Unigene
Mostrando 1-12 de 58 artigos, teses e dissertações.
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1. Construção de macroarranjos de DNA, a partir do unigene café, para a identificação de genes de interesse agronômico.
2006
ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. Publicado em: 2011
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2. Tandem repeat distribution of gene transcripts in three plant families
Tandem repeats (microsatellites or SSRs) are molecular markers with great potential for plant genetic studies. Modern strategies include the transfer of these markers among widely studied and orphan species. In silico analyses allow for studying distribution patterns of microsatellites and predicting which motifs would be more amenable to interspecies transf
Genetics and Molecular Biology. Publicado em: 06/11/2009
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3. Frequency and distribution of microsatellites from ESTs of citrus
Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus
Genetics and Molecular Biology. Publicado em: 2007
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4. Analise da expressão genica das celulas mononucleares de sangue de cordão umbilical humano : implicação no ciclo celular
A reconstituição da linhagem hematopoiética é um procedimento comum no tratamento de várias doenças hematológicas e não hematológicas. A medula óssea tem sido a principal fonte de obtenção de células tronco nos últimos 50 anos. Recentemente, células tronco obtidas de sangue periférico pós-mobilização e células mononucleares de sangue de c
Publicado em: 2005
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5. PALS db: Putative Alternative Splicing database
PALS db is a collection of Putative Alternative Splicing information from 19 936 human UniGene clusters and 16 615 mouse UniGene clusters. Alternative splicing (AS) sites were predicted by using the longest messenger RNA (mRNA) sequence in each UniGene cluster as the reference sequence. This sequence was aligned with related sequences in UniGene and dbEST to
Oxford University Press.
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6. IMAGE cDNA Clones, UniGene Clustering, and ACeDB: An Integrated Resource for Expressed Sequence Information
In this study we describe a new information resource that provides integrated access to information on IMAGE (integrated molecular analysis of genomes and their expression) cDNA library clones and derived expressed sequence tags (ESTs). We have developed an automated procedure that collates data from various public sources into a single ACeDB database. This
Cold Spring Harbor Laboratory Press.
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7. Transcript identification by analysis of short sequence tags—influence of tag length, restriction site and transcript database
There exist a number of gene expression profiling techniques that utilize restriction enzymes for generation of short expressed sequence tags. We have studied how the choice of restriction enzyme influences various characteristics of tags generated in an experiment. We have also investigated various aspects of in silico transcript identification that these p
Oxford University Press.
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8. In Silico Cloning of Novel Endothelial-Specific Genes
The endothelium plays a pivotal role in many physiological and pathological processes and is known to be an exceptionally active transcriptional site. To advance our understanding of endothelial cell biology and to elucidate potential pharmaceutical targets, we developed a new database screening approach to permit identification of novel endothelial-specific
Cold Spring Harbor Laboratory Press.
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9. d2_cluster: A Validated Method for Clustering EST and Full-Length cDNA Sequences
Several efforts are under way to condense single-read expressed sequence tags (ESTs) and full-length transcript data on a large scale by means of clustering or assembly. One goal of these projects is the construction of gene indices where transcripts are partitioned into index classes (or clusters) such that they are put into the same index class if and only
Cold Spring Harbor Laboratory Press.
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10. GeneHopper: a web-based search engine to link gene-expression platforms through GenBank accession numbers
To compare experiments that are performed on different platforms, array probes and sequence tags need to be linked. A web-based search engine GeneHopper has been developed to link different expression resources based on UniGene clusters and HomoloGene orthologs databases of the National Center for Biotechnology Information (NCBI).
BioMed Central.
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11. Assembly, Annotation, and Integration of UNIGENE Clusters into the Human Genome Draft
The recent release of the first draft of the human genome provides an unprecedented opportunity to integrate human genes and their functions in a complete positional context. However, at least three significant technical hurdles remain: first, to assemble a complete and nonredundant human transcript index; second, to accurately place the individual transcrip
Cold Spring Harbor Laboratory Press.
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12. UniGene cDNA array-based monitoring of transcriptome changes during mouse placental development
The placenta is a highly specialized organ essential for embryonic growth and development. Here, we have applied cDNA subtraction between extraembryonic tissues of early- (day 7.5 of gestation) and late-stage embryos (day 17.5) to generate stage-specific cDNA pools that were used for screening of high-density mouse UniGene cDNA arrays containing 25,000 clone
The National Academy of Sciences.