Maytenus guianensis is a member of the Celastraceae family that is used in traditional medicine, particularly for its anti-parasitic and anti-cancer effects. To explore the ethnopharmacological potential of this plant, the present study was designed to screen the in vitro antileishmanial activities of extracts and compounds isolated fr
Rev. Soc. Bras. Med. Trop.. Publicado em: 2016-10
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9. Effects of (-)-6,6'-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cube
Rev. bras. farmacogn.. Publicado em: 2016-06
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10. Proteomic analysis of halotolerant proteins under high and low salt stress in Dunaliella salina using two-dimensional differential in-gel electrophoresis
Abstract Dunaliella salina, a single-celled marine alga with extreme salt tolerance, is an important model organism for studying fundamental extremophile survival mechanisms and their potential practical applications. In this study, two-dimensional differential in-gel electrophoresis (2D-DIGE) was used to investigate the expression of halotolerant proteins u
Genet. Mol. Biol.. Publicado em: 13/05/2016
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11. Candida albicans PROTEIN PROFILE CHANGES IN RESPONSE TO THE BUTANOLIC EXTRACT OF Sapindus saponaria L.
Candida albicans is an opportunistic human pathogen that is capable of causing superficial and systemic infections in immunocompromised patients. Extracts of Sapindus saponaria have been used as antimicrobial agents against various organisms. In the present study, we used a combination of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matri
Rev. Inst. Med. trop. S. Paulo. Publicado em: 08/04/2016
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12. Biological characterization of compounds from Rhinella schneideri poison that act on the complement system
Background The skin secretions of toads of the family Bufonidae contain biogenic amines, alkaloids, steroids (bufotoxins), bufodienolides (bufogenin), peptides and proteins. The poison of Rhinella schneideri, formerly classified as Bufo paracnemis, presents components that act on different biological systems, including the complement system. The aim of this
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 29/09/2015