Two Dimensional Immunoblotting
Mostrando 1-12 de 85 artigos, teses e dissertações.
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1. Identification of IgE and IgG1 specific antigens in Echinococcus granulosus cyst fluid
Cystic echinococcosis (CE) is an anthropozoonotic disease with worldwide distribution and is caused by the cestode Echinococcus granulosus. Anaphylactic shock induced by CE rupture is a serious complication especially in patients with hydatid infections, as the resulting leakage of fluid contains highly toxic endogenous antigen. We aimed to isolate and ident
Braz J Med Biol Res. Publicado em: 03/07/2017
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2. Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder
Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to iden
Braz J Med Biol Res. Publicado em: 08/09/2015
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3. 2D-immunoblotting analysis of Sporothrix schenckii cell wall
We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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4. Identificação de isoformas antigênicas de Dermatophagoides pteronyssinus reconhecidas por anticorpos IgE e IgG1 em pacientes atópicos e não atópicos
The house dust mite Dermatophagoides pteronyssinus (Dp) is a major source of indoor allergens in the world and considered important for sensitization of genetically predisposed subjects. Some allergens from this mite can occur as isoallergens with differences in amino acid sequences or glycosylation, leading to differences in allergenic sensitization. The ai
Publicado em: 2010
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5. Análise comparativa de mapas protéicos de amostras de soja convencionais e tolerantes ao herbicida glifosato visando à inocuidade alimentar / Comparative analysis of maps soy protein samples of conventional and tolerant to the herbicide glyphosate for food safety
A soja geneticamente modificada tolerante ao herbicida glifosato tem sido a cultura derivada da engenharia genética mais cultivada atualmente no mundo. Como todo alimento GM a soja tem sido alvo de investigação em relação a sua Biossegurança. Novas estratégias têm sido desenvolvidas e aplicadas neste campo de pesquisa, sendo que métodos rápidos e e
Publicado em: 2009
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6. Proteomic inventory of myocardial proteins from patients with chronic Chagas' cardiomyopathy
Chronic Chagas' disease cardiomyopathy (CCC) is an often fatal outcome of Trypanosoma cruzi infection, with a poorer prognosis than other cardiomyopathies. CCC is refractory to heart failure treatments, and is the major indication of heart transplantation in Latin America. A diffuse myocarditis, plus intense myocardial hypertrophy, damage and fibrosis, in th
Brazilian Journal of Medical and Biological Research. Publicado em: 30/10/2006
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7. Antigens of Brucella abortus S19 immunodominant for bovine lymphocytes as identified by one- and two-dimensional cellular immunoblotting.
Cellular immune responses are influential for protection against intracellular bacteria such as brucellae. Therefore, identification of Brucella abortus antigens that activate primed bovine lymphocytes is fundamental for discerning the breadth of cellular response in bovine brucellosis. Potentially antigenic components of B. abortus S19 were isolated by sodi
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8. Comparison of four Mycoplasma arthritidis strains by enzyme immunoassay, metabolism inhibition, one- and two-dimensional electrophoresis, and immunoblotting.
Four Mycoplasma arthritidis strains, two virulent (158p10p9 and 14124p10) and two avirulent (PG6 and H606), were examined for differences in their antigenic compositions. Rabbit antisera prepared against each strain were compared for their reactivities against both homologous and heterologous strains by enzyme-linked immunosorbent assay and metabolism inhibi
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9. Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins.
We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flagellar band with a molecular mass of 63 kil
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10. Sodium dodecyl sulfate- and salt-extracted antigens from various Brucella species induce proliferation of bovine lymphocytes.
One- and two-dimensional gel electrophoresis and cellular immunoblotting were used to compare the protein profiles and immunogenic capabilities of salt- and sodium dodecyl sulfate-extracted components isolated from different Brucella species. Cellular immunoblotting demonstrated that freshly isolated bovine peripheral blood mononuclear cells proliferated to
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11. The SWISS-2DPAGE database of two-dimensional polyacrylamide gel electrophoresis.
SWISS-2DPAGE is a database of proteins identified on two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), created and maintained at the University Hospital of Geneva in collaboration with the Department of Medical Biochemistry of Geneva University. The proteins have been identified on various 2-D PAGE reference maps by microsequencing, immunoblotti
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12. Identification of antigens recognized by T cells in human leishmaniasis: analysis of T-cell clones by immunoblotting.
Immunity in human leishmaniasis is mediated by sensitized T lymphocytes; however, the antigens involved in eliciting this immunity have not been defined. We describe the generation of human T-lymphocyte clones derived from two patients with healed leishmaniasis. By use of one- and two-dimensional cellular immunoblotting techniques, we directly identified the