Udp D Xylose
Mostrando 1-12 de 13 artigos, teses e dissertações.
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1. UDP-N-acetilglicosamina enolpiruvil transferase: determinação dos estados de protonação de resíduos de aminoácidos do sítio ativo pelo método PM6
UDP-N-acetylglucosamine-enolpyruvyl transferase (MurA) catalyzes the reaction between phosphoenol pyruvate and UDP-N-acetylglucosamine. We present a theoretical approach using the semiempirical PM6 method for defining protonation state of three active site residues, K22, H125, and K160. Prior comparison with neutron diffraction data showed that PM6 accuratel
Quím. Nova. Publicado em: 2012
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2. Alteration in the composition of cell wall polysaccharides in Nicotina tabacum by modulating the expression of the uxs gene, coding for UDP-D-glucuronic acid decarboxylase enzyme (EC 4.1.1.35) / Alteração da composição dos polissacarídeos da parede celular de Nicotiana tabacum, pela modulação da expressão do gene uxs que codifica a enzima UDP-D-glucuronato descarboxilase (EC 4.1.1.35)
The plant cell wall is not only an essential structure for plants, but also an extremely important raw material in human economy. The plant cell wall has diverse utilities, for example, papermaking, textile fiber, civil construction. Polysaccharides, such as cellulose, hemicelluloses and pectins, are the major components of the primary plant cell wall (appro
Publicado em: 2007
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3. Nucleoside Diphosphate-Sugar 4-Epimerases: II. Uridine Diphosphate Arabinose 4-Epimerase of Wheat Germ 1
Uridine diphosphate (UDP)-arabinose 4-epimerase (EC 5.1.3.5) has been purified at least 20-fold from wheat germ by MnCl2 treatment, (NH4)2SO4 fractionation, dialysis, and Sephadex and diethylaminoethyl cellulose column chromatography. The enzyme has no action on UDP-d-glucose, UDP-d-glucuronic acid, or TDP-d-glucose. The pH optimum is 8.0. Km values are 1.5
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4. Characterization and Expression Patterns of UDP-d-Glucuronate Decarboxylase Genes in Barley1[w]
UDP-d-glucuronate decarboxylase (EC 4.1.1.35) catalyzes the synthesis of UDP-d-xylose from UDP-d-glucuronate in an essentially irreversible reaction that is believed to commit glycosyl residues to heteroxylan and xyloglucan biosynthesis. Four members of the barley (Hordeum vulgare) UDP-d-glucuronate decarboxylase gene family, designated HvUXS1 to HvUXS4, hav
American Society of Plant Biologists.
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5. The mur4 Mutant of Arabidopsis Is Partially Defective in the de Novo Synthesis of Uridine Diphospho l-Arabinose1
To obtain information on the synthesis and function of arabinosylated glycans, the mur4 mutant of Arabidopsis was characterized. This mutation leads to a 50% reduction in the monosaccharide l-arabinose in most organs and affects arabinose-containing pectic cell wall polysaccharides and arabinogalactan proteins. Feeding l-arabinose to mur4 plants restores the
American Society of Plant Physiologists.
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6. Xylosylation of Phenolic Hydroxyl Groups of the Monomeric Lignin Model Compounds 4-Methylguaiacol and Vanillyl Alcohol by Coriolus versicolor
When 4-methylguaiacol (MeG), a phenolic lignin model compound, was added to a culture that was inoculated with Coriolus versicolor, it was bioconverted into 2-methoxy-4-methylphenyl β-d-xyloside (MeG-Xyl). The phenolic hydroxyl group of vanillyl alcohol was much more extensively xylosylated than the alcoholic hydroxyl group. When a mixture of MeG and commer
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7. Biosynthesis of UDP-Xylose. Cloning and Characterization of a Novel Arabidopsis Gene Family, UXS, Encoding Soluble and Putative Membrane-Bound UDP-Glucuronic Acid Decarboxylase Isoforms
UDP-xylose (Xyl) is an important sugar donor for the synthesis of glycoproteins, polysaccharides, various metabolites, and oligosaccharides in animals, plants, fungi, and bacteria. UDP-Xyl also feedback inhibits upstream enzymes (UDP-glucose [Glc] dehydrogenase, UDP-Glc pyrophosphorylase, and UDP-GlcA decarboxylase) and is involved in its own synthesis and t
American Society of Plant Biologists.
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8. The Biosynthesis of l-Arabinose in Plants: Molecular Cloning and Characterization of a Golgi-Localized UDP-d-Xylose 4-Epimerase Encoded by the MUR4 Gene of Arabidopsis
The mur4 mutant of Arabidopsis shows a 50% reduction in the monosaccharide l-Ara in leaf-derived cell wall material because of a partial defect in the 4-epimerization of UDP-d-Xyl to UDP-l-Ara. To determine the genetic lesion underlying the mur4 phenotype, the MUR4 gene was cloned by a map-based procedure and found to encode a type-II membrane protein with s
American Society of Plant Biologists.
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9. Isolation and partial purification of an enzyme catalyzing the formation of O-xylosylzeatin in Phaseolus vulgaris embryos
An enzyme catalyzing the formation of a cytokinin metabolite, an O-pentosyl derivative of zeatin [Lee, Y. H., Mok, M. C., Mok, D. W. S., Griffin, D. A. & Shaw, G. (1985) Plant Physiol. 77, 635-641], was isolated from Phaseolus vulgaris embryos. Of all the potential pentose donors tested, UDP-xylose was the only substrate recognized by the enzyme. This indica
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10. Cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: UDP-glucose dehydrogenase.
Hemicellulose is a major component of primary plant cell walls. Many of the glycosyl residues found in hemicellulose are derived from the sugar precursor UDP-glucuronic acid, which can be converted into UDP-arabinose, UDP-apiose, UDP-galacturonic acid, and UDP-xylose. The enzyme controlling the biosynthesis of UDP-glucuronic acid, UDP-glucose dehydrogenase (
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11. Arabinoxylan Biosynthesis in Wheat. Characterization of Arabinosyltransferase Activity in Golgi Membranes1
Arabinoxylan arabinosyltransferase (AX-AraT) activity was investigated using microsomes and Golgi vesicles isolated from wheat (Triticum aestivum) seedlings. Incubation of microsomes with UDP-[14C]-β-l-arabinopyranose resulted in incorporation of radioactivity into two different products, although most of the radioactivity was present in xylose (Xyl), indic
American Society of Plant Physiologists.
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12. Control of Chondrogenesis in Limb-Bud Cell Cultures by Bromodeoxyuridine
Initial exposure of cultured limb-bud cells (stage 23-24) to 5-bromo-2′-deoxyuridine (BrdU) irreversibly inhibits differentiation to cartilage under three different culture conditions. The inhibition of chondroitin sulfate synthesis is partially reversed by D-xylose in limb-bud cells after treatment with BrdU. The activities of four enzymes involved in cho