Two Photon Excited Fluorescence
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. [RETRACTED ARTICLE] Characteristics of liver fibrosis with different etiologies using a fully quantitative fibrosis assessment tool
This study aimed to test the diagnostic performance of a fully quantitative fibrosis assessment tool for liver fibrosis in patients with chronic hepatitis B (CHB), primary biliary cirrhosis (PBC) and non-alcoholic steatohepatitis (NASH). A total of 117 patients with liver fibrosis were included in this study, including 50 patients with CHB, 49 patients with
Braz J Med Biol Res. Publicado em: 18/05/2017
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2. Microscopias de óptica não linear : fluorescência excitada por absorção de dois fótons, geração de segundo harmônico e geração de terceiro harmônico / Non linear optical microscopies : two photon excited fluorescence, second harmonic generation and third harmonic generation
Biologia celular é um novo mundo promissor com enorme impacto social, econômico e na saúde. Organismos vivos são capazes de, produzir a própria energia a partir da luz do sol, se reproduzir, de se auto-reparar, sinalizar e navegar por sinais bioquímicos, biomecânicos, luminosos, térmicos, e outros, e produzir materiais à temperatura ambiente. As pos
Publicado em: 2010
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3. Controle da fluorescência excitada por dois fótons no polímero conjugado MEH-PPV através da formatação pulsos ultracurtos / Control of the two-photon excited fluorescence in the conjugated polymer MEH-PPV by pulse shaping
Neste trabalho foi investigado o controle do processo de fluorescência excitada por absorção de dois fótons no polímero conjugado MEH-PPV, utilizando um sistema de formatação espectral da fase dos pulsos ultracurtos. Para tal estudo, foi utilizado um oscilador laser de Ti:Safira (15 fs, ~ 800 nm e largura de banda de 60 nm). Através de dois distintos
Publicado em: 2007
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4. Controle coerente em Ãtomos de Rb por manipulaÃÃo da fase de pulsos ultracurtos
Coherent quantum control aims to drive a quantum system towards a desired final state. Nowadays its study is widely employed to manipulate transitions in atomic systems, to break molecular bonds and to optimize the output of chemical reactions, for instance. In this thesis experimental and theoretical results of partial temporal coherent control in two diffe
Publicado em: 2005
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5. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images with the resolution and detail of standard histology without the use of exogenous stains. Imaging of intrinsic indicators within tissue, such as nicotinamide aden
National Academy of Sciences.
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6. Three-photon induced fluorescence of the calcium probe Indo-1.
We report the calcium-dependent emission spectral properties of the calcium probe Indo-1 for three-photon excitation. We found that Indo-1 could be readily excited with the femtosecond pulses from a mode-locked Ti:sapphire laser at 885 nm. This wavelength is too long for two-photon excitation, which is expected to occur for wavelengths no longer than twice t
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7. New separation-free assay technique for SNPs using two-photon excitation fluorometry
A new separation-free method for detection of single nucleotide polymorphisms (SNPs) is described. The method is based on the single base extension principle, fluorescently labeled dideoxy nucleotides and two-photon fluorescence excitation technology, known as ArcDia™ TPX technology. In this assay technique, template-directed single base extension is carri
Oxford University Press.
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8. Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
Multiphoton microscopy relies on nonlinear light–matter interactions to provide contrast and optical sectioning capability for high-resolution imaging. Most multiphoton microscopy studies in biological systems have relied on two-photon excited fluorescence (TPEF) to produce images. With increasing applications of multiphoton microscopy to thick-tissue “i
National Academy of Sciences.
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9. Two types of calcium response limited to single spines in cerebellar Purkinje cells.
Of fundamental importance in understanding neuronal function is the unambiguous determination of the smallest unit of neuronal integration. It was recently suggested that a whole dendritic branchlet, including tens of spines, acts as the fundamental unit in terms of dendritic calcium dynamics in Purkinje cells. By contrast, we demonstrate that the smallest s
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10. Physiological monitoring of optically trapped cells: assessing the effects of confinement by 1064-nm laser tweezers using microfluorometry.
We report the results of microfluorometric measurements of physiological changes in optically trapped immotile Chinese hamster ovary cells (CHOs) and motile human sperm cells under continuous-wave (CW) and pulsed-mode trapping conditions at 1064 nm. The fluorescence spectra derived from the exogenous fluorescent probes laurdan, acridine orange, propidium iod
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11. Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution
Water-soluble quantum dots (qdots) are now being used in life sciences research to take advantage of their bright, easily excited fluorescence and high photostability. Although the frequent erratic blinking and substantial dark (never radiant) fractions that occur in all available qdots may interfere with many applications, these properties of individual par
National Academy of Sciences.
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12. Methicillin-Resistant Staphylococcus aureus Screening by Online Immunometric Monitoring of Bacterial Growth under Selective Pressure▿
Rapid, high-throughput screening tools are needed to contain the spread of hospital-acquired methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) strains. Most techniques used in current clinical practice still require time-consuming culture for primary isolation of the microbe. We present a new phenotypic assay for MRSA screening. The technique e
American Society for Microbiology (ASM).