Type Four Secretion System
Mostrando 1-12 de 32 artigos, teses e dissertações.
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1. Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a
Braz. J. Microbiol.. Publicado em: 2013-09
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2. Genotypic and phenotypic characterization of nonadherent mutants of atypical enteropathogenic Escherichia coli of serotype 0125ac:H6. / Caracterização genotípica e fenotípica de mutantes não aderentes de Escherichia coli enteropatogênica atípica do sorotipo O125ac:H6.
The serotype O125ac:H6 comprises atypical Enteropathogenic Escherichia coli strains that express the aggregative adherence (AA) pattern to HEp-2 cells. We obtained four nonadherent mutants using TnphoA insertion in the Ec292/84 strain. The aim of this study was the genetic and phenotypic characterization of these mutants. The genetic analysis of the mutants
Publicado em: 2009
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3. Strutural characterization of hypothetical proteins complex - XACb0032/XACb0033 from Xanthomonas axonopodis pv. citri / Caracterização estrutural do complexo de proteinas hipoteticas - XACb0032/XACb0033 da bacteria Xanthomonas axonopodis pv. citri
Xanthomonas axonopodis pv. citri (Xac) is the causative agent of citrus canker, a disease of significant economic importance worldwide. The molecular bases of the virulence mechanism are still unknown, but is believed that transfer of bacterial virulence proteins directly into the host cell cytoplasm is mediated by protein secretion systems, mainly type III
Publicado em: 2007
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4. Vibrio cholerae Strains with Mutations in an Atypical Type I Secretion System Accumulate RTX Toxin Intracellularly
This study shows that the Vibrio cholerae RTX toxin is secreted by a four-component type I secretion system (TISS) encoded by rtxB, rtxD, rtxE, and tolC. ATP-binding site mutations in both RtxB and RtxE blocked secretion, demonstrating that this atypical TISS requires two transport ATPases that may function as a heterodimer.
American Society for Microbiology.
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5. Campylobacter fetus Surface Layer Proteins Are Transported by a Type I Secretion System
The virulence of Campylobacter fetus, a bacterial pathogen of ungulates and humans, is mediated in part by the presence of a paracrystalline surface layer (S-layer) that confers serum resistance. The subunits of the S-layer are S-layer proteins (SLPs) that are secreted in the absence of an N-terminal signal sequence and attach to either type A or B C. fetus
American Society for Microbiology.
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6. Single-Nucleotide-Polymorphism Mapping of the Pseudomonas aeruginosa Type III Secretion Toxins for Development of a Diagnostic Multiplex PCR System
We mapped the coding single nucleotide polymorphisms in four toxin genes—exoS, exoT, exoU, and exoY—of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were
American Society for Microbiology.
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7. Neutral metalloproteinases produced by human mononuclear phagocytes. Enzyme profile, regulation, and expression during cellular development.
Mononuclear phagocytes are developmentally and functionally complex cells that play critical roles in extracellular matrix remodeling. We hypothesized that differentiated mononuclear phagocytes, typified by alveolar macrophages, use a spectrum of metalloproteinases to degrade various matrix macromolecules. To test this hypothesis, we have evaluated synthesis
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8. Effector ExoU from the Type III Secretion System Is an Important Modulator of Gene Expression in Lung Epithelial Cells in Response to Pseudomonas aeruginosa Infection
Pseudomonas aeruginosa is an important pathogen in immunocompromised patients and secretes a diverse set of virulence factors that aid colonization and influence host cell defenses. An important early step in the establishment of infection is the production of type III-secreted effectors translocated into host cells by the bacteria. We used cDNA microarrays
American Society for Microbiology.
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9. Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system
Non-O1, non-O139 Vibrio cholerae can cause gastroenteritis and extraintestinal infections, but, unlike O1 and O139 strains of V. cholerae, little is known about the virulence gene content of non-O1, non-O139 strains and their phylogenetic relationship to other pathogenic V. cholerae. Comparative genomic microarray analysis of four pathogenic non-O1, non-O139
National Academy of Sciences.
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10. Salmonella enterica Serovar Typhimurium Exploits Toll-Like Receptor Signaling during the Host-Pathogen Interaction▿ †
Salmonella survives and replicates in host cells by using a type III secretion system to evade host immune defenses. The innate immune system plays an important role as a first line of defense against pathogens and is mediated in part by Toll-like receptors (TLRs); however, the infection dynamics of Salmonella enterica serovar Typhimurium within macrophages
American Society for Microbiology (ASM).
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11. YopD of Yersinia pestis Plays a Role in Negative Regulation of the Low-Calcium Response in Addition to Its Role in Translocation of Yops
Yersinia pestis produces a set of virulence proteins (Yops and LcrV) that are expressed at high levels and secreted by a type III secretion system (Ysc) upon bacterium-host cell contact, and four of the Yops are vectorially translocated into eukaryotic cells. YopD, YopB, and YopK are required for the translocation process. In vitro, induction and secretion o
American Society for Microbiology.
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12. Use of the Galleria mellonella Caterpillar as a Model Host To Study the Role of the Type III Secretion System in Pseudomonas aeruginosa Pathogenesis
Nonvertebrate model hosts represent valuable tools for the study of host-pathogen interactions because they facilitate the identification of bacterial virulence factors and allow the discovery of novel components involved in host innate immune responses. In this report, we determined that the greater wax moth caterpillar Galleria mellonella is a convenient n
American Society for Microbiology.