Venom Extraction
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Preparation and neutralization efficacy of IgY antibodies raised against Deinagkistrodon acutus venom
Abstract Background The five-paced pit viper (Deinagkistrodon acutus), endemic to China and northern Vietnam, is responsible for most snakebites in the Chinese territory. Antivenom produced from horses is the main treatment for snakebites, but it may cause numerous clinical side effects and have other disadvantages involved in their production such as the
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 06/07/2017
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2. Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
Abstract Background The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom produc
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 13/10/2016
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3. Indian scorpions collected in Karnataka: maintenance in captivity, venom extraction and toxicity studies
Abstract Background Maintenance of scorpions under laboratory conditions is ideal for long-term venom collection to explore the therapeutic applications of scorpion venom. Collection of venom by electrical stimulation requires a reliable stimulator and effective restrainer. Thus, the present study was conducted to develop a convenient method to maintain sc
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 31/12/2015
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4. The History of Venomous Spider Identification, Venom Extraction Methods and Antivenom Production: a Long Journey at the Butantan Institute, São Paulo, Brazil
Abstract The article provides a historical report on venomous spider identification, venom obtainment methods and serum production at the Butantan Institute, São Paulo, Brazil. It is based on literature and personnal experience during the last 50 years. This result is the discovery that the real species causing potential severe human accide
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
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5. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
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6. The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects
Background Snakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local t
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 03/03/2015
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7. Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms
Background The search for new inhibitors of snake venom toxins is essential to complement or even replace traditional antivenom therapy, especially in relation to compounds that neutralize the local effects of envenomations. Besides their possible use as alternative to traditional antivenom therapy, some plant species possess bioactive secondary metabolites
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 02/10/2014
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8. Experimental Tityus serrulatus scorpion envenomation: age- and sex-related differences in symptoms and mortality in mice
Among the various methods for evaluating animal venom toxicity, the calculation of the median lethal dose (LD50) is the most widely used. Although different protocols can be used to calculate the LD50, the source of the venom and the method of extraction, as well as the strain, age, and sex of the animal model employed, should be taken into consideration. Th
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2011
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9. Individual variation in the protein profile of the venom of Mesobuthus gibbosus (Brullé, 1832, Scorpiones: Buthidae) from Turkey
Mesobuthus gibbosus (Brullé, 1832) scorpions were collected from the Mugla province in the Aegean region of Turkey and housed in individual boxes. After extraction, the venom composition was analyzed using gel electrophoresis (SDS-PAGE). Interestingly, all scorpion venom samples contained only one protein band (~68 kDa) in common. Two protein bands (30 and
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2010
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10. Preliminary pharmacological studies with the venom obtained from nematocysts of Macrorhynchia philippina (Cnidaria, Hydrozoa) / Estudos farmacológicos preliminares com a peçonha obtida dos nematocistos de Macrorhynchia philippina (Cnidaria, Hydrozoa)
Muitas interações entre organismos são mediadas pela liberação de substâncias biologicamente ativas. Nos Cnidários esse tipo de interação é ainda mais marcante devido a organelas características do grupo, denominadas nematocistos. Usados tanto na captura de alimento quanto para defesa do organismo, os nematocistos contêm toxinas com ações que v
Publicado em: 2010
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11. Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods
Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2007
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12. Determination of serum levels of corticosterone and sexual steroid hormones, stress-induced by physical restraint and venom extraction, on Bothrops jararaca (Ophidia: Viperidae) / Determinação dos níveis séricos de corticosterona e hormônios esteróides sexuais, induzidos pelo estresse da contenção física e da extração de veneno, em Bothrops jararaca (Ophidia: Viperidae).
In the present studies, 30 snakes of the species B. jararaca with three years old, born in captivity at the Laboratório de Herpetologia - Instituto Butantan - were divided into 3 experimental groups, each one containing 10 animals. The mean weight and snout vent length of males and females were 97 grams and 75 centimeters and 281 grams and 99 centimeters, r
Publicado em: 2006